{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,28]],"date-time":"2026-10-28T09:03:52Z","timestamp":1793178232518,"version":"4.5.0"},"reference-count":40,"publisher":"Oxford University Press (OUP)","issue":"10","license":[{"start":{"date-parts":[[2017,10,1]],"date-time":"2017-10-01T00:00:00Z","timestamp":1506816000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"funder":[{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","award":["20113003"],"award-info":[{"award-number":["20113003"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001700","name":"Ministry of Education, Culture, Sports, Science and Technology","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001700","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2017,12,18]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Nasopharynx-associated lymphoid tissue (NALT) is one of the major constituents of the mucosa-associated lymphoid tissue (MALT), and has the ability to induce antigen-specific immune responses. However, the molecular mechanisms responsible for antigen uptake from the nasal cavity into the NALT remain largely unknown. Immunohistochemical analysis showed that CCL9 and CCL20 were co-localized with glycoprotein 2 (GP2) in the epithelium covering NALT, suggesting the existence of M cells in NALT. In analogy with the reduced number of Peyer\u2019s patch M cells in CCR6-deficient mice, the number of NALT M cells was drastically decreased in CCR6-deficient mice compared with the wild-type mice. Translocation of nasally administered Salmonella enterica serovar Typhimurium into NALT via NALT M cells was impaired in CCR6-deficient mice, whereas S. Typhimurium demonstrated consistent co-localization with NALT M cells in wild-type mice. When wild-type mice were nasally administered with an attenuated vaccine strain of S. Typhimurium, the mice were protected from a subsequent challenge with wild-type S. Typhimurium. Antigen-specific fecal and nasal IgA was detected after nasal immunization with the attenuated vaccine strain of S. Typhimurium only in wild-type mice but not in CCR6-deficient mice. Taken together, these observations demonstrate that NALT M cells are important as a first line of defense against infection by enabling activation of the common mucosal immune system (CMIS).<\/jats:p>","DOI":"10.1093\/intimm\/dxx064","type":"journal-article","created":{"date-parts":[[2017,11,26]],"date-time":"2017-11-26T12:07:27Z","timestamp":1511698047000},"page":"471-478","source":"Crossref","is-referenced-by-count":79,"title":["NALT M cells are important for immune induction for the common mucosal immune system"],"prefix":"10.1093","volume":"29","author":[{"given":"Yasuhiro","family":"Date","sequence":"first","affiliation":[{"name":"RIKEN Center for Integrative Medical Sciences (IMS), Kanagawa, Japan"},{"name":"Graduate School of Medical Life Science, Yokohama City University, Kanagawa, Japan"},{"name":"RIKEN Center for Sustainable Resource Science, Kanagawa, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Masashi","family":"Ebisawa","sequence":"additional","affiliation":[{"name":"RIKEN Center for Integrative Medical Sciences (IMS), Kanagawa, Japan"},{"name":"Graduate School of Medical Life Science, Yokohama City University, Kanagawa, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shinji","family":"Fukuda","sequence":"additional","affiliation":[{"name":"RIKEN Center for Integrative Medical Sciences (IMS), Kanagawa, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hideaki","family":"Shima","sequence":"additional","affiliation":[{"name":"RIKEN Center for Integrative Medical Sciences (IMS), Kanagawa, Japan"},{"name":"Graduate School of Medical Life Science, Yokohama City University, Kanagawa, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuuki","family":"Obata","sequence":"additional","affiliation":[{"name":"RIKEN Center for Integrative Medical Sciences (IMS), Kanagawa, Japan"},{"name":"Graduate School of Medicine, Chiba University, Chiba, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daisuke","family":"Takahashi","sequence":"additional","affiliation":[{"name":"RIKEN Center for Integrative Medical Sciences (IMS), Kanagawa, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tamotsu","family":"Kato","sequence":"additional","affiliation":[{"name":"RIKEN Center for Integrative Medical Sciences (IMS), Kanagawa, Japan"},{"name":"Graduate School of Medical Life Science, Yokohama City University, Kanagawa, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Misaho","family":"Hanazato","sequence":"additional","affiliation":[{"name":"RIKEN Center for Integrative Medical Sciences (IMS), Kanagawa, Japan"},{"name":"Graduate School of Medical Life Science, Yokohama City University, Kanagawa, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gaku","family":"Nakato","sequence":"additional","affiliation":[{"name":"RIKEN Center for Integrative Medical Sciences (IMS), Kanagawa, Japan"},{"name":"Graduate School of Medical Life Science, Yokohama City University, Kanagawa, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ifor R","family":"Williams","sequence":"additional","affiliation":[{"name":"Department of Pathology, Emory University School of Medicine, Atlanta, GA, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Koji","family":"Hase","sequence":"additional","affiliation":[{"name":"RIKEN Center for Integrative Medical Sciences (IMS), Kanagawa, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8776-9661","authenticated-orcid":false,"given":"Hiroshi","family":"Ohno","sequence":"additional","affiliation":[{"name":"RIKEN Center for Integrative Medical Sciences (IMS), Kanagawa, Japan"},{"name":"Graduate School of Medical Life Science, Yokohama City University, Kanagawa, Japan"},{"name":"Graduate School of Medicine, Chiba University, Chiba, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2017,11,27]]},"reference":[{"key":"2022082522173866400_CIT0001","doi-asserted-by":"crossref","first-page":"666","DOI":"10.1038\/mi.2013.30","article-title":"Microfold (M) cells: important immunosurveillance posts in the intestinal epithelium","volume":"6","author":"Mabbott","year":"2013","journal-title":"Mucosal Immunol"},{"key":"2022082522173866400_CIT0002","doi-asserted-by":"crossref","first-page":"151","DOI":"10.1093\/jb\/mvv121","article-title":"Intestinal M cells","volume":"159","author":"Ohno","year":"2016","journal-title":"J. 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