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We applied bioorthogonal non-canonical amino acid tagging coupled with fluorescence-activated cell sorting (BONCAT-FACS) and 16S rRNA gene sequencing to identify translationally active bacteria in early- (L-BSC) and late (D-BSC) successional cyanobacteria-dominated biocrusts subjected to 3\u00a0mm simulated rainfall under light and dark conditions. Our results reveal that only a small subset of the microbial community resumes activity within six hours, with higher active cell abundances in mature crusts. Microbial activity patterns were largely independent of light exposure and showed partial decoupling from total community composition, indicating that presence does not predict short-term function. These findings suggest that biocrust maturity shapes microbial activation dynamics and that functional responses to precipitation pulses are governed by a conserved pool of fast responders, informing predictions of dryland soil microbiome resilience under changing precipitation regimes.<\/jats:p>","DOI":"10.1093\/ismejo\/wrag231","type":"journal-article","created":{"date-parts":[[2026,9,4]],"date-time":"2026-09-04T11:27:16Z","timestamp":1788521236000},"source":"Crossref","is-referenced-by-count":1,"title":["Rainfall-induced microbial resuscitation reveals functional decoupling across biocrust succession"],"prefix":"10.1093","volume":"20","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5324-2033","authenticated-orcid":false,"given":"Ra\u00fal","family":"Rom\u00e1n","sequence":"first","affiliation":[{"name":"Department of Ecosystem Science and Management, The Pennsylvania State University, University Park , PA 16802 ,","place":["United States"]},{"name":"Instituto Multidisciplinar para el Estudio del Medio Ram\u00f3n Margalef (IMEM), Universidad de Alicante , Alicante, 03690 ,","place":["Spain"]},{"name":"Department of Agronomy, University of Almer\u00eda , Almer\u00eda, 04120 ,","place":["Spain"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7434-4856","authenticated-orcid":false,"given":"Fernando T","family":"Maestre","sequence":"additional","affiliation":[{"name":"Environmental Science and Engineering, Biological and Environmental Science and Engineering Division, King Abdullah University of Science and Technology , Thuwal, 23955-6900 ,","place":["Kingdom of Saudi Arabia"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3947-2529","authenticated-orcid":false,"given":"Estelle","family":"Couradeau","sequence":"additional","affiliation":[{"name":"Department of Ecosystem Science and Management, The Pennsylvania State University, University Park , PA 16802 ,","place":["United States"]},{"name":"The Huck Institutes of Life Sciences, The Pennsylvania State University , PA 16802 ,","place":["United States"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2026,9,4]]},"reference":[{"key":"2026092111373121100_ref1","doi-asserted-by":"publisher","first-page":"397","DOI":"10.1146\/annurev-ecolsys-120213-091650","article-title":"A multiscale, hierarchical model of pulse dynamics in arid-land ecosystems","volume":"45","author":"Collins","year":"2014","journal-title":"Annu Rev Ecol Evol Syst"},{"key":"2026092111373121100_ref2","doi-asserted-by":"publisher","first-page":"119","DOI":"10.1038\/nrmicro2504","article-title":"Microbial seed banks: the ecological and evolutionary implications of dormancy","volume":"9","author":"Lennon","year":"2011","journal-title":"Nat Rev Microbiol"},{"key":"2026092111373121100_ref3","doi-asserted-by":"publisher","first-page":"409","DOI":"10.1146\/annurev-ecolsys-110617-062614","article-title":"Life in dry soils: effects of drought on soil microbial communities and processes","volume":"49","author":"Schimel","year":"2018","journal-title":"Annu Rev Ecol Evol Syst"},{"key":"2026092111373121100_ref4","first-page":"673","volume-title":"Climate Change 2021: The Physical Science Basis","author":"Canadell","year":"2021"},{"key":"2026092111373121100_ref5","doi-asserted-by":"publisher","first-page":"15684","DOI":"10.1073\/pnas.1516684112","article-title":"Increasing aridity reduces soil microbial diversity and abundance in global drylands","volume":"112","author":"Maestre","year":"2015","journal-title":"Proc Natl Acad Sci USA"},{"key":"2026092111373121100_ref6","doi-asserted-by":"publisher","first-page":"181","DOI":"10.1890\/1540-9295(2003)001[0181:TWAYFD]2.0.CO;2","article-title":"The world at your feet: desert biological soil crusts","volume":"1","author":"Belnap","year":"2003","journal-title":"Front Ecol Environ"},{"key":"2026092111373121100_ref7","doi-asserted-by":"publisher","first-page":"1768","DOI":"10.1111\/brv.12862","article-title":"What is a biocrust? 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