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In this study, we defined the PP lymphoid structures and immune cells required for the induction of mucosal SIgA antibody responses. Adoptive transfer of mononuclear cells (MNCs) from PPs into PP-deficient (PP-null) mice failed to elicit tetanus toxoid (TT)-specific mucosal immunity. However, when the same PP MNCs were transferred into lethally irradiated PP-normal recipient mice, PP MNCs preferentially emigrated to recipient PPs, leading to PP lymphoid structures and TT-specific SIgA antibody responses. Significantly reduced numbers of TT-specific IgA antibody-forming cells were detected in the mesenteric lymph nodes (MLNs) and intestinal lamina propria of mice when surface expression of the sphingosine 1-phosphate receptor on lymphocytes was inhibited by its agonist FTY720. However, FTY720 treatment did not alter dendritic cell migration or Salmonella dissemination into these tissues. When rSalmonella-Tox C-stimulated CD4+ T cells isolated from PPs, MLNs and the spleen were co-cultured with B cells from these tissues, significantly increased levels of TT-specific IgA antibody responses were exclusively induced in cultures containing PP B cells. Furthermore, surface IgA+ PP B cells produced TT-specific IgA antibody responses in vitro. These findings suggest that PP lymphoid structures and surface IgA+ PP B cells are essential elements for the induction of antigen-specific intestinal SIgA antibody responses to oral Salmonella.<\/jats:p>","DOI":"10.1093\/intimm\/dxz029","type":"journal-article","created":{"date-parts":[[2019,3,13]],"date-time":"2019-03-13T12:33:09Z","timestamp":1552480389000},"page":"531-541","source":"Crossref","is-referenced-by-count":10,"title":["Distinct roles for Peyer\u2019s patch B cells for induction of antigen-specific IgA antibody responses in mice administered oral recombinant<i>Salmonella<\/i>"],"prefix":"10.1093","volume":"31","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6323-9535","authenticated-orcid":false,"given":"Tomomi","family":"Hashizume-Takizawa","sequence":"first","affiliation":[{"name":"Department of Microbiology and Immunology, Nihon University School of Dentistry at Matsudo, Matsudo, Chiba, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Naoko","family":"Shibata","sequence":"additional","affiliation":[{"name":"International Research and Development Center for Mucosal Vaccines, The Institute of Medical Science, The University of Tokyo, Minato-ku, Tokyo, Japan"},{"name":"Faculty of Science and Engineering, Waseda University, 513 Wasedatsurumakicho, Shinjuku-ku, Tokyo, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yosuke","family":"Kurashima","sequence":"additional","affiliation":[{"name":"International Research and Development Center for Mucosal Vaccines, The Institute of Medical Science, The University of Tokyo, Minato-ku, Tokyo, Japan"},{"name":"Division of Mucosal Immunology, The University of Tokyo, Distinguished Professor Unit, The Institute of Medical Science, The University of Tokyo, Minato-ku, Tokyo, Japan"},{"name":"Division of Gastroenterology, Department of Medicine, CU-UCSD Center for Mucosal Immunology, Allergy and Vaccines, University of California, La Jolla, San Diego, CA, USA"},{"name":"Departments of Innovative Medicine and Mucosal Immunology, Graduate School of Medicine, Chiba University, Chuo-ku, Chiba-shi, Chiba, Japan"},{"name":"Laboratory of Vaccine Materials, National Institutes of Biomedical Innovation, Health and Nutrition, Saito, Ibaraki-shi, Osaka, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hiroshi","family":"Kiyono","sequence":"additional","affiliation":[{"name":"International Research and Development Center for Mucosal Vaccines, The Institute of Medical Science, The University of Tokyo, Minato-ku, Tokyo, Japan"},{"name":"Division of Mucosal Immunology, The University of Tokyo, Distinguished Professor Unit, The Institute of Medical Science, The University of Tokyo, Minato-ku, Tokyo, Japan"},{"name":"Division of Gastroenterology, Department of Medicine, CU-UCSD Center for Mucosal Immunology, Allergy and Vaccines, University of California, La Jolla, San Diego, CA, USA"},{"name":"Mucosal Immunology and Allergy Therapeutics, Institute for Global Prominent Research, Graduate School of Medicine, Chiba University, Chuo-ku, Chiba-shi, Chiba, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tomoko","family":"Kurita-Ochiai","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, Nihon University School of Dentistry at Matsudo, Matsudo, Chiba, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kohtaro","family":"Fujihashi","sequence":"additional","affiliation":[{"name":"International Research and Development Center for Mucosal Vaccines, The Institute of Medical Science, The University of Tokyo, Minato-ku, Tokyo, Japan"},{"name":"Department of Pediatric Dentistry, Institute of Oral Health Research, The University of Alabama at Birmingham, SDB, Birmingham, AL, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2019,3,14]]},"reference":[{"key":"2022071822160573000_CIT0001","doi-asserted-by":"crossref","first-page":"239","DOI":"10.1016\/B978-0-7234-3691-1.00040-4","article-title":"Host defenses at mucosal surfaces","volume-title":"Clinical Immunology: Principles and Practice","author":"Fujihashi","year":"2013","edition":"4"},{"key":"2022071822160573000_CIT0002","doi-asserted-by":"crossref","first-page":"505","DOI":"10.1016\/j.it.2008.07.008","article-title":"Immunological commonalities and distinctions between airway and digestive immunity","volume":"29","author":"Kunisawa","year":"2008","journal-title":"Trends Immunol."},{"key":"2022071822160573000_CIT0003","doi-asserted-by":"crossref","first-page":"331","DOI":"10.1038\/nri1057","article-title":"Anatomical basis of tolerance and immunity to intestinal antigens","volume":"3","author":"Mowat","year":"2003","journal-title":"Nat. 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