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To establish a new murine model of myositis inducible with a single CD8 T-cell epitope peptide that derives from the C protein, three internet-based prediction systems were employed to identify 24 candidate peptides of the immunogenic fragment of the C protein and bind theoretically to major histocompatibility complex class I molecules of C57BL\/6 (B6) mice. RMA-S cell assay revealed that a HILIYSDV peptide, amino acid position 399\u2013406 of the C protein, had the highest affinity to the H2-Kb molecules. Transfer of mature bone marrow-derived dendritic cells pulsed with HILIYSDV induced myositis in naive B6 mice. This myositis was suppressed by anti-CD8-depleting antibodies but not by anti-CD4-depleting antibodies. Because this myositis model is mediated by CD8 T cells independently of CD4 T cells, it should be a useful tool to investigate pathology of polymyositis and develop therapies targeting CD8 T cells.<\/jats:p>","DOI":"10.1093\/intimm\/dxv001","type":"journal-article","created":{"date-parts":[[2015,1,11]],"date-time":"2015-01-11T01:44:25Z","timestamp":1420940665000},"page":"327-332","source":"Crossref","is-referenced-by-count":12,"title":["Experimental myositis inducible with transfer of dendritic cells presenting a skeletal muscle C protein-derived CD8 epitope peptide"],"prefix":"10.1093","volume":"27","author":[{"given":"Naoko","family":"Okiyama","sequence":"first","affiliation":[{"name":"Department of Rheumatology, Tokyo Medical and Dental University (TMDU) 1 , Bunkyo-ku, Tokyo 113-8519 , Japan"},{"name":"Department of Dermatology, Graduate School of Medical and Dental Sciences, Tokyo Medical and Dental University (TMDU) 2 , Bunkyo-ku, Tokyo 113-8519 , Japan"},{"name":"Japan Society for the Promotion of Science 3 , Tokyo 102-0083 , Japan"},{"name":"Research Center for Allergy and Immunology, RIKEN Yokohama Institute 4 , Yokohama, Kanagawa 230-0045 , Japan"},{"name":"Department of Dermatology, Faculty of Medicine, University of Tsukuba 5 , Tsukuba, Ibarakii 305-8575 , Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hisanori","family":"Hasegawa","sequence":"additional","affiliation":[{"name":"Department of Rheumatology, Tokyo Medical and Dental University (TMDU) 1 , Bunkyo-ku, Tokyo 113-8519 , Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Takatoku","family":"Oida","sequence":"additional","affiliation":[{"name":"Japan Society for the Promotion of Science 3 , Tokyo 102-0083 , Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shinya","family":"Hirata","sequence":"additional","affiliation":[{"name":"Department of Rheumatology, Tokyo Medical and Dental University (TMDU) 1 , Bunkyo-ku, Tokyo 113-8519 , Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hiroo","family":"Yokozeki","sequence":"additional","affiliation":[{"name":"Department of Dermatology, Graduate School of Medical and Dental Sciences, Tokyo Medical and Dental University (TMDU) 2 , Bunkyo-ku, Tokyo 113-8519 , Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Manabu","family":"Fujimoto","sequence":"additional","affiliation":[{"name":"Department of Dermatology, Faculty of Medicine, University of Tsukuba 5 , Tsukuba, Ibarakii 305-8575 , Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nobuyuki","family":"Miyasaka","sequence":"additional","affiliation":[{"name":"Department of Rheumatology, Tokyo Medical and Dental University (TMDU) 1 , Bunkyo-ku, Tokyo 113-8519 , Japan"},{"name":"Global Center of Excellence Program, International Research Center for Molecular Science in Tooth and Bone Diseases, Graduate School of Medical and Dental Sciences, Tokyo Medical and Dental University (TMDU) 6 , Bunkyo-ku, Tokyo 113-8519 , Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hitoshi","family":"Kohsaka","sequence":"additional","affiliation":[{"name":"Department of Rheumatology, Tokyo Medical and Dental University (TMDU) 1 , Bunkyo-ku, Tokyo 113-8519 , Japan"},{"name":"Research Center for Allergy and Immunology, RIKEN Yokohama Institute 4 , Yokohama, Kanagawa 230-0045 , Japan"},{"name":"Global Center of Excellence Program, International Research Center for Molecular Science in Tooth and Bone Diseases, Graduate School of Medical and Dental Sciences, Tokyo Medical and Dental University (TMDU) 6 , Bunkyo-ku, Tokyo 113-8519 , Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[2015,1,10]]},"reference":[{"key":"2022072019584474900_CIT0001","doi-asserted-by":"crossref","first-page":"971","DOI":"10.1016\/S0140-6736(03)14368-1","article-title":"Polymyositis and dermatomyositis","volume":"362","author":"Dalakas","year":"2003","journal-title":"Lancet"},{"key":"2022072019584474900_CIT0002","doi-asserted-by":"crossref","first-page":"193","DOI":"10.1002\/ana.410160206","article-title":"Monoclonal antibody analysis of mononuclear cells in myopathies. 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