{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,25]],"date-time":"2026-04-25T01:46:38Z","timestamp":1777081598533,"version":"3.51.4"},"reference-count":109,"publisher":"Oxford University Press (OUP)","issue":"1","license":[{"start":{"date-parts":[[2025,7,21]],"date-time":"2025-07-21T00:00:00Z","timestamp":1753056000000},"content-version":"vor","delay-in-days":201,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000001","name":"National Science Foundation","doi-asserted-by":"publisher","award":["EF-2125065, EF-2125066, EF-2125067, and CAREER-2236580 to D. M"],"award-info":[{"award-number":["EF-2125065, EF-2125066, EF-2125067, and CAREER-2236580 to D. 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Absolute abundance identified key genera, Lactococcus, Parabacteroides, and Cetobacterium in salamanders, and Basidiobolus and Mortierella in lizards, turtles, snakes, and tortoises, that consistently emerged as core taxa, revealing host-associated patterns previously obscured by compositional constraints. In closely related Desmognathus species, where environmental and phylogenetic variation was minimized, absolute abundance enabled finer resolution of microbiome dynamics and significantly reduced false discovery rates. Absolute abundance-based network analyses further revealed distinct keystone taxa between the relative and absolute abundance datasets. Despite low redundancy, Basidiobolus exhibited high network betweenness, efficiency, and degree, suggesting its role as a key connector between microbial modules and a contributor to overall network robustness. This predicted structural role aligns with Burt\u2019s structural hole theory, which suggests that nodes linking otherwise disconnected modules occupy influential network positions. These findings underscore the value of absolute abundance in resolving microbial dynamics and supporting meaningful interpretation of host-microbiome associations. This advance is made possible by DspikeIn, a flexible wet-lab and computational framework that enhances ecological resolution and cross-study comparability.<\/jats:p>","DOI":"10.1093\/ismejo\/wraf150","type":"journal-article","created":{"date-parts":[[2025,7,19]],"date-time":"2025-07-19T11:53:45Z","timestamp":1752926025000},"source":"Crossref","is-referenced-by-count":6,"title":["Absolute abundance unveils\n                    <i>Basidiobolus<\/i>\n                    as a cross-domain bridge indirectly bolstering gut microbiome homeostasis"],"prefix":"10.1093","volume":"19","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9185-9993","authenticated-orcid":false,"given":"Mitra","family":"Ghotbi","sequence":"first","affiliation":[{"name":"Department of Biology, Middle Tennessee State University, 1301 East Main Street, Murfreesboro, TN 37132, United 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Germany"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Joseph W","family":"Spatafora","sequence":"additional","affiliation":[{"name":"Department of Botany and Plant Pathology, Oregon State University, 2701 SW Campus Way, Corvallis, OR 97331, United States"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kian","family":"Kelly","sequence":"additional","affiliation":[{"name":"Department of Plant Pathology & Microbiology, University of California Riverside, 900 University Avenue, Riverside, CA 92521, United States"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0004-7271-5520","authenticated-orcid":false,"given":"Nicholas Reed","family":"Alexander","sequence":"additional","affiliation":[{"name":"Department of Biology, Middle Tennessee State University, 1301 East Main Street, Murfreesboro, TN 37132, United States"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kylie C","family":"Moe","sequence":"additional","affiliation":[{"name":"Department of Biology, Middle Tennessee State University, 1301 East Main Street, Murfreesboro, TN 37132, United States"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kimberly C","family":"Syring","sequence":"additional","affiliation":[{"name":"Department of Botany and Plant Pathology, Oregon State University, 2701 SW Campus Way, Corvallis, OR 97331, United States"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Leila","family":"Shadmani","sequence":"additional","affiliation":[{"name":"Department of Plant Pathology & Microbiology, University of California Riverside, 900 University Avenue, Riverside, CA 92521, United States"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Julissa","family":"Perez-Marron","sequence":"additional","affiliation":[{"name":"Department of Plant Pathology & Microbiology, University of California Riverside, 900 University Avenue, Riverside, CA 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