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However, the spatial behavior of PPFMs on plant surfaces and its molecular basis are unknown. Here, we show that Methylobacterium sp. strain OR01 inoculated onto red perilla seeds colonized across the entire plant surface in the phyllosphere concomitant with the plant growth. During its transmission, strain OR01 was found to be present on the entire leaf surface with a preference to sites around the periphery, vein, trichome, and stomata. We found that methanol-sensing chemoreceptor MtpA-dependent chemotaxis (methylotaxis; chemotaxis toward methanol) and flagellin protein FliC-dependent motility facilitated the bacterial entry into the stomatal cavity and their colonization in the phyllosphere.<\/jats:p>","DOI":"10.1093\/ismeco\/ycaf092","type":"journal-article","created":{"date-parts":[[2025,5,28]],"date-time":"2025-05-28T07:47:55Z","timestamp":1748418475000},"source":"Crossref","is-referenced-by-count":10,"title":["Methanol chemoreceptor MtpA- and flagellin protein FliC-dependent methylotaxis contributes to the spatial colonization of PPFM in the phyllosphere"],"prefix":"10.1093","volume":"5","author":[{"given":"Shiori","family":"Katayama","sequence":"first","affiliation":[{"name":"Graduate School of Agriculture, Kyoto University, Kitashirakawa-Oiwake, Sakyo-ku , Kyoto 606-8502 ,","place":["Japan"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kosuke","family":"Shiraishi","sequence":"additional","affiliation":[{"name":"Graduate School of Agriculture, Kyoto University, Kitashirakawa-Oiwake, Sakyo-ku , Kyoto 606-8502 ,","place":["Japan"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kanae","family":"Kaji","sequence":"additional","affiliation":[{"name":"Graduate School of Agriculture, Kyoto University, Kitashirakawa-Oiwake, Sakyo-ku , Kyoto 606-8502 ,","place":["Japan"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kazuya","family":"Kawabata","sequence":"additional","affiliation":[{"name":"Graduate School of Agriculture, Kyoto University, Kitashirakawa-Oiwake, Sakyo-ku , Kyoto 606-8502 ,","place":["Japan"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Naoki","family":"Tamura","sequence":"additional","affiliation":[{"name":"Department of Anatomy and Histology, School of Medicine, Fukushima Medical University , Hikarigaoka 1, Fukushima 960-1295 ,","place":["Japan"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Akio","family":"Tani","sequence":"additional","affiliation":[{"name":"Institute of Plant Science and Resources, Okayama University , 2-20-1, Chuo, Kurashiki, Okayama 710-0046 ,","place":["Japan"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hiroya","family":"Yurimoto","sequence":"additional","affiliation":[{"name":"Graduate School of Agriculture, Kyoto University, Kitashirakawa-Oiwake, Sakyo-ku , Kyoto 606-8502 ,","place":["Japan"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yasuyoshi","family":"Sakai","sequence":"additional","affiliation":[{"name":"Graduate School of Agriculture, Kyoto University, Kitashirakawa-Oiwake, Sakyo-ku , Kyoto 606-8502 ,","place":["Japan"]},{"name":"Graduate School of Advanced Integrated Studies in Human Survivability, Kyoto University , Yoshida Nakaadachi-Cho 1, Sakyo-ku, Kyoto 606-8306 ,","place":["Japan"]},{"name":"Department of Bioscience and Biotechnology, Faculty of Bioenvironmental Sciences, Kyoto University of Advanced Science, 1-1 Sogabe-cho Nanjo Otani , Kameoka, Kyoto 621-0023 ,","place":["Japan"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2025,5,29]]},"reference":[{"key":"2025062703350710600_ref1","doi-asserted-by":"publisher","first-page":"16428","DOI":"10.1073\/pnas.0905240106","article-title":"Community proteogenomics reveals insights into the physiology of phyllosphere bacteria","volume":"106","author":"Delmotte","year":"2009","journal-title":"Proc Natl Acad Sci USA"},{"key":"2025062703350710600_ref2","doi-asserted-by":"publisher","first-page":"92","DOI":"10.1016\/j.micres.2006.10.007","article-title":"Intercellular colonization and growth promoting effects of Methylobacterium sp. with plant-growth regulators on rice (Oryza sativa l. cv 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