{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,17]],"date-time":"2026-08-17T23:23:35Z","timestamp":1787009015550,"version":"build-2736575974"},"reference-count":60,"publisher":"Oxford University Press (OUP)","issue":"1","license":[{"start":{"date-parts":[[2024,9,11]],"date-time":"2024-09-11T00:00:00Z","timestamp":1726012800000},"content-version":"vor","delay-in-days":254,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000900","name":"California Institute for Regenerative Medicine","doi-asserted-by":"publisher","award":["EDUC4-12813"],"award-info":[{"award-number":["EDUC4-12813"]}],"id":[{"id":"10.13039\/100000900","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["R01GM135657"],"award-info":[{"award-number":["R01GM135657"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2024,1,9]]},"abstract":"<jats:title>Synopsis<\/jats:title>\n                  <jats:p>Planarians are an excellent model for investigating molecular mechanisms necessary for regenerating a functional nervous system. Numerous studies have led to the generation of extensive genomic resources, especially whole-animal single-cell RNA-seq resources. These have facilitated in silico predictions of neuronal subtypes, many of which have been anatomically mapped by in situ hybridization. However, our knowledge of the function of dozens of neuronal subtypes remains poorly understood. Previous investigations identified that polycystic kidney disease (pkd)-like genes in planarians are strongly expressed in sensory neurons and have roles in mechanosensation. Here, we examine the expression and function of all the pkd genes found in the Schmidtea mediterranea genome and map their expression in the asexual and hermaphroditic strains. Using custom behavioral assays, we test the function of pkd genes in response to mechanical stimulation and in food detection. Our work provides insight into the physiological function of sensory neuron populations and protocols for creating inexpensive automated setups for acquiring and analyzing mechanosensory stimulation in planarians.<\/jats:p>","DOI":"10.1093\/iob\/obae035","type":"journal-article","created":{"date-parts":[[2024,9,7]],"date-time":"2024-09-07T07:35:06Z","timestamp":1725694506000},"source":"Crossref","is-referenced-by-count":12,"title":["The Role of\n                    <i>Polycystic Kidney Disease-Like<\/i>\n                    Homologs in Planarian Nervous System Regeneration and Function"],"prefix":"10.1093","volume":"6","author":[{"given":"K G","family":"Ross","sequence":"first","affiliation":[{"name":"Department of Biology, San Diego State University , 5500 Campanile Dr., San Diego, CA 92182 ,","place":["USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S","family":"Alvarez Zepeda","sequence":"additional","affiliation":[{"name":"Department of Biology, San Diego State University , 5500 Campanile Dr., San Diego, CA 92182 ,","place":["USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M A","family":"Auwal","sequence":"additional","affiliation":[{"name":"Department of Biology, San Diego State University , 5500 Campanile Dr., San Diego, CA 92182 ,","place":["USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A K","family":"Garces","sequence":"additional","affiliation":[{"name":"Department of Biology, San Diego State University , 5500 Campanile Dr., San Diego, CA 92182 ,","place":["USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S","family":"Roman","sequence":"additional","affiliation":[{"name":"Department of Biology, San Diego State University , 5500 Campanile Dr., San Diego, CA 92182 ,","place":["USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6272-0519","authenticated-orcid":false,"given":"R M","family":"Zayas","sequence":"additional","affiliation":[{"name":"Department