{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,28]],"date-time":"2026-09-28T13:00:02Z","timestamp":1790600402230,"version":"4.1.0"},"reference-count":120,"publisher":"Oxford University Press (OUP)","issue":"1","license":[{"start":{"date-parts":[[2026,9,18]],"date-time":"2026-09-18T00:00:00Z","timestamp":1789689600000},"content-version":"vor","delay-in-days":260,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000001","name":"National Science Foundation","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100000001","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000268","name":"Biotechnology and Biological Sciences Research Council","doi-asserted-by":"publisher","award":["IOS-2019368"],"award-info":[{"award-number":["IOS-2019368"]}],"id":[{"id":"10.13039\/501100000268","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000268","name":"Biotechnology and Biological Sciences Research Council","doi-asserted-by":"publisher","award":["BB\/V011278\/1"],"award-info":[{"award-number":["BB\/V011278\/1"]}],"id":[{"id":"10.13039\/501100000268","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["R21AI138074"],"award-info":[{"award-number":["R21AI138074"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000001","name":"NSF","doi-asserted-by":"publisher","award":["DGE-2137424"],"award-info":[{"award-number":["DGE-2137424"]}],"id":[{"id":"10.13039\/100000001","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026,8,5]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Mosquitoes are important vectors of human pathogens, and their gut microbiome influences traits such as survival, fecundity, pathogen resistance, insecticide resistance, and vector competence. Manipulating this microbiome offers a promising strategy for reducing mosquito-borne disease. While 16S rRNA gene amplicon sequencing has revealed global patterns of gut microbial diversity, the factors driving these patterns remain poorly understood. Here, we used a community-transplantation approach that allows the collection, cryopreservation, and transfer of whole adult microbial communities into axenic (germ-free) mosquitoes to investigate the determinants of microbiome assembly and host\u2013microbe interactions. Using reciprocal transplants between Aedes aegypti Liverpool strains maintained in isolation for nearly 50\u00a0years at the University of Wisconsin-Madison (UW) and the Liverpool School of Tropical Medicine (LSTM), we found striking strain-specific responses: UW mosquitoes exhibited altered bacterial composition and developmental defects, whereas LSTM mosquitoes did not. Transplant fidelity depended on donor community composition and recipient genotype, and we observed conserved assembly patterns across the mosquito life cycle. These results reveal how host genetics and microbial community structure interact to shape microbiome assembly and support the development of microbiome-based vector control strategies.<\/jats:p>","DOI":"10.1093\/ismehm\/aazag010","type":"journal-article","created":{"date-parts":[[2026,9,18]],"date-time":"2026-09-18T15:34:27Z","timestamp":1789745667000},"source":"Crossref","is-referenced-by-count":0,"title":["Transplant of cryopreserved mosquito gut microbiota reveals strain-specific patterns of microbial acquisition and assembly"],"prefix":"10.1093","volume":"1","author":[{"given":"Holly L","family":"Nichols","sequence":"first","affiliation":[{"name":"Department of Bacteriology, University of Wisconsin-Madison , Madison, WI 53706 ,","place":["United States"]},{"name":"Microbiology Doctoral Training Program, University of Wisconsin-Madison , Madison, WI 53706 ,","place":["United States"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vishaal","family":"Dhokiya","sequence":"additional","affiliation":[{"name":"Departments of Vector Biology and Tropical Disease Biology, Centre for Neglected Tropical Diseases, Liverpool School of Tropical Medicine , Liverpool L3 5QA ,","place":["United Kingdom"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ananya F","family":"Hoque","sequence":"additional","affiliation":[{"name":"Departments of Vector Biology and Tropical Disease Biology, Centre for Neglected Tropical Diseases, Liverpool School of Tropical Medicine , Liverpool L3 5QA ,","place":["United Kingdom"]},{"name":"The Roslin Institute, Royal (Dick) School of Veterinary Studies, University of Edinburgh , Edinburgh EH25 9RG ,","place":["United Kingdom"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eva","family":"Heinz","sequence":"additional","affiliation":[{"name":"Strathclyde Institute of Pharmacy and Biomedical Sciences, University of Strathclyde , Glasgow G1 1XQ ,","place":["United Kingdom"]},{"name":"Departments of Clinical Sciences and Vector Biology, Liverpool School of Tropical Medicine , Liverpool ,","place":["United Kingdom"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Grant L","family":"Hughes","sequence":"additional","affiliation":[{"name":"Departments of Vector Biology and Tropical Disease Biology, Centre for Neglected Tropical Diseases, Liverpool School of Tropical Medicine , Liverpool L3 5QA ,","place":["United Kingdom"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2701-5195","authenticated-orcid":false,"given":"Kerri L","family":"Coon","sequence":"additional","affiliation":[{"name":"Department of Bacteriology, University of Wisconsin-Madison , Madison, WI 53706 ,","place":["United States"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2026,9,18]]},"reference":[{"key":"2026092808255251000_ref1","volume-title":"Vector-Borne