{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T02:37:45Z","timestamp":1790649465953,"version":"4.1.0"},"reference-count":20,"publisher":"Oxford University Press (OUP)","issue":"8","license":[{"start":{"date-parts":[[2017,8,1]],"date-time":"2017-08-01T00:00:00Z","timestamp":1501545600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2017,8,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>While NLRC4-dependent sensing of intracellular Gram-negative pathogens such as Salmonella enterica serovar typhimurium is a beneficial host response, NLRC4-dependent sensing of the Pseudomonas aeruginosa type 3 secretion system (T3SS) has been shown to be involved in pathogenicity. In mice, different pathogen-associated microbial patterns are sensed by the combination of the NLRC4-inflammasome with different neuronal apoptosis inhibitory proteins (NAIPs). NAIP2 is involved in sensing PscI, an inner-rod protein of the P. aeruginosa T3SS. Surprisingly, only a single human NAIP (hNAIP) has been found. Moreover, there is no description of hNAIP-NLRC4 inflammasome recognition of T3SS inner-rod proteins in humans. Here, we show that the P. aeruginosa T3SS inner-rod protein PscI and needle protein PscF are both sensed by the hNAIP-NLRC4 inflammasome in human macrophages and PBMCs from healthy donors, allowing caspase-1 and IL-1\u03b2 maturation and resulting in a robust inflammatory response. TLR4 and TLR2 are involved in redundantly sensing these two T3SS components.<\/jats:p>","DOI":"10.1093\/intimm\/dxx047","type":"journal-article","created":{"date-parts":[[2017,8,26]],"date-time":"2017-08-26T11:08:22Z","timestamp":1503745702000},"page":"377-384","source":"Crossref","is-referenced-by-count":52,"title":["The human NAIP-NLRC4-inflammasome senses the <i>Pseudomonas aeruginosa<\/i> T3SS inner-rod protein"],"prefix":"10.1093","volume":"29","author":[{"given":"Teddy","family":"Grandjean","sequence":"first","affiliation":[{"name":"EA 7366, Host-Pathogens Translational Research Group, Faculty of Medicine of Lille, University of Lille Nord de France 1 , F-59000 Lille , France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anne","family":"Boucher","sequence":"additional","affiliation":[{"name":"EA 7366, Host-Pathogens Translational Research Group, Faculty of Medicine of Lille, University of Lille Nord de France 1 , F-59000 Lille , France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marion","family":"Thepaut","sequence":"additional","affiliation":[{"name":"EA 7366, Host-Pathogens Translational Research Group, Faculty of Medicine of Lille, University of Lille Nord de France 1 , F-59000 Lille , France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Laura","family":"Monlezun","sequence":"additional","affiliation":[{"name":"Universit\u00e9 Grenoble Alpes 2 , F-38041 Grenoble , France"},{"name":"CNRS, Bacterial Pathogenesis and Cellular Responses 3 , ERL 5261, F-38054 Grenoble , France"},{"name":"INSERM, UMR-S 1036, Biology of Cancer and Infection 4 , F-38054 Grenoble , France"},{"name":"CEA, DSV\/iRTSV 5 , F-38054 Grenoble , France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Benoit","family":"Guery","sequence":"additional","affiliation":[{"name":"EA 7366, Host-Pathogens Translational Research Group, Faculty of Medicine of Lille, University of Lille Nord de France 1 , F-59000 Lille , France"},{"name":"Infectious Diseases Service, Centre Hospitalier Universitaire Vaudois and University of Lausanne 6 , CH-1011 Lausanne , Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eric","family":"Faudry","sequence":"additional","affiliation":[{"name":"Universit\u00e9 Grenoble Alpes 2 , F-38041 Grenoble , France"},{"name":"CNRS, Bacterial Pathogenesis and Cellular Responses 3 , ERL 5261, F-38054 Grenoble , France"},{"name":"INSERM, UMR-S 1036, Biology of Cancer and Infection 4 , F-38054 Grenoble , France"},{"name":"CEA, DSV\/iRTSV 5 , F-38054 Grenoble , France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eric","family":"Kipnis","sequence":"additional","affiliation":[{"name":"EA 7366, Host-Pathogens Translational Research Group, Faculty of Medicine of Lille, University of Lille Nord de France 1 , F-59000 Lille , France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rodrigue","family":"Dessein","sequence":"additional","affiliation":[{"name":"EA 7366, Host-Pathogens Translational Research Group, Faculty of Medicine of Lille, University of Lille Nord de France 1 , F-59000 Lille , France"},{"name":"Microbiological Institute, Center of Biology and Pathology, Teaching University Hospital of Lille, Univ Lille Nord de France 7 , F-59000 Lille , France"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2017,8,28]]},"reference":[{"key":"2022093019003429200_CIT0001","doi-asserted-by":"crossref","first-page":"344","DOI":"10.1097\/01.CCM.0000194725.48928.3A","article-title":"Sepsis in European intensive care units: results of the SOAP study","volume":"34","author":"Vincent","year":"2006","journal-title":"Crit. 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