{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T21:20:09Z","timestamp":1791321609442,"version":"4.3.1"},"reference-count":68,"publisher":"Oxford University Press (OUP)","issue":"1","license":[{"start":{"date-parts":[[2026,7,2]],"date-time":"2026-07-02T00:00:00Z","timestamp":1782950400000},"content-version":"vor","delay-in-days":182,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000002","name":"NIH","doi-asserted-by":"publisher","award":["1S10OD010786-01"],"award-info":[{"award-number":["1S10OD010786-01"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"name":"HADES-ERC","award":["669947"],"award-info":[{"award-number":["669947"]}]},{"name":"Genoscope-CEA"},{"name":"France G\u00e9nomique"},{"DOI":"10.13039\/100009111","name":"Ifremer","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100009111","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Chilean Agency for Research and Development","award":["ICN12_019N-IMO"],"award-info":[{"award-number":["ICN12_019N-IMO"]}]},{"DOI":"10.13039\/501100001732","name":"Danish National Research Foundation","doi-asserted-by":"publisher","award":["DNRF145"],"award-info":[{"award-number":["DNRF145"]}],"id":[{"id":"10.13039\/501100001732","id-type":"DOI","asserted-by":"publisher"}]},{"name":"National Science Foundation Ocean Technology and Interdisciplinary Coordination","award":["1635466"],"award-info":[{"award-number":["1635466"]}]},{"name":"Lewis and Clark Fund for Exploration and Field Research in Astrobiology"},{"DOI":"10.13039\/100000192","name":"National Oceanic and Atmospheric Administration","doi-asserted-by":"publisher","award":["NA17OAR0110212"],"award-info":[{"award-number":["NA17OAR0110212"]}],"id":[{"id":"10.13039\/100000192","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026,1,14]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>The deep ocean hosts autochthonous pressure-adapted microorganisms that are unique to this environment, as well as allochthonous pressure-sensitive members transported from shallow depths by vertical advection and particle-sinking. However, conventional sampling instruments decompress and warm deep-sea samples during retrieval, potentially altering microbial properties when studied ex situ. Here, we assess this potential sampling bias by comparing seawater microbial communities collected with or without measures aimed at minimizing pressure and temperature effects. When compared to samples collected under pressurized conditions, conventional sampling (using Niskin bottles) was found to affect prokaryotic cells retrieved by reducing their total numbers, diminishing protein synthesis activity (&amp;gt;10%), and also causing overall shifts in the community composition. The most significant compositional change was a &amp;gt;20% decrease in metagenomic archaeal representation (TACK-group\/Thaumarchaeota\/Nitrososphaerota). Deep-sea bacterial groups had mixed responses to preserving pressure during retrieval, with some groups exhibiting higher representation when samples were maintained pressurized (e.g. members of the family Pelagibacteraceae, unclassified Thiotricales, Thioglobaceae, and Chitinophagaceae), whereas others increased their representation when decompressed (e.g. Burkholderiaceae, Comamonadaceae, and Oxalobacteraceae). This study reveals the existence of bias introduced by the complete decompression of samples retrieved with traditional instrumentation, as well as a decrease in overall bacterial activity when samples are completely decompressed during retrieval. Additionally, incubations lasting for &amp;gt;24\u00a0h were shown to transform the original prokaryotic community composition. Precautions addressing these effects are necessary to enhance the reliability of ex situ measurements and improve our understanding of deep-sea microbial ecology and biogeochemistry.<\/jats:p>","DOI":"10.1093\/ismejo\/wrag064","type":"journal-article","created":{"date-parts":[[2026,7,2]],"date-time":"2026-07-02T18:39:41Z","timestamp":1783017581000},"source":"Crossref","is-referenced-by-count":1,"title":["Minimizing decompression and warming during deep seawater collection increases abundance and activity of autochthonous bacteria and archaea"],"prefix":"10.1093","volume":"20","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0816-8580","authenticated-orcid":false,"given":"Alvaro M","family":"Plominsky","sequence":"first","affiliation":[{"name":"Marine Biology Research Division, Scripps Institution of Oceanography, University of California San Diego , La Jolla, CA 92037 ,","place":["United States"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Logan 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,","place":["Chile"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7073-5190","authenticated-orcid":false,"given":"Sheila","family":"Podell","sequence":"additional","affiliation":[{"name":"Center for Marine Biotechnology and Biomedicine, Scripps Institution of Oceanography, University of California, San Diego , La Jolla, CA 92037 ,","place":["United States"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kelli K","family":"Mullane","sequence":"additional","affiliation":[{"name":"Marine Biology Research Division, Scripps Institution of Oceanography, University of California San Diego , La Jolla, CA 92037 ,","place":["United States"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David","family":"Casagrande","sequence":"additional","affiliation":[{"name":"Graduate School of Oceanography, University of Rhode Island , Narragansett, RI 02882 ,","place":["United 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University of California San Diego , La Jolla, CA 92037 ,","place":["United States"]},{"name":"Center for Marine Biotechnology and Biomedicine, Scripps Institution of Oceanography, University of California, San Diego , La Jolla, CA 92037 ,","place":["United States"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ronnie N","family":"Glud","sequence":"additional","affiliation":[{"name":"Nordcee and HADAL, Department of Biology, University of Southern Denmark , Odense M ,","place":["5230 M, Denmark"]},{"name":"Danish Institute for Advanced Study (DIAS), University of Southern Denmark , Odense M DK-5230 ,","place":["Denmark"]},{"name":"Tokyo University of Marine Science and Technology , Deptartment of Ocean Sciences, 4-5-7 Konan, Minato-ku, Tokyo 108-8477 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