Diseases","author":"World Health Organization","year":"2024"},{"key":"2026092808255251000_ref2","doi-asserted-by":"publisher","first-page":"320","DOI":"10.1079\/9781800621176.0016","article-title":"Paratransgenesis in mosquitoes and other insects: microbial ecology and bacterial genetic considerations","author":"Guido","year":"2022","journal-title":"Transgenic Insects"},{"key":"2026092808255251000_ref3","doi-asserted-by":"publisher","first-page":"98","DOI":"10.1016\/j.pt.2019.12.001","article-title":"Mosquito microbiota and implications for disease control","volume":"36","author":"Gao","year":"2020","journal-title":"Trends Parasitol"},{"key":"2026092808255251000_ref4","doi-asserted-by":"publisher","first-page":"165","DOI":"10.1007\/s10682-022-10197-2","article-title":"Beyond canonical models: why a broader understanding of Diptera-microbiota interactions is essential for vector-borne disease control","volume":"37","author":"Arellano","year":"2023","journal-title":"Evol Ecol"},{"key":"2026092808255251000_ref5","doi-asserted-by":"publisher","first-page":"670","DOI":"10.1016\/j.pt.2025.06.010","article-title":"Leveraging microbial ecology for mosquito-borne disease control","volume":"41","author":"Nichols","year":"2025","journal-title":"Trends Parasitol"},{"key":"2026092808255251000_ref6","doi-asserted-by":"publisher","first-page":"e1013431","DOI":"10.1371\/journal.ppat.1013431","article-title":"Emerging challenges for mosquito-borne disease control and the promise of symbiont-based transmission-blocking strategies","volume":"21","author":"Gao","year":"2025","journal-title":"PLoS Pathog"},{"key":"2026092808255251000_ref7","doi-asserted-by":"publisher","first-page":"111","DOI":"10.1186\/s40168-021-01073-2","article-title":"The microbiome and mosquito vectorial capacity: rich potential for discovery and translation","volume":"9","author":"Cansado-Utrilla","year":"2021","journal-title":"Microbiome."},{"key":"2026092808255251000_ref8","doi-asserted-by":"publisher","first-page":"e2101080118","DOI":"10.1073\/pnas.2101080118","article-title":"Riboflavin instability is a key factor underlying the requirement of a gut microbiota for mosquito development","volume":"118","author":"Wang","year":"2021","journal-title":"Proc Natl Acad Sci U S A"},{"key":"2026092808255251000_ref9","doi-asserted-by":"publisher","first-page":"942","DOI":"10.1038\/s41467-021-21195-3","article-title":"Production of germ-free mosquitoes via transient colonisation allows stage-specific investigation of host\u2013microbiota interactions","volume":"12","author":"Romoli","year":"2021","journal-title":"Nat Commun"},{"key":"2026092808255251000_ref10","doi-asserted-by":"publisher","first-page":"2727","DOI":"10.1111\/mec.12771","article-title":"Mosquitoes rely on their gut microbiota for development","volume":"23","author":"Coon","year":"2014","journal-title":"Mol Ecol"},{"key":"2026092808255251000_ref11","doi-asserted-by":"publisher","first-page":"e0006638","DOI":"10.1371\/journal.pntd.0006638","article-title":"Both living bacteria and eukaryotes in the mosquito gut promote growth of larvae","volume":"12","author":"Valzania","year":"2018","journal-title":"PLoS Negl Trop Dis"},{"key":"2026092808255251000_ref12","doi-asserted-by":"publisher","first-page":"632","DOI":"10.1093\/jme\/tjz003","article-title":"A diverse microbial community supports larval development and survivorship of the Asian Tiger mosquito (Diptera: Culicidae)","volume":"56","author":"Travanty","year":"2019","journal-title":"J Med Entomol"},{"key":"2026092808255251000_ref13","doi-asserted-by":"publisher","first-page":"e0005034","DOI":"10.1371\/journal.pntd.0005034","article-title":"Polyphenol-rich diets exacerbate AMPK-mediated autophagy, decreasing proliferation of mosquito midgut microbiota, and extending vector lifespan","volume":"10","author":"Nunes","year":"2016","journal-title":"PLoS Negl Trop Dis"},{"key":"2026092808255251000_ref14","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1038\/s42003-023-05545-z","article-title":"The mosquito Aedes aegypti requires a gut microbiota for normal fecundity, longevity and vector competence","volume":"6","author":"Harrison","year":"2023","journal-title":"Commun Biol"},{"key":"2026092808255251000_ref15","doi-asserted-by":"publisher","first-page":"375","DOI":"10.1186\/s13071-016-1660-9","article-title":"Gut bacteria differentially affect egg production in the anautogenous mosquito Aedes aegypti and facultatively autogenous mosquito Aedes atropalpus (Diptera: Culicidae)","volume":"9","author":"Coon","year":"2016","journal-title":"Parasit Vectors"},{"key":"2026092808255251000_ref16","doi-asserted-by":"publisher","first-page":"e1700585","DOI":"10.1126\/sciadv.1700585","article-title":"Carryover effects of larval exposure to different environmental bacteria drive adult trait variation in a mosquito vector","volume":"3","author":"Dickson","year":"2017","journal-title":"Sci Adv"},{"key":"2026092808255251000_ref17","doi-asserted-by":"publisher","first-page":"8221","DOI":"10.1038\/s41467-024-52566-1","article-title":"Gut symbiont-derived sphingosine modulates vector competence in Aedes mosquitoes","volume":"15","author":"Sun","year":"2024","journal-title":"Nat Commun"},{"key":"2026092808255251000_ref18","doi-asserted-by":"publisher","first-page":"533","DOI":"10.1126\/science.adf8141","article-title":"Delftia tsuruhatensis TC1 symbiont suppresses malaria transmission by Anopheline mosquitoes","volume":"381","author":"Huang","year":"2023","journal-title":"Science."