{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,23]],"date-time":"2026-10-23T20:05:55Z","timestamp":1792785955326,"version":"4.5.0"},"reference-count":51,"publisher":"Oxford University Press (OUP)","issue":"1","license":[{"start":{"date-parts":[[2026,7,12]],"date-time":"2026-07-12T00:00:00Z","timestamp":1783814400000},"content-version":"vor","delay-in-days":192,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Beijing Municipal Science & Technology Project, China","award":["Z241100007724009"],"award-info":[{"award-number":["Z241100007724009"]}]},{"name":"Biological Resources Programme, Chinese Academy of Sciences","award":["CAS-TAX-24-024"],"award-info":[{"award-number":["CAS-TAX-24-024"]}],"id":[{"id":"https:\/\/ror.org\/034t30j35","id-type":"ROR","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["32170007"],"award-info":[{"award-number":["32170007"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Strategic Priority Research Program of the Chinese Academy of Sciences","award":["XDB0810000"],"award-info":[{"award-number":["XDB0810000"]}],"id":[{"id":"https:\/\/ror.org\/034t30j35","id-type":"ROR","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026,1,14]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Accelerating glacier retreat exports cryospheric microbiomes into downstream ecosystems, raising questions about their functional roles. However, a persistent gap between genomic predictions and physiologically validated functions exists, largely due to the scarcity of cultivated and taxonomically resolved model organisms from these environments. Here we characterize 23 Pseudomonas strains isolated from ice, cryoconite, and supraglacial meltwater collected across ten glaciers in China. Phylogenomic inference places them within the Pseudomonas mandelii subgroup, and genome metrics delineate 22 strains as distinct novel species (ANI \u226495.71%; dDDH \u226464.6% to their closest type strains). Phenotypic analyses showed broad environmental tolerance, including growth at \u22122\u00b0C, strain-dependent upper growth limits of 30\u00b0C\u201337\u00b0C, and growth over an approximate pH range of 5.0\u201311.0. Genome-based reconstruction predicted heterogeneous nitrogen-transforming potential, including DNRA-related genes in all strains, nitrate-reduction genes in 14 strains, complete denitrification marker sets in 12 strains, and nitrogen-fixation genes in one strain. Phenotypic assays showed broader nitrate-reduction activity than predicted, with all 23 strains reducing nitrate to nitrite and seven showing reduction beyond nitrite, underscoring the value of cultivation-based functional validation in non-model cryospheric bacteria. Comparative proteomics of four representative species at 0\u00b0C versus 28\u00b0C reveals shared temperature-responsive pathways and a common network backbone involving translation, proteostasis, and carbon economy. Together, these cultured genomes provide valuable models for linking cryospheric Pseudomonas diversity to temperature-responsive physiology and biogeochemical potential.<\/jats:p>","DOI":"10.1093\/ismeco\/ycag204","type":"journal-article","created":{"date-parts":[[2026,7,10]],"date-time":"2026-07-10T11:57:14Z","timestamp":1783684634000},"source":"Crossref","is-referenced-by-count":0,"title":["Twenty-two novel glacier\n                    <i>Pseudomonas<\/i>\n                    species expand cultivated representation: nitrogen-cycling potential and temperature-responsive proteomes"],"prefix":"10.1093","volume":"6","author":[{"given":"Chan","family":"Zhao","sequence":"first","affiliation":[{"name":"State Key Laboratory of Microbial Diversity and Innovative Utilization, Institute of Microbiology, Chinese Academy of Sciences , Beijing 100101 ,","place":["China"]},{"name":"State Key Laboratory of Animal Biotech Breeding and College of Biological Sciences, China Agricultural University , Beijing 100193 ,","place":["P. R. China"]},{"name":"China General Microbiological Culture Collection Center (CGMCC), Institute of Microbiology, Chinese Academy of Sciences , Beijing 100101 ,","place":["P. R. China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lei-Lei","family":"Yang","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Microbial Diversity and Innovative Utilization, Institute of Microbiology, Chinese Academy of Sciences , Beijing 100101 ,","place":["China"]},{"name":"China General Microbiological Culture Collection Center (CGMCC), Institute of Microbiology, Chinese Academy of Sciences , Beijing 100101 ,","place":["P. R. China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yu-Hua","family":"Xin","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Microbial Diversity and Innovative Utilization, Institute of Microbiology, Chinese Academy of Sciences , Beijing 100101 ,","place":["China"]},{"name":"China General Microbiological Culture Collection Center (CGMCC), Institute of Microbiology, Chinese Academy of Sciences , Beijing 100101 ,","place":["P. R. China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2700-9956","authenticated-orcid":false,"given":"Qing","family":"Liu","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Microbial Diversity and Innovative Utilization, Institute of Microbiology, Chinese Academy of Sciences , Beijing 100101 ,","place":["China"]},{"name":"China General Microbiological Culture Collection Center (CGMCC), Institute of Microbiology, Chinese Academy of Sciences , Beijing 100101 ,","place":["P. R. China"]},{"name":"Beijing Key Laboratory of Genetic Element Biosourcing & Intelligent Design for Biomanufacturing , Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101 ,","place":["P. R. China"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2026,7,12]]},"reference":[{"key":"2026092314090699700_ref1","doi-asserted-by":"publisher","first-page":"219","DOI":"10.1016\/j.tree.2011.09.012","article-title":"Glaciers and ice sheets as a biome","volume":"27","author":"Anesio","year":"2012","journal-title":"Trends Ecol Evol"},{"key":"2026092314090699700_ref2","doi-asserted-by":"publisher","first-page":"346","DOI":"10.1016\/j.resmic.2010.12.004","article-title":"Diversity and ecology of psychrophilic microorganisms","volume":"162","author":"Margesin","year":"2011","journal-title":"Res Microbiol"},{"key":"2026092314090699700_ref3","doi-asserted-by":"publisher","first-page":"578","DOI":"10.1016\/j.syapm.2015.09.005","article-title":"High diversity and distinctive community structure of bacteria on glaciers in China revealed by 454 pyrosequencing","volume":"38","author":"Liu","year":"2015","journal-title":"Syst Appl 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