{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,16]],"date-time":"2026-09-16T17:36:00Z","timestamp":1789580160077,"version":"build-2803163510"},"reference-count":79,"publisher":"Oxford University Press (OUP)","issue":"1","license":[{"start":{"date-parts":[[2025,8,28]],"date-time":"2025-08-28T00:00:00Z","timestamp":1756339200000},"content-version":"vor","delay-in-days":239,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000203","name":"U.S. Geological Survey","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100000203","id-type":"DOI","asserted-by":"publisher"}]},{"name":"U.S. Department of Energy (DOE) Office of Science, Office of Biological and Environmental Research","award":["DE-SC0023084"],"award-info":[{"award-number":["DE-SC0023084"]}]},{"DOI":"10.13039\/100000001","name":"National Science Foundation","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100000001","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2025,1,2]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>Wetlands are a major source of methane emissions and contribute to the observed increase in atmospheric methane over the last 20\u00a0years. Methane production in wetlands is the final step of carbon decomposition performed by anaerobic archaea. Although hydrogen\/carbon dioxide and acetate are the substrates most often attributed to methanogenesis, other substrates\u2014such as methylated compounds\u2014may additionally play important roles in driving methane production in wetland systems. Here we conducted mesocosm experiments combined with genome-resolved metatranscriptomics to investigate the impact of diverse methanogenic substrate amendment on methanogenesis in two high methane-emitting wetlands with distinct geochemistry, termed P7 and P8. Methanol amendment resulted in high methane production at both sites, whereas acetate and formate amendment only stimulated methanogenesis in P7 mesocosms, where aqueous sulfide concentrations were lower. In P7 sediments, formate amendment fueled acetogenic microbes that produced acetate, which was subsequently utilized by acetoclastic methanogens. In contrast to expression profiles in P7 mesocosms, active methylotrophic methanogen genomes from P8 showed increased expression of genes related to membrane remodeling and DNA damage repair, indicative of stress tolerance mechanisms to counter sulfide toxicity. Methylotrophic methanogenesis generates higher free energy yields than acetoclastic methanogenesis, which likely enables allocation of more energy toward stress responses. These findings contribute to the growing body of literature highlighting methylotrophic methanogenesis as an important methane production pathway in wetlands. By using less competitive substrates like methanol that provide greater energy yields, methylotrophic methanogens may invest in physiological strategies that provide competitive advantages across a range of environmental stresses.<\/jats:p>","DOI":"10.1093\/ismejo\/wraf196","type":"journal-article","created":{"date-parts":[[2025,8,28]],"date-time":"2025-08-28T11:21:44Z","timestamp":1756380104000},"source":"Crossref","is-referenced-by-count":7,"title":["Sulfide stress tolerance as a controller of methane production in temperate wetlands"],"prefix":"10.1093","volume":"19","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5092-6264","authenticated-orcid":false,"given":"Emily K","family":"Bechtold","sequence":"first","affiliation":[{"name":"Department of Soil and Crop Sciences, Colorado State University , Fort Collins, CO 80523 ,","place":["United States"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1692-9044","authenticated-orcid":false,"given":"Jared B","family":"Ellenbogen","sequence":"additional","affiliation":[{"name":"Department of Soil and Crop Sciences, Colorado State University , Fort Collins, CO 80523 ,","place":["United States"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5267-9727","authenticated-orcid":false,"given":"Danhui","family":"Xin","sequence":"additional","affiliation":[{"name":"Department of Civil, Construction, and Environmental Engineering, University of Delaware , Newark, DE 19716 ,","place":["United States"]},{"name":"Southern California Coastal Water Research Project Authority , Chemistry Department, Costa Mesa, CA 92626 ,","place":["United States"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1010-5095","authenticated-orcid":false,"given":"Maricia","family":"Pacheco","sequence":"additional","affiliation":[{"name":"Department of Soil, Water, and Climate, University of Minnesota , Minneapolis, MN 55455 ,","place":["United States"]},{"name":"Department of Civil, Environmental, and Geo- Engineering, University of Minnesota , Minneapolis, MN 55455 ,","place":["United States"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3681-3455","authenticated-orcid":false,"given":"Brandy M","family":"Toner","sequence":"additional","affiliation":[{"name":"Department of Soil, Water, and Climate, University of Minnesota , Minneapolis, MN 55455 ,","place":["United States"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1427-9156","authenticated-orcid":false,"given":"Yu-Ping","family":"Chin","sequence":"additional","affiliation":[{"name":"Department of Civil, Construction, and Environmental Engineering, University of Delaware , Newark, DE 19716 ,","place":["United States"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0814-5469","authenticated-orcid":false,"given":"William A","family":"Arnold","sequence":"additional","affiliation":[{"name":"Department of Civil, Environmental, and Geo- Engineering, University of Minnesota , Minneapolis, MN 55455 ,","place":["United States"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1233-1707","authenticated-orcid":false,"given":"Sheel","family":"Bansal","sequence":"additional","affiliation":[{"name":"Northern Prairie Wildlife Research Center, U. 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Geological Survey , Jamestown, ND 5840 ,","place":["United States"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3595-0853","authenticated-orcid":false,"given":"Michael J","family":"Wilkins","sequence":"additional","affiliation":[{"name":"Department of Soil and Crop Sciences, Colorado State University , Fort Collins, CO 80523 ,","place":["United States"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2025,8,28]]},"reference":[{"key":"2025091917493036300_ref1","doi-asserted-by":"publisher","first-page":"470","DOI":"10.1038\/s43247-024-01635-w","article-title":"Wetland hydrological dynamics and methane emissions","volume":"5","author":"Cui","year":"2024","journal-title":"Commun Earth Environ"},{"key":"2025091917493036300_ref2","doi-asserted-by":"publisher","first-page":"12614","DOI":"10.1002\/2016GL071930","article-title":"Radiative forcing of carbon dioxide, methane, and nitrous 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