of Biology, San Diego State University , 5500 Campanile Dr., San Diego, CA 92182 ,","place":["USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2024,9,10]]},"reference":[{"key":"2024100315165965800_bib1","doi-asserted-by":"crossref","first-page":"445","DOI":"10.1007\/978-1-4939-7802-1_17","article-title":"Systemic RNA interference in planarians by feeding of dsRNA containing bacteria","volume":"1774","author":"Adler","year":"2018","journal-title":"Methods Mol Biol"},{"key":"2024100315165965800_bib2","doi-asserted-by":"crossref","first-page":"111","DOI":"10.2307\/1536302","article-title":"Reversibility of the reactions of Planaria dorotocephala to a current of water","volume":"29","author":"Allen","year":"1915","journal-title":"Biol Bull"},{"key":"2024100315165965800_bib3","doi-asserted-by":"crossref","first-page":"777951","DOI":"10.3389\/fcell.2021.777951","article-title":"Regeneration of planarian auricles and reestablishment of chemotactic ability","volume":"9","author":"Almazan","year":"2021","journal-title":"Front Cell Dev Biol"},{"key":"2024100315165965800_bib4","doi-asserted-by":"crossref","first-page":"1686","DOI":"10.1038\/s41593-017-0005-0","article-title":"Activation of planarian TRPA1 by reactive oxygen species reveals a conserved mechanism for animal nociception","volume":"20","author":"Arenas","year":"2017","journal-title":"Nat Neurosci"},{"key":"2024100315165965800_bib5","doi-asserted-by":"crossref","first-page":"6706","DOI":"10.1038\/s41467-021-26986-2","article-title":"Hox genes regulate asexual reproductive behavior and tissue segmentation in adult animals","volume":"12","author":"Arnold","year":"2021","journal-title":"Nat Commun"},{"key":"2024100315165965800_bib6","doi-asserted-by":"crossref","first-page":"1341","DOI":"10.1016\/S0960-9822(01)00423-7","article-title":"The Caenorhabditis elegans autosomal dominant polycystic kidney disease gene homologs lov-1 and pkd-2 act in the same pathway","volume":"11","author":"Barr","year":"2001","journal-title":"Curr Biol"},{"key":"2024100315165965800_bib7","doi-asserted-by":"crossref","first-page":"3691","DOI":"10.1242\/dev.01941","article-title":"Planarian homologs of netrin and netrin receptor are required for proper regeneration of the central nervous system and the maintenance of nervous system architecture","volume":"132","author":"Cebri\u00e0","year":"2005","journal-title":"Development"},{"key":"2024100315165965800_bib8","doi-asserted-by":"crossref","first-page":"6968","DOI":"10.1038\/s41467-022-34630-w","article-title":"Muscle5: high-accuracy alignment ensembles enable unbiased assessments of sequence homology and phylogeny","volume":"13","author":"Edgar","year":"2022","journal-title":"Nat Commun"},{"key":"2024100315165965800_bib9","first-page":"5","article-title":"Identification and expression analysis of the complete family of Zebrafish pkd","volume":"5","author":"England","year":"2017","journal-title":"genes. Front Cell Dev Biol"},{"key":"2024100315165965800_bib10","doi-asserted-by":"crossref","first-page":"425","DOI":"10.1146\/annurev-physiol-031522-084334","article-title":"Polycystin channel complexes","volume":"85","author":"Esarte Palomero","year":"2023","journal-title":"Annu Rev Physiol"},{"key":"2024100315165965800_bib11","first-page":"65","article-title":"Dugesia lugubris s.l. auricles: research into the ultrastructure and on the functional efficiency","volume":"73","author":"Farnesi","year":"1980","journal-title":"Riv Biol"},{"key":"2024100315165965800_bib12","doi-asserted-by":"crossref","first-page":"251","DOI":"10.1016\/j.molmed.2014.01.004","article-title":"Polycystin-1: a master regulator of intersecting cystic pathways","volume":"20","author":"Fedeles","year":"2014","journal-title":"Trends Mol