},{"key":"2026092808255251000_ref19","doi-asserted-by":"publisher","first-page":"116","DOI":"10.1186\/s42523-025-00482-0","article-title":"Mosquito host background impacts microbiome-Zika virus interactions in field- and laboratory-reared Aedes aegypti","volume":"7","author":"Cansado-Utrilla","year":"2025","journal-title":"Anim Microbiome"},{"key":"2026092808255251000_ref20","doi-asserted-by":"publisher","first-page":"2084","DOI":"10.1038\/s41598-018-20367-4","article-title":"Whole metagenome sequencing reveals links between mosquito microbiota and insecticide resistance in malaria vectors","volume":"8","author":"Dada","year":"2018","journal-title":"Sci Rep"},{"key":"2026092808255251000_ref21","doi-asserted-by":"crossref","first-page":"e0010208","DOI":"10.1371\/journal.pntd.0010208","article-title":"A symbiotic gut bacterium enhances Aedes albopictus resistance to insecticide","volume":"16","author":"Wang","year":"2022","journal-title":"PLoS Negl Trop Dis"},{"key":"2026092808255251000_ref22","doi-asserted-by":"publisher","first-page":"9117","DOI":"10.1038\/s41598-019-45499-z","article-title":"The contribution of gut bacteria to insecticide resistance and the life histories of the major malaria vector Anopheles arabiensis (Diptera: Culicidae)","volume":"9","author":"Barnard","year":"2019","journal-title":"Sci Rep"},{"key":"2026092808255251000_ref23","doi-asserted-by":"publisher","first-page":"645362","DOI":"10.3389\/fmicb.2021.645362","article-title":"Larval diet abundance influences size and composition of the midgut microbiota of Aedes aegypti mosquitoes","volume":"12","author":"MacLeod","year":"2021","journal-title":"Front Microbiol"},{"key":"2026092808255251000_ref24","doi-asserted-by":"publisher","first-page":"e00543","DOI":"10.1128\/msphere.00543-25","article-title":"Contrasted impacts of commercial diets and rearing water on Aedes aegypti fitness and microbiota","volume":"0","author":"Calvez","year":"2025","journal-title":"mSphere."},{"key":"2026092808255251000_ref25","doi-asserted-by":"publisher","first-page":"83","DOI":"10.1186\/s13071-021-04579-8","article-title":"Blood meal source and mixed blood-feeding influence gut bacterial community composition in Aedes aegypti","volume":"14","author":"Muturi","year":"2021","journal-title":"Parasit Vectors"},{"key":"2026092808255251000_ref26","doi-asserted-by":"publisher","first-page":"332","DOI":"10.3390\/microorganisms10020332","article-title":"Influence of host blood meal source on gut microbiota of wild caught Aedes aegypti, a dominant arboviral disease vector","volume":"10","author":"Sarma","year":"2022","journal-title":"Microorganisms."},{"key":"2026092808255251000_ref27","doi-asserted-by":"publisher","first-page":"650743","DOI":"10.3389\/fmicb.2021.650743","article-title":"Diet\u2013microbiota interactions alter mosquito development","volume":"12","author":"Martinson","year":"2021","journal-title":"Front Microbiol"},{"key":"2026092808255251000_ref28","doi-asserted-by":"publisher","first-page":"857","DOI":"10.1111\/een.13360","article-title":"Causes and consequences of microbiome formation in mosquito larvae","volume":"49","author":"Schwing","year":"2024","journal-title":"Ecol Entomol"},{"key":"2026092808255251000_ref29","doi-asserted-by":"publisher","first-page":"59","DOI":"10.1016\/j.cois.2018.05.008","article-title":"Composition and functional roles of the gut microbiota in mosquitoes","volume":"28","author":"Strand","year":"2018","journal-title":"Curr Opin Insect Sci"},{"key":"2026092808255251000_ref30","doi-asserted-by":"publisher","first-page":"e24767","DOI":"10.1371\/journal.pone.0024767","article-title":"Dynamic gut microbiome across life history of the malaria mosquito Anopheles gambiae in Kenya","volume":"6","author":"Wang","year":"2011","journal-title":"PLoS One"},{"key":"2026092808255251000_ref31","doi-asserted-by":"publisher","first-page":"2832","DOI":"10.3389\/fmicb.2019.02832","article-title":"Changes in microbiota across developmental stages of Aedes koreicus, an invasive mosquito vector in Europe: indications for microbiota-based control strategies","volume":"10","author":"Alfano","year":"2019","journal-title":"Front Microbiol"},{"key":"2026092808255251000_ref32","doi-asserted-by":"publisher","first-page":"e0194899","DOI":"10.1371\/journal.pone.0194899","article-title":"Investigation of the seasonal microbiome of Anopheles coluzzii mosquitoes in Mali","volume":"13","author":"Krajacich","year":"2018","journal-title":"PLoS One"},{"key":"2026092808255251000_ref33","doi-asserted-by":"publisher","first-page":"wrae181","DOI":"10.1093\/ismejo\/wrae181","article-title":"Abiotic factors shape mosquito microbiomes that enhance host development","volume":"18","author":"Kriefall","year":"2024","journal-title":"ISME J"},{"key":"2026092808255251000_ref34","doi-asserted-by":"publisher","first-page":"893","DOI":"10.1007\/s00248-021-01865-x","article-title":"Mosquito microbiome diversity varies along a landscape-scale moisture gradient","volume":"84","author":"Medeiros","year":"2022","journal-title":"Microb Ecol"},{"key":"2026092808255251000_ref35","doi-asserted-by":"publisher","first-page":"103199","DOI":"10.1016\/j.parint.2025.103199","article-title":"Microbiome and physicochemical properties of breeding waters of Aedes albopictus mosquitoes in Sri Lanka","volume":"111","author":"Wijesundara","year":"2026","journal-title":"Parasitol Int"},{"key":"2026092808255251000_ref36","doi-asserted-by":"publisher","first-page":"551","DOI":"10.1038\/s41579-025-01164-z","article-title":"Origin and function of beneficial bacterial symbioses in