{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,13]],"date-time":"2026-10-13T12:50:59Z","timestamp":1791895859813,"version":"4.3.4"},"reference-count":0,"publisher":"Oxford University Press (OUP)","license":[{"start":{"date-parts":[[2026,10,13]],"date-time":"2026-10-13T00:00:00Z","timestamp":1791849600000},"content-version":"am","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Methane emissions from rice paddies are a major source of greenhouse gases, and silicate fertilizer (SF) derived from blast furnace slag has emerged as an environmentally friendly amendment that reduces methane release. SF modifies the soil redox environment by supplying electron acceptors such as iron, but the microbial mechanisms underlying methane mitigation remain unclear. Here, we combined metagenomic profiling, microbial isolation, and greenhouse experiments to show that SF consistently enriched Burkholderia vietnamiensis together with the methanotrophs in the rice rhizosphere. Inoculation of B. vietnamiensis strain HU01 alone reproduced the methane-suppressing effect of SF and increased methanotroph abundance. Cell-free culture supernatants of HU01 stimulated the growth of Methylococcus geothermalis and Methylocystis parvus through secreted, low-molecular-weight, heat-stable metabolites. Although metabolic modeling predicted primary metabolites such as L-glutamate and cobalamin as candidate cross-feeding factors, LC\u2013MS\/MS analysis of the highly active fractions indicated that the robust bioactivity was primarily driven by a chemically complex metabolite mixture rather than these ubiquitous single compounds. These results provide direct evidence that enrichment of a single heterotrophic species can enhance methane oxidation by stimulating methanotroph activity through metabolite exchange. B. vietnamiensis HU01 represents a promising microbial resource for methane mitigation and illustrates how targeted heterotroph\u2013methanotroph interactions can be leveraged for climate-smart rice cultivation.<\/jats:p>","DOI":"10.1093\/ismejo\/wrag255","type":"journal-article","created":{"date-parts":[[2026,10,13]],"date-time":"2026-10-13T11:39:38Z","timestamp":1791891578000},"source":"Crossref","is-referenced-by-count":0,"title":["Silicate fertilizer mitigates methane emissions from rice paddies by enriching\n                    <i>Burkholderia<\/i>\n                    to modulate microbial interactions"],"prefix":"10.1093","author":[{"given":"Seonghan","family":"Jang","sequence":"first","affiliation":[{"name":"Infectious Disease Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB) , 125 Gwahak-ro, Yuseong-gu, Daejeon 34141 ,","place":["South Korea"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hyeonu","family":"Yang","sequence":"additional","affiliation":[{"name":"Infectious Disease Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB) , 125 Gwahak-ro, Yuseong-gu, Daejeon 34141 ,","place":["South Korea"]},{"name":"Department of Biosystems and Bioengineering, KRIBB School of Biotechnology, Korea University of Science and Technology (UST) , 217 Gajeong-ro, Yuseong-gu, Daejeon 34113 ,","place":["South Korea"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"SuYeon","family":"Lee","sequence":"additional","affiliation":[{"name":"Infectious Disease Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB) , 125 Gwahak-ro, Yuseong-gu, Daejeon 34141 ,","place":["South Korea"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"PilJoo","family":"Kim","sequence":"additional","affiliation":[{"name":"Institute of Agriculture and Life Sciences, Gyeongsang National University , Jinju 660701 ,","place":["South Korea"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bong-Sik","family":"Yun","sequence":"additional","affiliation":[{"name":"Division of Biotechnology and Advanced Institute of Environment and Bioscience, College of Environmental and Bioresource Sciences, Jeonbuk National University , Gobong-ro 79, Iksan 54596 ,","place":["South Korea"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7575-1250","authenticated-orcid":false,"given":"Dajeong","family":"Kim","sequence":"additional","affiliation":[{"name":"Infectious Disease Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB) , 125 Gwahak-ro, Yuseong-gu, Daejeon 34141 ,","place":["South Korea"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7276-1189","authenticated-orcid":false,"given":"Choong-Min","family":"Ryu","sequence":"additional","affiliation":[{"name":"Infectious Disease Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB) , 125 Gwahak-ro, Yuseong-gu, Daejeon 34141 ,","place":["South Korea"]},{"name":"Department of Biosystems and Bioengineering, KRIBB School of Biotechnology, Korea University of Science and Technology (UST) , 217 Gajeong-ro, Yuseong-gu, Daejeon 34113 ,","place":["South Korea"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2026,10,13]]},"container-title":["The ISME Journal"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/academic.oup.com\/ismej\/advance-article-pdf\/doi\/10.1093\/ismejo\/wrag255\/71907730\/wrag255.pdf","content-type":"application\/pdf","content-version":"am","intended-application":"syndication"},{"URL":"https:\/\/academic.oup.com\/ismej\/advance-article-pdf\/doi\/10.1093\/ismejo\/wrag255\/71907730\/wrag255.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,10,13]],"date-time":"2026-10-13T12:12:57Z","timestamp":1791893577000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/ismej\/advance-article\/doi\/10.1093\/ismejo\/wrag255\/8914322"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,10,13]]},"references-count":0,"URL":"https:\/\/doi.org\/10.1093\/ismejo\/wrag255","relation":{},"ISSN":["1751-7362","1751-7370"],"issn-type":[{"value":"1751-7362","type":"print"},{"value":"1751-7370","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,10,13]]},"article-number":"wrag255"}}