Med"},{"key":"2024100315165965800_bib13","doi-asserted-by":"crossref","first-page":"eaaq1736","DOI":"10.1126\/science.aaq1736","article-title":"Cell type transcriptome atlas for the planarian Schmidtea mediterranea","volume":"360","author":"Fincher","year":"2018","journal-title":"Science"},{"key":"2024100315165965800_bib14","volume-title":"The Orientation of animals","author":"Fraenkel","year":"1961"},{"key":"2024100315165965800_bib15","doi-asserted-by":"crossref","first-page":"2175","DOI":"10.1016\/j.cub.2003.11.053","article-title":"PKD2 cation channel is required for directional sperm movement and male fertility","volume":"13","author":"Gao","year":"2003","journal-title":"Curr Biol"},{"key":"2024100315165965800_bib16","doi-asserted-by":"crossref","first-page":"56","DOI":"10.1038\/nature25473","article-title":"The genome of Schmidtea mediterranea and the evolution of core cellular mechanisms","volume":"554","author":"Grohme","year":"2018","journal-title":"Nature"},{"key":"2024100315165965800_bib17","doi-asserted-by":"crossref","first-page":"321","DOI":"10.1146\/annurev.med.60.101707.125712","article-title":"Polycystic kidney disease","volume":"60","author":"Harris","year":"2009","journal-title":"Annu Rev Med"},{"key":"2024100315165965800_bib18","doi-asserted-by":"crossref","first-page":"e20007","DOI":"10.1371\/journal.pone.0020007","article-title":"Sour taste responses in mice lacking PKD channels","volume":"6","author":"Horio","year":"2011","journal-title":"PLoS One"},{"key":"2024100315165965800_bib19","doi-asserted-by":"crossref","first-page":"754","DOI":"10.1093\/bioinformatics\/17.8.754","article-title":"MRBAYES: Bayesian inference of phylogenetic trees","volume":"17","author":"Huelsenbeck","year":"2001","journal-title":"Bioinformatics"},{"key":"2024100315165965800_bib20","doi-asserted-by":"publisher","DOI":"10.1101\/2023.05.10.540286","article-title":"A wound-induced differentiation trajectory for neurons","author":"Hulett","year":"2023"},{"key":"2024100315165965800_bib21","doi-asserted-by":"crossref","first-page":"e2322864121","DOI":"10.1073\/pnas.2322864121","article-title":"A wound-induced differentiation trajectory for neurons","volume":"121","author":"Hulett","year":"2024","journal-title":"Proc Natl Acad Sci USA"},{"key":"2024100315165965800_bib22","doi-asserted-by":"crossref","first-page":"16","DOI":"10.1111\/dgd.12765","article-title":"Quantification of planarian behaviors","volume":"64","author":"Inoue","year":"2022","journal-title":"Dev Growth Differ"},{"key":"2024100315165965800_bib23","doi-asserted-by":"crossref","first-page":"15701","DOI":"10.1523\/JNEUROSCI.5379-13.2014","article-title":"Thermosensory signaling by TRPM is processed by brain serotonergic neurons to produce planarian thermotaxis","volume":"34","author":"Inoue","year":"2014","journal-title":"J Neurosci"},{"key":"2024100315165965800_bib24","doi-asserted-by":"crossref","first-page":"12569","DOI":"10.1073\/pnas.0602702103","article-title":"Transient receptor potential family members PKD1L3 and PKD2L1 form a candidate sour taste receptor","volume":"103","author":"Ishimaru","year":"2006","journal-title":"Proc Natl Acad Sci USA"},{"key":"2024100315165965800_bib25","doi-asserted-by":"crossref","first-page":"e2321349121","DOI":"10.1073\/pnas.2321349121","article-title":"A niche-derived nonribosomal peptide triggers planarian sexual development","volume":"121","author":"Issigonis","year":"2024","journal-title":"Proc Natl Acad Sci USA"},{"key":"2024100315165965800_bib26","doi-asserted-by":"crossref","first-page":"127","DOI":"10.1016\/bs.ctdb.2019.04.001","article-title":"From worm to germ: germ cell development and regeneration in planarians","volume":"135","author":"Issigonis","year":"2019","journal-title":"Curr Top Dev