insects","volume":"23","author":"Kaltenpoth","year":"2025","journal-title":"Nat Rev Microbiol"},{"key":"2026092808255251000_ref37","doi-asserted-by":"publisher","first-page":"R485","DOI":"10.1016\/j.cub.2019.03.032","article-title":"The life of an insect endosymbiont from the cradle to the grave","volume":"29","author":"McCutcheon","year":"2019","journal-title":"Curr Biol"},{"key":"2026092808255251000_ref38","doi-asserted-by":"publisher","first-page":"10104","DOI":"10.1038\/s41467-025-65129-9","article-title":"Genome degradation results in nested symbiosis and endosymbiont replacement in cicadas","volume":"16","author":"Zhou","year":"2025","journal-title":"Nat Commun"},{"key":"2026092808255251000_ref39","doi-asserted-by":"publisher","first-page":"100882","DOI":"10.1016\/j.cois.2022.100882","article-title":"Aphid hologenomics: current status and future challenges","volume":"50","author":"Shigenobu","year":"2022","journal-title":"Curr Opin Insect Sci"},{"key":"2026092808255251000_ref40","doi-asserted-by":"publisher","DOI":"10.1128\/mbio.02164-15","article-title":"The bee microbiome: impact on bee health and model for evolution and ecology of host-microbe interactions","author":"Engel","journal-title":"mBio."},{"key":"2026092808255251000_ref41","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-ento-121423-013446","article-title":"Transient microbes in insects: fleeting but functional","author":"Ravenscraft","journal-title":"Annu Rev Entomol"},{"key":"2026092808255251000_ref42","doi-asserted-by":"publisher","first-page":"4308","DOI":"10.1128\/AEM.00067-07","article-title":"Insect-microbe mutualism without vertical transmission: a stinkbug acquires a beneficial gut symbiont from the environment every generation","volume":"73","author":"Kikuchi","year":"2007","journal-title":"Appl Environ Microbiol"},{"key":"2026092808255251000_ref43","doi-asserted-by":"publisher","first-page":"446","DOI":"10.1038\/ismej.2010.150","article-title":"An ancient but promiscuous host\u2013symbiont association between Burkholderia gut symbionts and their heteropteran hosts","volume":"5","author":"Kikuchi","year":"2011","journal-title":"ISME J"},{"key":"2026092808255251000_ref44","doi-asserted-by":"publisher","first-page":"369","DOI":"10.1093\/icb\/42.2.369","article-title":"Partner choice in nitrogen-fixation mutualisms of legumes and rhizobia","volume":"42","author":"Simms","year":"2002","journal-title":"Integr Comp Biol"},{"key":"2026092808255251000_ref45","doi-asserted-by":"publisher","first-page":"666","DOI":"10.1038\/s41579-021-00567-y","article-title":"A lasting symbiosis: how the Hawaiian bobtail squid finds and keeps its bioluminescent bacterial partner","volume":"19","author":"Nyholm","year":"2021","journal-title":"Nat Rev Microbiol"},{"key":"2026092808255251000_ref46","doi-asserted-by":"publisher","first-page":"5","DOI":"10.1093\/jisesa\/ieae100","article-title":"Effects of the symbiotic bacteria, Caballeronia insecticola, on the life history parameters of Riptortus pedestris (Hemiptera: Alydidae) and their implications for the host population growth","volume":"24","author":"Kho","year":"2024","journal-title":"J Insect Sci"},{"key":"2026092808255251000_ref47","doi-asserted-by":"publisher","first-page":"166","DOI":"10.1093\/evolut\/qpac009","article-title":"Evaluating coevolution in a horizontally transmitted mutualism","volume":"77","author":"Stoy","year":"2023","journal-title":"Evolution."},{"key":"2026092808255251000_ref48","doi-asserted-by":"publisher","first-page":"17789","DOI":"10.1038\/s41598-024-68753-5","article-title":"Selection for stress tolerance and longevity in Drosophila melanogaster have strong impacts on microbiome profiles","volume":"14","author":"Kristensen","year":"2024","journal-title":"Sci Rep"},{"key":"2026092808255251000_ref49","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.01401-20","article-title":"Gut bacteria mediate nutrient availability in Drosophila diets","author":"Lesperance","journal-title":"Appl Environ Microbiol"},{"key":"2026092808255251000_ref50","doi-asserted-by":"publisher","first-page":"2637","DOI":"10.1111\/j.1365-294X.2005.02615.x","article-title":"Enterobacteria-mediated nitrogen fixation in natural populations of the fruit fly Ceratitis capitata","volume":"14","author":"Behar","year":"2005","journal-title":"Mol Ecol"},{"key":"2026092808255251000_ref51","doi-asserted-by":"publisher","first-page":"1745","DOI":"10.1111\/jeb.14213","article-title":"Alteration of diet microbiota limits the experimentally evolved immune priming response in flour beetles, but not pathogen resistance","volume":"36","author":"Prakash","year":"2023","journal-title":"J Evol Biol"},{"key":"2026092808255251000_ref52","doi-asserted-by":"publisher","first-page":"66","DOI":"10.1016\/j.chembiol.2016.11.015","article-title":"Symbiont-derived antimicrobials contribute to the control of the lepidopteran gut microbiota","volume":"24","author":"Shao","year":"2017","journal-title":"Cell Chem Biol"},{"key":"2026092808255251000_ref53","doi-asserted-by":"publisher","first-page":"117","DOI":"10.1146\/annurev-ento-020723-102548","article-title":"Toward an integrated understanding of the Lepidoptera microbiome","volume":"69","author":"Shao","year":"2024","journal-title":"Annu Rev Entomol"},{"key":"2026092808255251000_ref54","doi-asserted-by":"publisher","first-page":"e0229848","DOI":"10.1371\/journal.pone.0229848","article-title":"Diet influences proliferation and stability of gut bacterial populations in herbivorous lepidopteran larvae","volume":"15","author":"Mason","year":"2020","journal-title":"PLoS