{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,10]],"date-time":"2026-09-10T18:07:41Z","timestamp":1789063661189,"version":"build-2803163510"},"reference-count":70,"publisher":"Oxford University Press (OUP)","issue":"1","license":[{"start":{"date-parts":[[2026,8,19]],"date-time":"2026-08-19T00:00:00Z","timestamp":1787097600000},"content-version":"vor","delay-in-days":230,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026,1,14]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Streptococcus pneumoniae is a successful colonizer of the human upper respiratory tract; however, the mechanisms that enable its persistence in this nutrient-limited environment, with numerous immune mechanisms in place, remain enigmatic. Here, we examined how pneumococci adapt to upper respiratory tract conditions and how this affects host interactions. We measured intranasal metal ion and monosaccharide concentrations to create an in vivo-mimicking medium for studying pneumococcal adaptation. Growth in this medium was reduced compared to glucose-rich chemically defined media (CDM). Proteome analysis revealed a shift to galactose as the major carbohydrate source, and decreased levels of fatty acid biosynthesis proteins and pneumolysin, compared to other CDMs. Glycerophosphocholine accumulated extracellularly leading to decreased C-reactive protein and Immunoglobulin M binding to pneumococci. Pneumococci grown in in vivo-mimicking medium, compared to glucose-rich media, were more capable colonizers of primary epithelium and induced less epithelial cytokine release. Together, this shows how pneumococci adapt to the nutrient-limited respiratory environment, modulate epithelial cells, and evade humoral responses to facilitate persistent colonization.<\/jats:p>","DOI":"10.1093\/ismejo\/wrag213","type":"journal-article","created":{"date-parts":[[2026,8,15]],"date-time":"2026-08-15T12:47:27Z","timestamp":1786798047000},"source":"Crossref","is-referenced-by-count":0,"title":["<i>Streptococcus pneumoniae<\/i>\n                    adaptation to nutrient deprivation and immune modulation drives upper respiratory tract colonization"],"prefix":"10.1093","volume":"20","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2719-6408","authenticated-orcid":false,"given":"Daan W","family":"Arends","sequence":"first","affiliation":[{"name":"Centre for Immunology of Infectious Diseases and Vaccines, National Institute of Public Health and the Environment , 3720 BA Bilthoven ,","place":["The Netherlands"]},{"name":"Laboratory of Medical Immunology, Radboud University Medical Centre , 6500 HB Nijmegen ,","place":["The Netherlands"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kristin","family":"Surmann","sequence":"additional","affiliation":[{"name":"Department of Functional Genomics, Interfaculty Institute for Genetics and Functional Genomics, University Medicine Greifswald , 17475 Greifswald, Mecklenburg-Vorpommern ,","place":["Germany"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lucille F","family":"van Beek","sequence":"additional","affiliation":[{"name":"Laboratory of Medical Immunology, Radboud University Medical Centre , 6500 HB Nijmegen ,","place":["The Netherlands"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Robert S","family":"Jansen","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Radboud Institute for Biological and Environmental Sciences, Faculty of Science, Radboud University , 6525 AJ Nijmegen ,","place":["The Netherlands"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rob J","family":"Mesman","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Radboud Institute for Biological and Environmental Sciences, Faculty of Science, Radboud University , 6525 AJ Nijmegen ,","place":["The Netherlands"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jeroen D","family":"Langereis","sequence":"additional","affiliation":[{"name":"Laboratory of Medical Immunology, Radboud University Medical Centre , 6500 HB Nijmegen ,","place":["The Netherlands"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Monique","family":"van Scherpenzeel","sequence":"additional","affiliation":[{"name":"GlycoMScan , 6827 DG Arnhem ,","place":["The Netherlands"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Manuela Gesell","family":"Salazar","sequence":"additional","affiliation":[{"name":"Department of Functional Genomics, Interfaculty Institute for Genetics and Functional Genomics, University Medicine Greifswald , 17475 Greifswald, Mecklenburg-Vorpommern ,","place":["Germany"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5689-3448","authenticated-orcid":false,"given":"Uwe","family":"V\u00f6lker","sequence":"additional","affiliation":[{"name":"Department of Functional Genomics, Interfaculty Institute for Genetics and Functional Genomics, University Medicine Greifswald , 17475 Greifswald, Mecklenburg-Vorpommern ,","place":["Germany"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2103-6315","authenticated-orcid":false,"given":"Gerco","family":"den Hartog","sequence":"additional","affiliation":[{"name":"Centre for Immunology of Infectious Diseases and Vaccines, National Institute of Public Health and the Environment , 3720 BA Bilthoven ,","place":["The Netherlands"]},{"name":"Laboratory of Medical Immunology, Radboud University Medical Centre , 6500 HB Nijmegen ,","place":["The Netherlands"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2812-5895","authenticated-orcid":false,"given":"Marien I","family":"de Jonge","sequence":"additional","affiliation":[{"name":"Laboratory of Medical Immunology, Radboud University Medical Centre , 6500 HB Nijmegen ,","place":["The Netherlands"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2026,8,19]]},"reference":[{"key":"2026091013263546300_ref1","doi-asserted-by":"publisher","first-page":"55","DOI":"10.1093\/infdis\/132.1.55","article-title":"Spread of Streptococcus pneumoniae in families. 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