Biol"},{"key":"2024100315165965800_bib27","doi-asserted-by":"crossref","first-page":"e3001472","DOI":"10.1371\/journal.pbio.3001472","article-title":"A Kruppel-like factor is required for development and regeneration of germline and yolk cells from somatic stem cells in planarians","volume":"20","author":"Issigonis","year":"2022","journal-title":"PLoS Biol"},{"key":"2024100315165965800_bib28","doi-asserted-by":"crossref","first-page":"dev167684","DOI":"10.1242\/dev.167684","article-title":"Model systems for regeneration: planarians","volume":"146","author":"Ivankovic","year":"2019","journal-title":"Development"},{"key":"2024100315165965800_bib29","doi-asserted-by":"crossref","first-page":"385","DOI":"10.1002\/mrd.20042","article-title":"Polycystins: what polycystic kidney disease tells us about sperm","volume":"67","author":"Kierszenbaum","year":"2004","journal-title":"Mol Reprod Dev"},{"key":"2024100315165965800_bib30","doi-asserted-by":"crossref","first-page":"113843","DOI":"10.1016\/j.celrep.2024.113843","article-title":"A transcription factor atlas of stem cell fate in planarians","volume":"43","author":"King","year":"2024","journal-title":"Cell Rep"},{"key":"2024100315165965800_bib31","doi-asserted-by":"crossref","first-page":"8","DOI":"10.1186\/1471-213X-13-8","article-title":"In situ hybridization protocol for enhanced detection of gene expression in the planarian Schmidtea mediterranea","volume":"13","author":"King","year":"2013","journal-title":"BMC Dev Biol"},{"key":"2024100315165965800_bib32","doi-asserted-by":"crossref","first-page":"606","DOI":"10.1007\/BF00338335","article-title":"Beitr\u00e4ge zur Sinnesphysiologie der S\u00fc\u00dfwasserplanarien","volume":"16","author":"Koehler","year":"1932","journal-title":"Z Vergl Physiol"},{"key":"2024100315165965800_bib33","doi-asserted-by":"crossref","first-page":"22","DOI":"10.1016\/j.bbrc.2015.02.067","article-title":"Forcing open TRP channels: mechanical gating as a unifying activation mechanism","volume":"460","author":"Liu","year":"2015","journal-title":"Biochem Biophys Res Commun"},{"key":"2024100315165965800_bib34","doi-asserted-by":"crossref","first-page":"68","DOI":"10.1111\/j.1471-4159.2006.03842.x","article-title":"Two members of the TRPP family of ion channels, Pkd1l3 and Pkd2l1, are co-expressed in a subset of taste receptor cells","volume":"98","author":"LopezJimenez","year":"2006","journal-title":"J Neurochem"},{"key":"2024100315165965800_bib35","doi-asserted-by":"crossref","first-page":"1035507","DOI":"10.3389\/fmolb.2022.1035507","article-title":"The GPCR properties of polycystin-1\u2014a new paradigm","volume":"9","author":"Maser","year":"2022","journal-title":"Front Mol Biosci"},{"key":"2024100315165965800_bib36","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1007\/s10158-016-0194-3","article-title":"Evidence that polycystins are involved in Hydra cnidocyte discharge","volume":"17","author":"McLaughlin","year":"2017","journal-title":"Invert Neurosci"},{"key":"2024100315165965800_bib37","doi-asserted-by":"crossref","first-page":"241","DOI":"10.1007\/978-1-4939-7802-1_5","article-title":"Culturing planarians in the laboratory","volume":"1774","author":"Merryman","year":"2018","journal-title":"Methods Mol Biol"},{"key":"2024100315165965800_bib38","doi-asserted-by":"crossref","first-page":"eaaz0882","DOI":"10.1126\/sciadv.aaz0882","article-title":"The pharyngeal nervous system orchestrates feeding behavior in planarians","volume":"6","author":"Miyamoto","year":"2020","journal-title":"Sci Adv"},{"key":"2024100315165965800_bib39","doi-asserted-by":"crossref","first-page":"1791","DOI":"10.1091\/mbc.E18-04-0270","article-title":"Fission yeast TRP channel Pkd2p localizes to the cleavage furrow and regulates cell separation during cytokinesis","volume":"30","author":"Morris","year":"2019","journal-title":"Mol