One"},{"key":"2026092808255251000_ref55","doi-asserted-by":"publisher","first-page":"4006","DOI":"10.1073\/pnas.1722232115","article-title":"Processes and patterns of interaction as units of selection: an introduction to ITSNTS thinking","volume":"115","author":"Doolittle","year":"2018","journal-title":"Proc Natl Acad Sci U S A"},{"key":"2026092808255251000_ref56","doi-asserted-by":"publisher","first-page":"e00778","DOI":"10.1128\/aem.00778-23","article-title":"Three species of axenic mosquito larvae recruit a shared core of bacteria in a common garden experiment","volume":"89","author":"Hyde","year":"2023","journal-title":"Appl Environ Microbiol"},{"key":"2026092808255251000_ref57","doi-asserted-by":"publisher","first-page":"3352","DOI":"10.1038\/s41598-020-60075-6","article-title":"The environment and species affect gut bacteria composition in laboratory co-cultured Anopheles gambiae and Aedes albopictus mosquitoes","volume":"10","author":"Saab","year":"2020","journal-title":"Sci Rep"},{"key":"2026092808255251000_ref58","doi-asserted-by":"publisher","first-page":"20192834","DOI":"10.1098\/rspb.2019.2834","article-title":"Into the wild: microbiome transplant studies need broader ecological reality","volume":"287","author":"Greyson-Gaito","year":"2020","journal-title":"Proc Biol Sci"},{"key":"2026092808255251000_ref59","first-page":"854","article-title":"Fecal enema as an adjunct in the treatment of Pseudomembranous enterocolitis","volume":"44","author":"Eiseman","year":"1958","journal-title":"Surgery."},{"key":"2026092808255251000_ref60","doi-asserted-by":"publisher","first-page":"239","DOI":"10.1007\/s00335-021-09863-7","article-title":"The gut microbiome of laboratory mice: considerations and best practices for translational research","volume":"32","author":"Ericsson","year":"2021","journal-title":"Mamm Genome"},{"key":"2026092808255251000_ref61","doi-asserted-by":"publisher","first-page":"2050312117708712","DOI":"10.1177\/2050312117708712","article-title":"Faecal microbiota transplantation: where did it start? What have studies taught us? Where is it going?","volume":"5","author":"Chanyi","year":"2017","journal-title":"SAGE Open Med"},{"key":"2026092808255251000_ref62","doi-asserted-by":"publisher","first-page":"10.1128\/msystems.00088-17","DOI":"10.1128\/msystems.00088-17","article-title":"The induction of oxalate metabolism in vivo is more effective with functional microbial communities than with functional microbial species","volume":"2","author":"Miller","year":"2017","journal-title":"mSystems."},{"key":"2026092808255251000_ref63","doi-asserted-by":"publisher","first-page":"1720","DOI":"10.1111\/1462-2920.12841","article-title":"Inoculation of tannin-degrading bacteria into novel hosts increases performance on tannin-rich diets","volume":"18","author":"Kohl","year":"2016","journal-title":"Environ Microbiol"},{"key":"2026092808255251000_ref64","doi-asserted-by":"publisher","first-page":"4099","DOI":"10.1111\/mec.14186","article-title":"Gut microbiota of the pine weevil degrades conifer diterpenes and increases insect fitness","volume":"26","author":"Berasategui","year":"2017","journal-title":"Mol Ecol"},{"key":"2026092808255251000_ref65","doi-asserted-by":"publisher","first-page":"1256","DOI":"10.1128\/AEM.03700-15","article-title":"Deterministic assembly of complex bacterial communities in guts of germ-free cockroaches","volume":"82","author":"Mikaelyan","year":"2016","journal-title":"Appl Environ Microbiol"},{"key":"2026092808255251000_ref66","doi-asserted-by":"publisher","first-page":"639","DOI":"10.1111\/mec.15344","article-title":"The microbiota influences the Drosophila melanogaster life history strategy","volume":"29","author":"Walters","year":"2020","journal-title":"Mol Ecol"},{"key":"2026092808255251000_ref67","doi-asserted-by":"publisher","first-page":"e0011234","DOI":"10.1371\/journal.pntd.0011234","article-title":"A cryopreservation method to recover laboratory- and field-derived bacterial communities from mosquito larval habitats","volume":"17","author":"Zhao","year":"2023","journal-title":"PLoS Negl Trop Dis"},{"key":"2026092808255251000_ref68","doi-asserted-by":"publisher","first-page":"e00959","DOI":"10.1128\/aem.00959-23","article-title":"Introducing an environmental microbiome to axenic Aedes aegypti mosquitoes documents bacterial responses to a blood meal","volume":"89","author":"LaReau","year":"2023","journal-title":"Appl Environ Microbiol"},{"key":"2026092808255251000_ref69","doi-asserted-by":"publisher","first-page":"58","DOI":"10.1186\/s40168-022-01256-5","article-title":"Interspecies microbiome transplantation recapitulates microbial acquisition in mosquitoes","volume":"10","author":"Coon","year":"2022","journal-title":"Microbiome."