{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,2]],"date-time":"2026-07-02T14:10:51Z","timestamp":1783001451843,"version":"3.54.5"},"reference-count":70,"publisher":"Oxford University Press (OUP)","issue":"1","license":[{"start":{"date-parts":[[2024,6,19]],"date-time":"2024-06-19T00:00:00Z","timestamp":1718755200000},"content-version":"vor","delay-in-days":170,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["32270680"],"award-info":[{"award-number":["32270680"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Shenzhen-Hong Kong Institute of Brain Science-Shenzhen Fundamental Research Institutions","award":["2023SHIBS0003"],"award-info":[{"award-number":["2023SHIBS0003"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2024,1,8]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>Selenocysteine (Sec) is encoded by the UGA codon that normally functions as a stop signal and is specifically incorporated into selenoproteins via a unique recoding mechanism. The translational recoding of UGA as Sec is directed by an unusual RNA structure, the SECIS element. Although archaea and eukaryotes adopt similar Sec encoding machinery, the SECIS elements have no similarities to each other with regard to sequence and structure. We analyzed &amp;gt;400 Asgard archaeal genomes to examine the occurrence of both Sec encoding system and selenoproteins in this archaeal superphylum, the closest prokaryotic relatives of eukaryotes. A comprehensive map of Sec utilization trait has been generated, providing the most detailed understanding of the use of this nonstandard amino acid in Asgard archaea so far. By characterizing the selenoproteomes of all organisms, several selenoprotein-rich phyla and species were identified. Most Asgard archaeal selenoprotein genes possess eukaryotic SECIS-like structures with varying degrees of diversity. Moreover, euryarchaeal SECIS elements might originate from Asgard archaeal SECIS elements via lateral gene transfer, indicating a complex and dynamic scenario of the evolution of SECIS element within archaea. Finally, a roadmap for the transition of eukaryotic SECIS elements from archaea was proposed, and selenophosphate synthetase may serve as a potential intermediate for the generation of ancestral eukaryotic SECIS element. Our results offer new insights into a deeper understanding of the evolution of Sec insertion machinery.<\/jats:p>","DOI":"10.1093\/ismejo\/wrae111","type":"journal-article","created":{"date-parts":[[2024,6,18]],"date-time":"2024-06-18T06:38:57Z","timestamp":1718692737000},"source":"Crossref","is-referenced-by-count":5,"title":["Asgard archaeal selenoproteome reveals a roadmap for the archaea-to-eukaryote transition of selenocysteine incorporation machinery"],"prefix":"10.1093","volume":"18","author":[{"given":"Biyan","family":"Huang","sequence":"first","affiliation":[{"name":"Shenzhen Key Laboratory of Marine Bioresources and Ecology , Brain Disease and Big Data Research Institute, College of Life Sciences and Oceanography, , Shenzhen 518055, Guangdong Province,","place":["P. R. China"]},{"name":"Shenzhen University , Brain Disease and Big Data Research Institute, College of Life Sciences and Oceanography, , Shenzhen 518055, Guangdong Province,","place":["P. R. China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yao","family":"Xiao","sequence":"additional","affiliation":[{"name":"Shenzhen Key Laboratory of Marine Bioresources and Ecology , Brain Disease and Big Data Research Institute, College of Life Sciences and Oceanography, , Shenzhen 518055, Guangdong Province,","place":["P. R. China"]},{"name":"Shenzhen University , Brain Disease and Big Data Research Institute, College of Life Sciences and Oceanography, , Shenzhen 518055, Guangdong Province,","place":["P. R. China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yan","family":"Zhang","sequence":"additional","affiliation":[{"name":"Shenzhen Key Laboratory of Marine Bioresources and Ecology , Brain Disease and Big Data Research Institute, College of Life Sciences and Oceanography, , Shenzhen 518055, Guangdong Province,","place":["P. R. China"]},{"name":"Shenzhen University , Brain Disease and Big Data Research Institute, College of Life Sciences and Oceanography, , Shenzhen 518055, Guangdong Province,","place":["P. R. China"]},{"name":"Shenzhen-Hong Kong Institute of Brain Science-Shenzhen Fundamental Research Institutions , Shenzhen 518055, Guangdong Province,","place":["P. R. China"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2024,6,19]]},"reference":[{"key":"2025022210114426700_ref1","doi-asserted-by":"crossref","first-page":"25","DOI":"10.1039\/C3MT00185G","article-title":"Selenium biochemistry and its role for human health","volume":"6","author":"Roman","year":"2014","journal-title":"Metallomics"},{"key":"2025022210114426700_ref2","doi-asserted-by":"crossref","first-page":"598","DOI":"10.2174\/1389203715666140608151134","article-title":"Selenium and selenoproteins: an overview on different biological systems","volume":"15","author":"Mangiapane","year":"2014","journal-title":"Curr Protein Pept Sci"},{"key":"2025022210114426700_ref3","doi-asserted-by":"crossref","first-page":"1561","DOI":"10.1016\/j.biochi.2006.04.015","article-title":"Selenoprotein synthesis: UGA does not end the 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