{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,17]],"date-time":"2026-08-17T23:23:41Z","timestamp":1787009021626,"version":"build-2736575974"},"reference-count":64,"publisher":"Oxford University Press (OUP)","issue":"1","license":[{"start":{"date-parts":[[2024,7,20]],"date-time":"2024-07-20T00:00:00Z","timestamp":1721433600000},"content-version":"vor","delay-in-days":201,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Science Foundation of Fujian Province"},{"name":"Outstanding Young Research Talents Program of Fujian Agriculture and Forestry University"},{"DOI":"10.13039\/501100001809","name":"National Science Foundation of China","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100012166","name":"National Key R&D Program of China","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2024,1,8]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Arthropods, such as houseflies, play a significant role in the dissemination of antimicrobial resistance (AMR); however, their impact has often been overlooked in comparison to other AMR vectors. Understanding the contribution of arthropods to the spread of AMR is critical for implementing robust policies to mitigate the spread of AMR across One Health sectors, affecting animals and environmental habitats as well as humans. In this study, we investigated the in situ transfer of a gfp-labelled AMR plasmid (IncA\/C carrying an mcr-8 gene, pA\/C_MCR-8) in the gut microbiota of houseflies (Musca domestica) by applying single-cell sorting, 16S rRNA gene amplicon sequencing and whole-genome sequencing. Our findings demonstrate that the pA\/C_MCR-8\u2013positive Escherichia coli donor strain is capable of colonizing the gut microbiome of houseflies and persists in the housefly intestine for 5 days; however, no transfer was detectable above the detection threshold of 10\u22125 per cell. The conjugative plasmid pA\/C_MCR-8 demonstrated a high transfer frequency ranging from 4.1\u2009\u00d7\u200910\u22123 to 5.0\u2009\u00d7\u200910\u22123 per cell in vitro and exhibited transfer across various bacterial phyla, primarily encompassing Pseudomonadota and Bacillota. Phylogenic analysis has revealed that Providencia stuartii, a human opportunistic pathogen, is a notable recipient of pA\/C_MCR-8. The conjugation assays further revealed that newly formed P. stuartii transconjugants readily transfer pA\/C_MCR-8 to other clinically relevant pathogens (e.g. Klebsiella pneumoniae). Our findings indicate the potential transfer of AMR plasmids from houseflies to human opportunistic pathogens and further support the adoption of a One Health approach in developing infection control policies that address AMR across clinical settings.<\/jats:p>","DOI":"10.1093\/ismejo\/wrae128","type":"journal-article","created":{"date-parts":[[2024,7,18]],"date-time":"2024-07-18T09:17:16Z","timestamp":1721294236000},"source":"Crossref","is-referenced-by-count":18,"title":["Housefly gut microbiomes as a reservoir and facilitator for the spread of antibiotic resistance"],"prefix":"10.1093","volume":"18","author":[{"given":"Dehao","family":"Gan","sequence":"first","affiliation":[{"name":"Fujian Provincial Key Laboratory of Soil Environmental Health and Regulation , College of Resources and Environment, , Fuzhou 350002 ,","place":["China"]},{"name":"Fujian Agriculture and Forestry University , College of Resources and Environment, , Fuzhou 350002 ,","place":["China"]},{"name":"Fujian Key Laboratory of Traditional Chinese Veterinary Medicine and Animal Health , College of Animal Sciences, , Fuzhou 350002 ,","place":["China"]},{"name":"Fujian Agriculture and Forestry University , College of Animal Sciences, , Fuzhou 350002 ,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhenyan","family":"Lin","sequence":"additional","affiliation":[{"name":"Fujian Provincial Key Laboratory of Soil Environmental Health and Regulation , College of Resources and Environment, , Fuzhou 350002 ,","place":["China"]},{"name":"Fujian Agriculture and Forestry University , College of Resources and Environment, , Fuzhou 350002 ,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lingshuang","family":"Zeng","sequence":"additional","affiliation":[{"name":"Fujian Provincial Key Laboratory of Soil Environmental Health and Regulation , College of Resources and Environment, , Fuzhou 350002 ,","place":["China"]},{"name":"Fujian Agriculture and Forestry University , College of Resources and Environment, , Fuzhou 350002 ,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hui","family":"Deng","sequence":"additional","affiliation":[{"name":"Fujian Key Laboratory of Traditional Chinese Veterinary Medicine and Animal Health , College of Animal Sciences, , Fuzhou 350002 ,","place":["China"]},{"name":"Fujian Agriculture and Forestry University , College of Animal Sciences, , Fuzhou 350002 ,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Timothy R","family":"Walsh","sequence":"additional","affiliation":[{"name":"Ineos Oxford Institute for Antimicrobial Research , Department of Biology, , Oxford OX1 3RE ,","place":["United Kingdom"]},{"name":"University of Oxford , Department of Biology, , Oxford OX1 3RE ,","place":["United 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