},{"key":"2026092808255251000_ref70","doi-asserted-by":"publisher","first-page":"207","DOI":"10.1186\/s13071-018-2780-1","article-title":"Diverse laboratory colonies of Aedes aegypti harbor the same adult midgut bacterial microbiome","volume":"11","author":"Dickson","year":"2018","journal-title":"Parasit Vectors"},{"key":"2026092808255251000_ref71","doi-asserted-by":"publisher","first-page":"e0011306","DOI":"10.1371\/journal.pntd.0011306","article-title":"Variable microbiomes between mosquito lines are maintained across different environments","volume":"17","author":"Accoti","year":"2023","journal-title":"PLoS Negl Trop Dis"},{"key":"2026092808255251000_ref72","doi-asserted-by":"publisher","first-page":"e70027","DOI":"10.1111\/1462-2920.70027","article-title":"Mosquitoes reared in nearby insectaries at the same institution have significantly divergent microbiomes","volume":"27","author":"Brettell","year":"2025","journal-title":"Environ Microbiol"},{"key":"2026092808255251000_ref73","doi-asserted-by":"publisher","first-page":"371","DOI":"10.1080\/00034983.1960.11685999","article-title":"A\u00ebdes aegypti as an experimental vector of Brugia Malayi","volume":"54","author":"Ramachandran","year":"1960","journal-title":"Ann Trop Med Parasitol"},{"key":"2026092808255251000_ref74","doi-asserted-by":"publisher","first-page":"957","DOI":"10.1603\/ME10152","article-title":"Early history of laboratory breeding of Aedes aegypti (Diptera: Culicidae) focusing on the origins and use of selected strains","volume":"47","author":"Kuno","year":"2010","journal-title":"J Med Entomol"},{"key":"2026092808255251000_ref75","doi-asserted-by":"publisher","first-page":"5112","DOI":"10.1128\/AEM.01043-13","article-title":"Development of a dual-index sequencing strategy and curation pipeline for analyzing amplicon sequence data on the MiSeq Illumina sequencing platform","volume":"79","author":"Kozich","year":"2013","journal-title":"Appl Environ Microbiol"},{"key":"2026092808255251000_ref76","doi-asserted-by":"publisher","first-page":"581","DOI":"10.1038\/nmeth.3869","article-title":"DADA2: high-resolution sample inference from Illumina amplicon data","volume":"13","author":"Callahan","year":"2016","journal-title":"Nat Methods"},{"key":"2026092808255251000_ref77","doi-asserted-by":"publisher","first-page":"852","DOI":"10.1038\/s41587-019-0209-9","article-title":"Reproducible, interactive, scalable and extensible microbiome data science using QIIME 2","volume":"37","author":"Bolyen","year":"2019","journal-title":"Nat Biotechnol"},{"key":"2026092808255251000_ref78","doi-asserted-by":"publisher","first-page":"90","DOI":"10.1186\/s40168-018-0470-z","article-title":"Optimizing taxonomic classification of marker-gene amplicon sequences with QIIME 2\u2019s q2-feature-classifier plugin","volume":"6","author":"Bokulich","year":"2018","journal-title":"Microbiome."},{"key":"2026092808255251000_ref79","doi-asserted-by":"publisher","first-page":"715","DOI":"10.1038\/s41587-023-01845-1","article-title":"Greengenes2 unifies microbial data in a single reference tree","volume":"42","author":"McDonald","year":"2024","journal-title":"Nat Biotechnol"},{"key":"2026092808255251000_ref80","doi-asserted-by":"publisher","first-page":"772","DOI":"10.1093\/molbev\/mst010","article-title":"MAFFT multiple sequence alignment software version 7: improvements in performance and usability","volume":"30","author":"Katoh","year":"2013","journal-title":"Mol Biol Evol"},{"key":"2026092808255251000_ref81","doi-asserted-by":"publisher","first-page":"e9490","DOI":"10.1371\/journal.pone.0009490","article-title":"FastTree 2 \u2013 approximately maximum-likelihood trees for large alignments","volume":"5","author":"Price","year":"2010","journal-title":"PLoS One"},{"key":"2026092808255251000_ref82","doi-asserted-by":"publisher","DOI":"10.21105\/joss.01299","article-title":"MicrobiomeR: an R package for simplified and standardized microbiome analysis workflows","author":"Gilmore","year":"2019","journal-title":"J Open Source Softw"},{"key":"2026092808255251000_ref83","doi-asserted-by":"publisher","first-page":"e61217","DOI":"10.1371\/journal.pone.0061217","article-title":"Phyloseq: an R package for reproducible interactive analysis and graphics of microbiome census data","volume":"8","author":"McMurdie","year":"2013","journal-title":"PLoS One"},{"key":"2026092808255251000_ref84","doi-asserted-by":"publisher","first-page":"226","DOI":"10.1186\/s40168-018-0605-2","article-title":"Simple statistical identification and removal of contaminant sequences in marker-gene and metagenomics data","volume":"6","author":"Davis","year":"2018","journal-title":"Microbiome."},{"key":"2026092808255251000_ref85","doi-asserted-by":"publisher","first-page":"e00050","DOI":"10.1128\/msystems.00050-22","article-title":"Compositionally aware phylogenetic beta-diversity measures better resolve microbiomes associated with phenotype","volume":"7","author":"Martino","year":"2022","journal-title":"mSystems."},{"key":"2026092808255251000_ref86","doi-asserted-by":"publisher","first-page":"15","DOI":"10.1186\/2049-2618-2-15","article-title":"Unifying the analysis of high-throughput sequencing datasets: characterizing RNA-seq, 16S rRNA gene sequencing and selective growth experiments by compositional data analysis","volume":"2","author":"Fernandes","year":"2014","journal-title":"Microbiome."},{"key":"2026092808255251000_ref87","doi-asserted-by":"publisher","first-page":"2345","DOI":"10.1242\/jeb.054668","article-title":"Variation in metabolic rate of Anopheles gambiae and A. arabiensis in a Sahelian village","volume":"214","author":"Huestis","year":"2011","journal-title":"J Exp Biol"},{"key":"2026092808255251000_ref88","first-page":"86","article-title":"Does temperature affect the outcome of larval competition between Aedes aegypti and Aedes albopictus?","volume":"27","author":"Lounibos","year":"2002","journal-title":"J Vector Ecol"},{"key":"2026092808255251000_ref89","doi-asserted-by":"publisher","first-page":"699","DOI":"10.1603\/0022-2585-39.4.699","article-title":"Pupal mass and wing length as indicators of fecundity in Aedes albopictus and Aedes geniculatus (Diptera: Culicidae)","volume":"39","author":"Armbruster","year":"2002","journal-title":"J Med Entomol"},{"key":"2026092808255251000_ref90","doi-asserted-by":"publisher","first-page":"973","DOI":"10.1111\/jam.14167","article-title":"Faecal