{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,17]],"date-time":"2026-08-17T23:23:39Z","timestamp":1787009019663,"version":"build-2736575974"},"reference-count":92,"publisher":"Oxford University Press (OUP)","issue":"1","license":[{"start":{"date-parts":[[2024,5,31]],"date-time":"2024-05-31T00:00:00Z","timestamp":1717113600000},"content-version":"vor","delay-in-days":151,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"University Consortium for Field-Focused Groundwater Research and Corteva Agriscience"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2024,1,8]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Isolate studies have been a cornerstone for unraveling metabolic pathways and phenotypical (functional) features. Biogeochemical processes in natural and engineered ecosystems are generally performed by more than a single microbe and often rely on mutualistic interactions. We demonstrate the rational bottom-up design of synthetic, interdependent co-cultures to achieve concomitant utilization of chlorinated methanes as electron donors and organohalogens as electron acceptors. Specialized anaerobes conserve energy from the catabolic conversion of chloromethane or dichloromethane to formate, H2, and acetate, compounds that the organohalide-respiring bacterium Dehalogenimonas etheniformans strain GP requires to utilize cis-1,2-dichloroethenene and vinyl chloride as electron acceptors. Organism-specific qPCR enumeration matched the growth of individual dechlorinators to the respective functional (i.e. dechlorination) traits. The metabolite cross-feeding in the synthetic (co-)cultures enables concomitant utilization of chlorinated methanes (i.e. chloromethane and dichloromethane) and chlorinated ethenes (i.e. cis-1,2-dichloroethenene and vinyl chloride) without the addition of an external electron donor (i.e. formate and H2). The findings illustrate that naturally occurring chlorinated C1 compounds can sustain anaerobic food webs, an observation with implications for the development of interdependent, mutualistic communities, the sustenance of microbial life in oligotrophic and energy-deprived environments, and the fate of chloromethane\/dichloromethane and chlorinated electron acceptors (e.g. chlorinated ethenes) in pristine environments and commingled contaminant plumes.<\/jats:p>","DOI":"10.1093\/ismejo\/wrae090","type":"journal-article","created":{"date-parts":[[2024,5,31]],"date-time":"2024-05-31T14:17:03Z","timestamp":1717165023000},"source":"Crossref","is-referenced-by-count":15,"title":["Metabolite cross-feeding enables concomitant catabolism of chlorinated methanes and chlorinated ethenes in synthetic microbial assemblies"],"prefix":"10.1093","volume":"18","author":[{"given":"Gao","family":"Chen","sequence":"first","affiliation":[{"name":"Department of Civil and Environmental Engineering, University of Tennessee , Knoxville, TN 37996 , United States"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yi","family":"Yang","sequence":"additional","affiliation":[{"name":"Key Laboratory of Pollution Ecology and Environmental Engineering, Institute of Applied Ecology, Chinese Academy of Sciences , Shenyang, Liaoning 110016 , China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jun","family":"Yan","sequence":"additional","affiliation":[{"name":"Key Laboratory of Pollution Ecology and Environmental Engineering, Institute of Applied Ecology, Chinese Academy of Sciences , Shenyang, Liaoning 110016 , China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Frank E","family":"L\u00f6ffler","sequence":"additional","affiliation":[{"name":"Department of Civil and Environmental Engineering, University of Tennessee , Knoxville, TN 37996 , United States"},{"name":"Department of Microbiology, University of Tennessee , Knoxville, TN 37996 , United States"},{"name":"Department of Biosystems Engineering and Soil Science, University of Tennessee , Knoxville, TN 37996 , United States"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2024,5,31]]},"reference":[{"key":"2024061312460436200_ref1","doi-asserted-by":"crossref","first-page":"1224","DOI":"10.1038\/s41559-021-01505-0","article-title":"Obligate cross-feeding expands the metabolic niche of bacteria","volume":"5","author":"O\u00f1a","year":"2021","journal-title":"Nat Ecol Evol"},{"key":"2024061312460436200_ref2","doi-asserted-by":"crossref","first-page":"e00135","DOI":"10.1128\/MMBR.00135-20","article-title":"Extracellular metabolism sets the table for microbial cross-feeding","volume":"85","author":"Fritts","year":"2021","journal-title":"Microbiol Mol Biol Rev"},{"key":"2024061312460436200_ref3","doi-asserted-by":"crossref","first-page":"23","DOI":"10.1038\/s43705-021-00025-z","article-title":"Substrate-dependent 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