{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,24]],"date-time":"2026-09-24T14:59:27Z","timestamp":1790261967439,"version":"4.1.0"},"reference-count":24,"publisher":"Oxford University Press (OUP)","issue":"1","license":[{"start":{"date-parts":[[2024,12,10]],"date-time":"2024-12-10T00:00:00Z","timestamp":1733788800000},"content-version":"vor","delay-in-days":344,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"German Ministry of Education and Research, BMBF","award":["01KI1909A (EMBARK)"],"award-info":[{"award-number":["01KI1909A (EMBARK)"]}]},{"name":"German Ministry of Education and Research, BMBF","award":["01KI2401 (TEXAS)"],"award-info":[{"award-number":["01KI2401 (TEXAS)"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2024,1,8]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>It is consensus that clinically relevant antibiotic resistance genes have their origin in environmental bacteria, including the large pool of primarily benign species. Yet, for the vast majority of acquired antibiotic resistance genes, the original environmental host(s) has not been identified to date. Closing this knowledge gap could improve our understanding of how antimicrobial resistance proliferates in the bacterial domain and shed light on the crucial step of initial resistance gene mobilization in particular. Here, we combine information from publicly available long- and short-read environmental metagenomes as well as whole-genome sequences to identify the original environmental hosts of dfrB, a family of genes conferring resistance to trimethoprim. Although this gene family stands in the shadow of the more widespread, structurally different dfrA, it has recently gained attention through the discovery of several new members. Based on the genetic context of dfrB observed in long-read metagenomes, we predicted bacteria of the order Burkholderiales to function as original environmental hosts of the predominant gene variants in both soil and freshwater. The predictions were independently confirmed by whole-genome datasets and statistical correlations between dfrB abundance and taxonomic composition of environmental bacterial communities. Our study suggests that Burkholderiales in general and the family Comamonadaceae in particular represent environmental origins of dfrB genes, some of which now contribute to the acquired resistome of facultative pathogens. We propose that our workflow centered on long-read environmental metagenomes allows for the identification of the original hosts of further clinically relevant antibiotic resistance genes.<\/jats:p>","DOI":"10.1093\/ismejo\/wrae243","type":"journal-article","created":{"date-parts":[[2024,12,6]],"date-time":"2024-12-06T07:31:54Z","timestamp":1733470314000},"source":"Crossref","is-referenced-by-count":11,"title":["Bacteria of the order\n                    <i>Burkholderiales<\/i>\n                    are original environmental hosts of type II trimethoprim resistance genes (\n                    <i>dfrB<\/i>\n                    )"],"prefix":"10.1093","volume":"18","author":[{"given":"David","family":"Kneis","sequence":"first","affiliation":[{"name":"Dresden University of Technology, Institute of Hydrobiology , 01062 Dresden, Saxony ,","place":["Germany"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Faina","family":"Tskhay","sequence":"additional","affiliation":[{"name":"Dresden University of Technology, Institute of Hydrobiology , 01062 Dresden, Saxony ,","place":["Germany"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Magali","family":"de la Cruz Barron","sequence":"additional","affiliation":[{"name":"Dresden University of Technology, Institute of Hydrobiology , 01062 Dresden, Saxony ,","place":["Germany"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thomas U","family":"Berendonk","sequence":"additional","affiliation":[{"name":"Dresden University of Technology, Institute of Hydrobiology , 01062 Dresden, Saxony 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