freezing preservation period influences colonization ability for faecal microbiota transplantation","volume":"126","author":"Takahashi","year":"2019","journal-title":"J Appl Microbiol"},{"key":"2026092808255251000_ref91","doi-asserted-by":"publisher","first-page":"93","DOI":"10.1038\/s41396-020-00763-3","article-title":"Microbial interactions in the mosquito gut determine Serratia colonization and blood-feeding propensity","volume":"15","author":"Kozlova","year":"2021","journal-title":"ISME J"},{"key":"2026092808255251000_ref92","doi-asserted-by":"publisher","first-page":"e0005677","DOI":"10.1371\/journal.pntd.0005677","article-title":"Amino acid metabolic signaling influences Aedes aegypti midgut microbiome variability","volume":"11","author":"Short","year":"2017","journal-title":"PLoS Negl Trop Dis"},{"key":"2026092808255251000_ref93","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2018.02160","article-title":"Microbiome interaction networks and community structure from laboratory-reared and field-collected Aedes aegypti, Aedes albopictus, and Culex quinquefasciatus mosquito vectors","author":"Hegde","year":"2018","journal-title":"Front Microbiol"},{"key":"2026092808255251000_ref94","doi-asserted-by":"publisher","first-page":"5619","DOI":"10.3390\/v7102886","article-title":"Exposure to West Nile virus increases bacterial diversity and immune gene expression in Culex pipiens","volume":"7","author":"Zink","year":"2015","journal-title":"Viruses."},{"key":"2026092808255251000_ref95","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.03733-14","article-title":"Elizabethkingia anophelis: molecular manipulation and interactions with mosquito hosts","author":"Chen","journal-title":"Appl Environ Microbiol"},{"key":"2026092808255251000_ref96","doi-asserted-by":"publisher","DOI":"10.21203\/rs.3.rs-8191790\/v1","article-title":"Microsporidia MB mediated transcriptomics and gut microbiota shift in Plasmodium falciparum exposed Anopheles arabiensis mosquitoes","author":"Waweru","year":"2025"},{"key":"2026092808255251000_ref97","doi-asserted-by":"publisher","first-page":"e2424570122","DOI":"10.1073\/pnas.2424570122","article-title":"Host complement C3 promotes malaria transmission by killing symbiotic bacteria in the mosquito midgut","volume":"122","author":"He","year":"2025","journal-title":"Proc Natl Acad Sci"},{"key":"2026092808255251000_ref98","doi-asserted-by":"publisher","first-page":"45","DOI":"10.1186\/s12936-024-04870-w","article-title":"Two promising candidates for paratransgenesis, Elizabethkingia and Asaia, increase in both sexes of Anopheles gambiae mosquitoes after feeding","volume":"23","author":"Egyirifa","year":"2024","journal-title":"Malar J"},{"key":"2026092808255251000_ref99","doi-asserted-by":"publisher","first-page":"1279","DOI":"10.1002\/cbic.201900675","article-title":"Coculturing of mosquito-microbiome bacteria promotes heme degradation in Elizabethkingia anophelis","volume":"21","author":"Ganley","year":"2020","journal-title":"ChemBioChem."},{"key":"2026092808255251000_ref100","doi-asserted-by":"publisher","first-page":"4365","DOI":"10.1038\/s41598-021-83638-7","article-title":"High engraftment capacity of frozen ready-to-use human fecal microbiota transplants assessed in germ-free mice","volume":"11","author":"Berland","year":"2021","journal-title":"Sci Rep"},{"key":"2026092808255251000_ref101","doi-asserted-by":"publisher","DOI":"10.3389\/fcimb.2020.00077","article-title":"Changes in microbiota profiles after prolonged frozen storage of stool suspensions","volume":"10","author":"Dorsaz","year":"2020","journal-title":"Front Cell Infect Microbiol"},{"key":"2026092808255251000_ref102","doi-asserted-by":"publisher","first-page":"4856","DOI":"10.3390\/app14114856","article-title":"Evaluation of the suitability of selecting a faecal microbiota transplant: bacterial composition and subsequent long-term monitoring of the viability of its frozen and lyophilised forms","volume":"14","author":"Pacuta","year":"2024","journal-title":"Appl Sci"},{"key":"2026092808255251000_ref103","doi-asserted-by":"publisher","first-page":"8897","DOI":"10.1038\/s41598-019-45173-4","article-title":"A guide for ex vivo handling and storage of stool samples intended for fecal microbiota transplantation","volume":"9","author":"Burz","year":"2019","journal-title":"Sci Rep"},{"key":"2026092808255251000_ref104","doi-asserted-by":"publisher","first-page":"1011","DOI":"10.1111\/apt.13366","article-title":"Faecal microbiota transplant for recurrent Clostridium difficile infection using long-term frozen stool is effective: clinical efficacy and bacterial viability data","volume":"42","author":"Costello","year":"2015","journal-title":"Aliment Pharmacol Ther"},{"key":"2026092808255251000_ref105","doi-asserted-by":"publisher","first-page":"142","DOI":"10.1001\/jama.2015.18098","article-title":"Frozen vs fresh Fecal microbiota transplantation and clinical resolution of diarrhea in patients with recurrent Clostridium difficile infection: a randomized clinical trial","volume":"315","author":"Lee","year":"2016","journal-title":"JAMA."},{"key":"2026092808255251000_ref106","doi-asserted-by":"publisher","first-page":"1985","DOI":"10.1001\/jama.2017.17077","article-title":"Effect of oral capsule\u2013 vs colonoscopy-delivered fecal microbiota transplantation on recurrent Clostridium difficile infection: a randomized clinical trial","volume":"318","author":"Kao","year":"2017","journal-title":"JAMA."