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The physical\/chemical and microbial makeup of WAS is susceptible to seasonal fluctuations, due to the open-air nature of wastewater treatment facilities, potentially impacting subsequent digester performance and the quality of the resulting digestate. This study delved into a comprehensive analysis of both the initial WAS and the digestate produced by 12 full-scale digesters during both a summer and winter sampling campaign. A significant influence of seasonal variations was observed on the physical\/chemical and microbial composition of WAS. Interestingly, the digestate microbiome exhibited a high resilience with minimal seasonal fluctuations, but instead showed variations between different digesters. In summary, this research demonstrates that while WAS composition manifests in specific physical\/chemical attributes, it does not exert a discernible influence on the microbial composition of the resulting digestate.<\/jats:p>","DOI":"10.1093\/ismeco\/ycaf072","type":"journal-article","created":{"date-parts":[[2025,4,25]],"date-time":"2025-04-25T07:46:41Z","timestamp":1745567201000},"source":"Crossref","is-referenced-by-count":0,"title":["The anaerobic digestion microbiome is robust toward variation in the waste activated sludge feed"],"prefix":"10.1093","volume":"6","author":[{"given":"Josefien","family":"Van Landuyt","sequence":"first","affiliation":[{"name":"Centre for Microbial Ecology and Technology, Universiteit Gent , Frieda Saeysstraat 1, Gent B-9052 ,","place":["Belgium"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jasmine","family":"Oosterlinck","sequence":"additional","affiliation":[{"name":"Centre for Microbial Ecology and Technology, Universiteit Gent , Frieda Saeysstraat 1, Gent B-9052 ,","place":["Belgium"]},{"name":"Witteveen+Bos Belgium N.V. , Posthoflei 5, Bus 1, 2600 Antwerpen\u2014Berchem ,","place":["Belgium"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jo","family":"De Vrieze","sequence":"additional","affiliation":[{"name":"Centre for Microbial Ecology and Technology, Universiteit Gent , Frieda Saeysstraat 1, Gent B-9052 ,","place":["Belgium"]},{"name":"Centre for Advanced Process Technology for Urban Resource Recovery , Frieda Saeysstraat 1, Gent B-9052 ,","place":["Belgium"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2025,4,25]]},"reference":[{"key":"2026060817375899400_ref1","doi-asserted-by":"publisher","first-page":"122139","DOI":"10.1016\/j.jhazmat.2020.122139","article-title":"Paradigm shifts and current challenges in wastewater management","volume":"390","author":"Villar\u00edn","year":"2020","journal-title":"J Hazard 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