{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,12,1]],"date-time":"2023-12-01T05:43:37Z","timestamp":1701409417053},"reference-count":39,"publisher":"Oxford University Press (OUP)","issue":"10","license":[{"start":{"date-parts":[[2017,5,22]],"date-time":"2017-05-22T00:00:00Z","timestamp":1495411200000},"content-version":"vor","delay-in-days":1096,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2014,10,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>NKT cells are characterized by their expression of an NKT-cell-specific invariant antigen-receptor \u03b1 chain encoded by V\u03b114J\u03b118 gene segments. These NKT cells bridge the innate and acquired immune systems to mediate effective and augmented responses; however, the limited number of NKT cells in vivo hampers their analysis. Here, two lines of induced pluripotent stem cell-derived mice (NKT-iPSC-derived mice) were generated by reprogramming of mature NKT cells, where one harbors both rearranged V\u03b114J\u03b118 and V\u03b27 genes and the other carries rearranged V\u03b114J\u03b118 on both alleles but germline V\u03b2 loci. The analysis of NKT-iPSC-derived mice showed a significant increase in NKT cell numbers with relatively normal frequencies of functional subsets, but significantly enhanced in some cases, and acquired functional NKT cell maturation in peripheral lymphoid organs. NKT-iPSC-derived mice also showed normal development of other immune cells except for the absence of \u03b3\u03b4T cells and disturbed development of conventional CD4 \u03b1\u03b2T cells. These results suggest that the NKT-iPSC-derived mice are a better model for NKT cell development and function study rather than transgenic mouse models reported previously and also that the presence of a pre-rearranged V\u03b114J\u03b118 in the natural chromosomal context favors the developmental fate of NKT cells.<\/jats:p>","DOI":"10.1093\/intimm\/dxu057","type":"journal-article","created":{"date-parts":[[2014,5,23]],"date-time":"2014-05-23T03:26:43Z","timestamp":1400815603000},"page":"551-561","source":"Crossref","is-referenced-by-count":6,"title":["Generation of induced pluripotent stem cell-derived mice by reprogramming of a mature NKT cell"],"prefix":"10.1093","volume":"26","author":[{"given":"Yue","family":"Ren","sequence":"first","affiliation":[{"name":"Laboratory for Immune Regulation, RCAI, RIKEN Center for Integrative Medical Sciences (IMS-RCAI) 1 , 230-0045 Kanagawa , Japan"},{"name":"The Key Laboratory of Pathobiology, Ministry of Education, Jilin University, Changchun 2 , 130021 Jilin , People\u2019s Republic of China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nyambayar","family":"Dashtsoodol","sequence":"additional","affiliation":[{"name":"Laboratory for Immune Regulation, RCAI, RIKEN Center for Integrative Medical Sciences (IMS-RCAI) 1 , 230-0045 Kanagawa , Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hiroshi","family":"Watarai","sequence":"additional","affiliation":[{"name":"Laboratory for Immune Regulation, RCAI, RIKEN Center for Integrative Medical Sciences (IMS-RCAI) 1 , 230-0045 Kanagawa , Japan"},{"name":"Precursory Research for Embryonic Science and Technology (PRESTO), Japan Science and Technology Agency (JST) 3 , 102-0076 Tokyo , Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Haruhiko","family":"Koseki","sequence":"additional","affiliation":[{"name":"Laboratory for Developmental Genetics, RCAI, RIKEN Center for Integrative Medical Sciences (IMS-RCAI) 4 , 230-0045 Kanagawa , Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chengshi","family":"Quan","sequence":"additional","affiliation":[{"name":"The Key Laboratory of Pathobiology, Ministry of Education, Jilin University, Changchun 2 , 130021 Jilin , People\u2019s Republic of China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Masaru","family":"Taniguchi","sequence":"additional","affiliation":[{"name":"Laboratory for Immune Regulation, RCAI, RIKEN Center for Integrative Medical Sciences (IMS-RCAI) 1 , 230-0045 Kanagawa , Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[2014,5,22]]},"reference":[{"key":"2023120105143561200_CIT0001","doi-asserted-by":"crossref","first-page":"483","DOI":"10.1146\/annurev.immunol.21.120601.141057","article-title":"The regulatory role of Valpha14 NKT cells in innate and acquired immune response","volume":"21","author":"Taniguchi","year":"2003","journal-title":"Annu. 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