},{"key":"2026092808255251000_ref107","doi-asserted-by":"publisher","first-page":"239","DOI":"10.1097\/MCG.0000000000001777","article-title":"Fresh versus frozen versus lyophilized fecal microbiota transplant for recurrent Clostridium difficile infection: a systematic review and network meta-analysis","volume":"57","author":"Gangwani","year":"2023","journal-title":"J Clin Gastroenterol"},{"key":"2026092808255251000_ref108","doi-asserted-by":"publisher","first-page":"7144","DOI":"10.1038\/s41598-020-64163-5","article-title":"Role of plants in the transmission of Asaia sp., which potentially inhibit the Plasmodium sporogenic cycle in Anopheles mosquitoes","volume":"10","author":"Bassene","year":"2020","journal-title":"Sci Rep"},{"key":"2026092808255251000_ref109","doi-asserted-by":"publisher","first-page":"305","DOI":"10.1179\/2047773213Y.0000000106","article-title":"Asaia accelerates larval development of Anopheles gambiae","volume":"107","author":"Mitraka","year":"2013","journal-title":"Pathog Glob Health"},{"key":"2026092808255251000_ref110","doi-asserted-by":"publisher","first-page":"lxae261","DOI":"10.1093\/jambio\/lxae261","article-title":"aaccelerate development of the yellow fever mosquito, Aedes aegypti, via interactions with the vertically transmitted larval microbiome","author":"Roman","year":"2024","journal-title":"J Appl Microbiol"},{"key":"2026092808255251000_ref111","doi-asserted-by":"publisher","first-page":"97","DOI":"10.1186\/s12915-023-01605-2","article-title":"Egg-laying by female Aedes aegypti shapes the bacterial communities of breeding sites","volume":"21","author":"Mosquera","year":"2023","journal-title":"BMC Biol"},{"key":"2026092808255251000_ref112","doi-asserted-by":"publisher","DOI":"10.1186\/s40168-026-02477-8","article-title":"Vertical transmission of mosquito microbiota and its effects on offspring development","author":"Nichols","year":"2025"},{"key":"2026092808255251000_ref113","doi-asserted-by":"publisher","first-page":"387","DOI":"10.1186\/s13104-025-07482-y","article-title":"Indicator bacteria taxa reveal breeding site origins of Aedes aegypti mosquitoes","volume":"18","author":"Mosquera","year":"2025","journal-title":"BMC Res Notes"},{"key":"2026092808255251000_ref114","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2021.624170","article-title":"Exploring changes in the microbiota of Aedes albopictus: comparison among breeding site water, larvae, and adults","volume":"12","author":"Scolari","year":"2021","journal-title":"Front Microbiol"},{"key":"2026092808255251000_ref115","doi-asserted-by":"publisher","first-page":"74","DOI":"10.4103\/0972-9062.289392","article-title":"Characterization of midgut microbiome of Anopheles stephensi Liston","volume":"58","author":"Pereira","year":"2021","journal-title":"J Vector Borne Dis"},{"key":"2026092808255251000_ref116","doi-asserted-by":"publisher","first-page":"414","DOI":"10.1186\/s12936-021-03934-5","article-title":"Comprehensive characterization of internal and cuticle surface microbiota of laboratory-reared F1 Anopheles albimanus originating from different sites","volume":"20","author":"Dada","year":"2021","journal-title":"Malar J"},{"key":"2026092808255251000_ref117","doi-asserted-by":"publisher","first-page":"63","DOI":"10.1093\/jme\/tju011","article-title":"Bacterial communities and midgut microbiota associated with mosquito populations from waste tires in East-Central Illinois","volume":"52","author":"Kim","year":"2015","journal-title":"J Med Entomol"},{"key":"2026092808255251000_ref118","doi-asserted-by":"publisher","DOI":"10.3389\/fcimb.2023.1162918","article-title":"A rare sugar, allose, inhibits the development of Plasmodium parasites in the Anopheles mosquito independently of midgut microbiota","author":"Mizushima","year":"2023","journal-title":"Front Cell Infect Microbiol"},{"key":"2026092808255251000_ref119","doi-asserted-by":"publisher","first-page":"330","DOI":"10.1186\/s13071-024-06421-3","article-title":"Relationship between deltamethrin resistance and gut symbiotic bacteria of Aedes albopictus by 16S rDNA sequencing","volume":"17","author":"Sun","year":"2024","journal-title":"Parasites Vectors"},{"key":"2026092808255251000_ref120","doi-asserted-by":"publisher","first-page":"4464","DOI":"10.1038\/s41467-018-07014-2","article-title":"Generation of axenic Aedes aegypti demonstrate live bacteria are not required for mosquito development","volume":"9","author":"Correa","year":"2018","journal-title":"Nat Commun"}],"container-title":["ISME Host Microbe"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/academic.oup.com\/ismehm\/advance-article-pdf\/doi\/10.1093\/ismehm\/aazag010\/71226410\/aazag010.pdf","content-type":"application\/pdf","content-version":"am","intended-application":"syndication"},{"URL":"https:\/\/academic.oup.com\/ismehm\/article-pdf\/1\/1\/aazag010\/71226410\/aazag010.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/academic.oup.com\/ismehm\/article-pdf\/1\/1\/aazag010\/71226410\/aazag010.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,9,28]],"date-time":"2026-09-28T12:25:58Z","timestamp":1790598358000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/ismehm\/article\/doi\/10.1093\/ismehm\/aazag010\/8817329"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026]]},"references-count":120,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2026,8,5]]}},"URL":"https:\/\/doi.org\/10.1093\/ismehm\/aazag010","relation":{},"ISSN":["2978-8285"],"issn-type":[{"value":"2978-8285","type":"electronic"}],"subject":[],"published-other":{"date-parts":[[2026]]},"published":{"date-parts":[[2026]]},"article-number":"aazag010"}}