Biol Cell"},{"key":"2024100315165965800_bib40","doi-asserted-by":"crossref","first-page":"472","DOI":"10.1002\/mrd.20033","article-title":"Polycystin-2 associates with the polycystin-1 homolog, suREJ3, and localizes to the acrosomal region of sea urchin spermatozoa","volume":"67","author":"Neill","year":"2004","journal-title":"Mol Reprod Dev"},{"key":"2024100315165965800_bib41","doi-asserted-by":"crossref","first-page":"25","DOI":"10.1016\/j.semcdb.2014.06.001","article-title":"Mating behavior, male sensory cilia, and polycystins in Caenorhabditis elegans","volume":"33","author":"O'Hagan","year":"2014","journal-title":"Semin Cell Dev Biol"},{"key":"2024100315165965800_bib42","doi-asserted-by":"crossref","first-page":"11153","DOI":"10.1523\/JNEUROSCI.0686-15.2015","article-title":"Mechanosensory genes Pkd1 and Pkd2 contribute to the planar polarization of brain ventricular epithelium","volume":"35","author":"Ohata","year":"2015","journal-title":"J Neurosci"},{"key":"2024100315165965800_bib43","doi-asserted-by":"crossref","first-page":"443","DOI":"10.1002\/dvdy.21849","article-title":"Formaldehyde-based whole-mount in situ hybridization method for planarians","volume":"238","author":"Pearson","year":"2009","journal-title":"Dev Dyn"},{"key":"2024100315165965800_bib44","doi-asserted-by":"crossref","first-page":"eaaq172","DOI":"10.1126\/science.aaq1723","article-title":"Cell type atlas and lineage tree of a whole complex animal by single-cell transcriptomics","volume":"360","author":"Plass","year":"2018","journal-title":"Science"},{"key":"2024100315165965800_bib45","doi-asserted-by":"crossref","first-page":"327","DOI":"10.1016\/j.cell.2018.09.021","article-title":"The cellular and molecular basis for planarian regeneration","volume":"175","author":"Reddien","year":"2018","journal-title":"Cell"},{"key":"2024100315165965800_bib46","doi-asserted-by":"crossref","first-page":"e17002","DOI":"10.7554\/eLife.17002","article-title":"A functional genomics screen in planarians reveals regulators of whole-brain regeneration","volume":"5","author":"Roberts-Galbraith","year":"2016","journal-title":"eLife"},{"key":"2024100315165965800_bib47","doi-asserted-by":"crossref","first-page":"e266","DOI":"10.1002\/wdev.266","article-title":"Nervous system development and regeneration in freshwater planarians","volume":"6","author":"Ross","year":"2017","journal-title":"Wiley Interdiscip Rev Dev Biol"},{"key":"2024100315165965800_bib48","doi-asserted-by":"crossref","first-page":"331","DOI":"10.1016\/j.devcel.2018.10.014","article-title":"SoxB1 activity regulates sensory neuron regeneration, maintenance, and function in planarians","volume":"47","author":"Ross","year":"2018","journal-title":"Dev Cell"},{"key":"2024100315165965800_bib49","doi-asserted-by":"crossref","first-page":"718","DOI":"10.1002\/dvdy.23950","article-title":"RNA interference by feeding in vitro-synthesized double-stranded RNA to planarians: methodology and dynamics","volume":"242","author":"Rouhana","year":"2013","journal-title":"Dev Dyn"},{"key":"2024100315165965800_bib50","doi-asserted-by":"crossref","first-page":"D812","DOI":"10.1093\/nar\/gky1070","article-title":"PlanMine 3.0-improvements to a mineable resource of flatworm biology and biodiversity","volume":"47","author":"Rozanski","year":"2019","journal-title":"Nucleic Acids Res"},{"key":"2024100315165965800_bib51","doi-asserted-by":"crossref","first-page":"e0226104","DOI":"10.1371\/journal.pone.0226104","article-title":"Pharmacological or genetic targeting of transient receptor potential (TRP) channels can disrupt the planarian escape response","volume":"14","author":"Sabry","year":"2019","journal-title":"PLoS One"},{"key":"2024100315165965800_bib52","doi-asserted-by":"crossref","first-page":"299","DOI":"10.1007\/s00441-024-03865-y","article-title":"Structure