{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,25]],"date-time":"2026-08-25T19:31:43Z","timestamp":1787686303272,"version":"build-2784847793"},"reference-count":20,"publisher":"Oxford University Press (OUP)","issue":"6","license":[{"start":{"date-parts":[[2020,2,28]],"date-time":"2020-02-28T00:00:00Z","timestamp":1582848000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["31772792"],"award-info":[{"award-number":["31772792"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Special Project for the Cultivation of Major Projects in International Science and Technology Cooperation","award":["2019SCAUGH02"],"award-info":[{"award-number":["2019SCAUGH02"]}]},{"name":"Graduate Student Oversea Study Program","award":["2017LHPY029"],"award-info":[{"award-number":["2017LHPY029"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2020,6,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:sec>\n                  <jats:title>Objectives<\/jats:title>\n                  <jats:p>To clarify the transmission mechanism of the blaCTX-M-64 gene between Escherichia coli and Salmonella isolates from food animals.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Methods<\/jats:title>\n                  <jats:p>A total of 329 E. coli and 60 Salmonella isolates collected from food animals in 2016 were screened for the presence of blaCTX-M-64 genes. The blaCTX-M-64-positive isolates were typed and plasmid and chromosome DNA was sequenced to determine the genetic context of blaCTX-M-64 and the plasmid types present.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Results<\/jats:title>\n                  <jats:p>The blaCTX-M-64 gene was identified in only three E. coli isolates but was the predominant gene in the Salmonella isolates (n\u2009=\u20099). These 12 CTX-M-64-positive isolates were all resistant to ampicillin, cefotaxime, ceftiofur, ceftriaxone, ceftazidime and florfenicol and 9 were resistant to ciprofloxacin. The blaCTX-M-64 gene was located on transferable IncI2 plasmids and an IncHI2 plasmid in three E. coli and one Salmonella isolate, respectively. The remaining eight Salmonella isolates contained blaCTX-M-64 integrated into the chromosome. Different genetic contexts of blaCTX-M-64 genes were found among the 12 isolates: ISEcp1-blaCTX-M-64-orf477-A\/C on IncI2 plasmids of 3 E. coli isolates; \u0394ISEcp1-blaCTX-M-64-orf477-A\/C in the chromosome of 1 Salmonella isolate; and ISEcp1-blaCTX-M-64-orf477 on the IncHI2 plasmid and chromosome of 8 Salmonella isolates.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Conclusions<\/jats:title>\n                  <jats:p>To the best of our knowledge, this is the first report of chromosomally encoded CTX-M-64 in Salmonella isolates. ISEcp1-mediated transposition is likely to be responsible for the spread of blaCTX-M-64 between different plasmids and chromosomes in Enterobacteriaceae especially E. coli and Salmonella.<\/jats:p>\n               <\/jats:sec>","DOI":"10.1093\/jac\/dkaa044","type":"journal-article","created":{"date-parts":[[2020,1,30]],"date-time":"2020-01-30T12:11:46Z","timestamp":1580386306000},"page":"1424-1427","source":"Crossref","is-referenced-by-count":13,"title":["Transmission of plasmid-borne and chromosomal blaCTX-M-64 among Escherichia coli and Salmonella isolates from food-producing animals via ISEcp1-mediated transposition"],"prefix":"10.1093","volume":"75","author":[{"given":"Qiu-Yun","family":"Zhao","sequence":"first","affiliation":[{"name":"National Risk Assessment Laboratory for Antimicrobial Resistance of Animal Original Bacteria, College of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pin-Xian","family":"Chen","sequence":"first","affiliation":[{"name":"National Risk Assessment Laboratory for Antimicrobial Resistance of Animal Original Bacteria, College of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ling","family":"Yang","sequence":"first","affiliation":[{"name":"National Risk Assessment Laboratory for Antimicrobial Resistance of Animal Original Bacteria, College