{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2022,7,30]],"date-time":"2022-07-30T02:40:04Z","timestamp":1659148804375},"reference-count":23,"publisher":"Oxford University Press (OUP)","issue":"6","license":[{"start":{"date-parts":[[2013,2,26]],"date-time":"2013-02-26T00:00:00Z","timestamp":1361836800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2013,6,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>Coeliac disease (CD) is characterized by several markers, including anti-transglutaminase auto-antibodies (tTGAb) directed against multiple epitopes of the gliadin protein. We aimed to investigate the correlation among CD duodenal lesions, tTGAb titres and the immunoreactivity against tTG constructs. A total of 345 CD patients (209 females, 136 males, overall median age: 7.3 years) were tested for full-length (fl) tTGAb with a fluid-phase radioimmunoassay. Out of the total, 231 patients were also tested for immunoreactivity against tTG fragments (F1: a.a. 227\u2013687 and F2: a.a. 473\u2013687). Patients were classified according to diffuse (D), patchy (P) or bulb (B) histological lesions. All sera were found fltTGAb positive. Patients with D, P and B lesions had a mean Ab index of 0.84\u00b10.39, 0.57\u00b10.39 and 0.45\u00b10.24, respectively. Mean tTGAb titre varied between D and localized (P+B) patients (0.84\u00b10.39 versus 0.52\u00b10.34, P &amp;lt; 0.0001). Overall, 86.1% of patients were F1 auto-antibody (F1Ab) positive (D: 89%, P: 75%, B: 40%; D versus P+B: P = 0.004) and 49% of patients were F2 auto-antibody (F2Ab) positive (D: 53%, P: 19%, B: 10%; D versus P+B: P = 0.0006). Of the D patients 50.7% showed combined F1Ab-F2Ab (D versus P+B: P = 0.001), whereas 60% of B patients were negative for both F1Ab and F2Ab (B versus D: P &amp;lt; 0.0001). Coeliac-specific tTGAb immunoreactivity correlates with the grading and extension of histological duodenal lesions in CD patients at diagnosis. The immunoreactivity against single and combined tTG fragments is significantly higher in patients with D lesions. This is the first evidence of a distinct coeliac-specific immunoreactivity in patients with different duodenal involvement.<\/jats:p>","DOI":"10.1093\/intimm\/dxs159","type":"journal-article","created":{"date-parts":[[2013,2,28]],"date-time":"2013-02-28T23:02:14Z","timestamp":1362092534000},"page":"389-394","source":"Crossref","is-referenced-by-count":4,"title":["Anti-transglutaminase immunoreactivity and histological lesions of the duodenum in coeliac patients"],"prefix":"10.1093","volume":"25","author":[{"given":"Raffaella","family":"Nenna","sequence":"first","affiliation":[{"name":"Department of Paediatrics 1 , Sapienza University of Rome, Rome, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Claudio","family":"Tiberti","sequence":"additional","affiliation":[{"name":"Clinical Science and Medical Specialties 2 , Sapienza University of Rome, Rome, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Laura","family":"Petrarca","sequence":"additional","affiliation":[{"name":"Department of Paediatrics 1 , Sapienza University of Rome, Rome, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maurizio","family":"Mennini","sequence":"additional","affiliation":[{"name":"Department of Paediatrics 1 , Sapienza University of Rome, Rome, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gerarda","family":"Mastrogiorgio","sequence":"additional","affiliation":[{"name":"Department of Paediatrics 1 , Sapienza University of Rome, Rome, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Federica","family":"Lucantoni","sequence":"additional","affiliation":[{"name":"Department of Paediatrics 1 , Sapienza University of Rome, Rome, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Francesca","family":"Panimolle","sequence":"additional","affiliation":[{"name":"Clinical Science and Medical Specialties 2 , Sapienza University of Rome, Rome, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Stefano","family":"Pontone","sequence":"additional","affiliation":[{"name":"Surgical Sciences 3 , Sapienza University of Rome, Rome, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maria","family":"Bavastrelli","sequence":"additional","affiliation":[{"name":"Department of Paediatrics 1 , Sapienza University of Rome, Rome, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fabio Massimo","family":"Magliocca","sequence":"additional","affiliation":[{"name":"Experimental Medicine and Pathology, Sapienza University of Rome 4 , Rome , Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Margherita","family":"Bonamico","sequence":"additional","affiliation":[{"name":"Department of Paediatrics 1 , Sapienza University of Rome, Rome, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[2013,2,26]]},"reference":[{"key":"2022073002091002800_CIT0001","doi-asserted-by":"crossref","first-page":"797","DOI":"10.1038\/nm0797-797","article-title":"Identification of tissue transglutaminase as the autoantigen of celiac disease","volume":"3","author":"Dieterich","year":"1997","journal-title":"Nat. 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Immunol."},{"key":"2022073002091002800_CIT0004","doi-asserted-by":"crossref","first-page":"5175","DOI":"10.1046\/j.1432-1033.2002.03215.x","article-title":"The analysis of the fine specificity of celiac disease antibodies using tissue transglutaminase fragments","volume":"269","author":"Sblattero","year":"2002","journal-title":"Eur. J. Biochem."},{"key":"2022073002091002800_CIT0005","doi-asserted-by":"crossref","first-page":"53","DOI":"10.1016\/j.jaut.2003.09.002","article-title":"Epitopes recognised by tissue transglutaminase antibodies in coeliac disease","volume":"22","author":"Nakachi","year":"2004","journal-title":"J. Autoimmun."},{"key":"2022073002091002800_CIT0006","doi-asserted-by":"crossref","first-page":"318","DOI":"10.1016\/j.clim.2003.08.010","article-title":"Celiac disease-associated transglutaminase autoantibody target domains at diagnosis are age and sex dependent","volume":"109","author":"Tiberti","year":"2003","journal-title":"Clin. 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Gastroenterol."},{"key":"2022073002091002800_CIT0012","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1097\/00005176-200501000-00001","article-title":"Guideline for the diagnosis and treatment of celiac disease in children: recommendations of the North American Society for Pediatric Gastroenterology, Hepatology and Nutrition","volume":"40","author":"Hill","year":"2005","journal-title":"J. Pediatr. Gastroenterol. 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