of putative epidermal sensory receptors in an acoel flatworm, Praesagittifera naikaiensis","volume":"395","author":"Sakagami","year":"2024","journal-title":"Cell Tissue Res"},{"key":"2024100315165965800_bib53","doi-asserted-by":"crossref","first-page":"676","DOI":"10.1038\/nmeth.2019","article-title":"Fiji: an open-source platform for biological-image analysis","volume":"9","author":"Schindelin","year":"2012","journal-title":"Nat Methods"},{"key":"2024100315165965800_bib54","doi-asserted-by":"crossref","first-page":"1520","DOI":"10.1016\/j.cell.2018.05.019","article-title":"Cnidarian cell type diversity and regulation revealed by whole-organism single-cell RNA-seq","volume":"173","author":"Sebe-Pedros","year":"2018","journal-title":"Cell"},{"key":"2024100315165965800_bib55","doi-asserted-by":"crossref","DOI":"10.7554\/eLife.07405","article-title":"Stem cells and fluid flow drive cyst formation in an invertebrate excretory organ","volume":"4","author":"Thi-Kim Vu","year":"2015","journal-title":"eLife"},{"key":"2024100315165965800_bib56","doi-asserted-by":"crossref","first-page":"e31488","DOI":"10.1371\/journal.pone.0031488","article-title":"The Drosophila TRPP cation channel, PKD2 and Dmel\/Ced-12 act in genetically distinct pathways during apoptotic cell clearance","volume":"7","author":"Van Goethem","year":"2012","journal-title":"PLoS One"},{"key":"2024100315165965800_bib57","doi-asserted-by":"crossref","first-page":"D384","DOI":"10.1093\/nar\/gkac1096","article-title":"The conserved domain database in 2023","volume":"51","author":"Wang","year":"2023","journal-title":"Nucleic Acids Res"},{"key":"2024100315165965800_bib58","doi-asserted-by":"crossref","first-page":"2179","DOI":"10.1016\/j.cub.2003.12.002","article-title":"A flagellar polycystin-2 homolog required for male fertility in Drosophila","volume":"13","author":"Watnick","year":"2003","journal-title":"Curr Biol"},{"key":"2024100315165965800_bib59","doi-asserted-by":"crossref","first-page":"632","DOI":"10.1016\/j.devcel.2015.11.004","article-title":"A generic and cell-type-specific wound response precedes regeneration in planarians","volume":"35","author":"Wurtzel","year":"2015","journal-title":"Dev Cell"},{"key":"2024100315165965800_bib60","doi-asserted-by":"crossref","first-page":"101960","DOI":"10.1016\/j.gde.2022.101960","article-title":"Cellular diversity and developmental hierarchy in the planarian nervous system","volume":"76","author":"Wyss","year":"2022","journal-title":"Curr Opin Genet Dev"}],"container-title":["Integrative Organismal Biology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/academic.oup.com\/iob\/advance-article-pdf\/doi\/10.1093\/iob\/obae035\/59080598\/obae035.pdf","content-type":"application\/pdf","content-version":"am","intended-application":"syndication"},{"URL":"https:\/\/academic.oup.com\/iob\/article-pdf\/6\/1\/obae035\/59555848\/obae035.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/academic.oup.com\/iob\/article-pdf\/6\/1\/obae035\/59555848\/obae035.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,10,3]],"date-time":"2024-10-03T11:20:17Z","timestamp":1727954417000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/iob\/article\/doi\/10.1093\/iob\/obae035\/7754887"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024]]},"references-count":60,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2024,1,9]]}},"URL":"https:\/\/doi.org\/10.1093\/iob\/obae035","relation":{"has-preprint":[{"id-type":"doi","id":"10.1101\/2024.07.17.603829","asserted-by":"object"}]},"ISSN":["2517-4843"],"issn-type":[{"value":"2517-4843","type":"electronic"}],"subject":[],"published-other":{"date-parts":[[2024]]},"published":{"date-parts":[[2024]]},"article-number":"obae035"}}