of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Run-Mao","family":"Cai","sequence":"first","affiliation":[{"name":"National Risk Assessment Laboratory for Antimicrobial Resistance of Animal Original Bacteria, College of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jia-Hang","family":"Zhu","sequence":"first","affiliation":[{"name":"National Risk Assessment Laboratory for Antimicrobial Resistance of Animal Original Bacteria, College of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Liang-Xing","family":"Fang","sequence":"first","affiliation":[{"name":"National Risk Assessment Laboratory for Antimicrobial Resistance of Animal Original Bacteria, College of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, China"},{"name":"Guangdong Key Laboratory for Veterinary Drug Development and Safety Evaluation, College of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9169-7592","authenticated-orcid":false,"given":"Mark A","family":"Webber","sequence":"first","affiliation":[{"name":"Quadram Institute Bioscience, Norwich Research Park, Norwich, Norfolk, NR4 7UA, UK"},{"name":"Norwich Medical School, University of East Anglia, Norwich Research Park, Norwich, Norfolk, NR4 7TJ, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hong-Xia","family":"Jiang","sequence":"first","affiliation":[{"name":"National Risk Assessment Laboratory for Antimicrobial Resistance of Animal Original Bacteria, College of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, China"},{"name":"Guangdong Key Laboratory for Veterinary Drug Development and Safety Evaluation, College of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2020,2,28]]},"reference":[{"key":"2020051504584294900_dkaa044-B1","doi-asserted-by":"crossref","first-page":"1475","DOI":"10.1111\/j.1469-0691.2010.03127.x","article-title":"High prevalence of bla(CTX-M) extended-spectrum \u03b2-lactamase genes in Escherichia coli isolates from pets and emergence of CTX-M-64 in China","volume":"16","author":"Sun","year":"2010","journal-title":"Clin Microbiol Infect"},{"key":"2020051504584294900_dkaa044-B2","doi-asserted-by":"crossref","first-page":"4068","DOI":"10.1128\/AAC.00541-13","article-title":"CTX-M-123, a novel hybrid of the CTX-M-1 and CTX-M-9 group \u03b2-lactamases recovered from Escherichia coli isolates in China","volume":"57","author":"He","year":"2013","journal-title":"Antimicrob Agents Chemother"},{"key":"2020051504584294900_dkaa044-B3","doi-asserted-by":"crossref","first-page":"1387","DOI":"10.1089\/fpd.2010.0581","article-title":"Dissemination of cefotaxime-M-producing Escherichia coli isolates in poultry farms, but not swine farms, in China","volume":"7","author":"Li","year":"2010","journal-title":"Foodborne Pathog Dis"},{"key":"2020051504584294900_dkaa044-B4","doi-asserted-by":"crossref","first-page":"2081","DOI":"10.1093\/jac\/dku126","article-title":"CTX-M-137, a hybrid of CTX-M-14-like and CTX-M-15-like \u03b2-lactamases identified in an Escherichia coli clinical isolate","volume":"69","author":"Tian","year":"2014","journal-title":"J Antimicrob Chemother"},{"key":"2020051504584294900_dkaa044-B5","doi-asserted-by":"crossref","first-page":"69","DOI":"10.1128\/AAC.00227-08","article-title":"Novel chimeric \u03b2-lactamase CTX-M-64, a hybrid of CTX-M-15-like and CTX-M-14 \u03b2-lactamases, found in a Shigella sonnei strain resistant to various oxyimino-cephalosporins, including ceftazidime","volume":"53","author":"Nagano","year":"2009","journal-title":"Antimicrob Agents Chemother"},{"key":"2020051504584294900_dkaa044-B6","doi-asserted-by":"crossref","first-page":"148","DOI":"10.1016\/j.diagmicrobio.2015.02.010","article-title":"Prevalence and characterization of hybrid blaCTX-M among Escherichia coli isolates from livestock and other animals","volume":"82","author":"Ho","year":"2015","journal-title":"Diagn Micr Infect Dis"},{"key":"2020051504584294900_dkaa044-B7","doi-asserted-by":"crossref","first-page":"1371","DOI":"10.1128\/AAC.04158-14","article-title":"Detection of CTX-M-64 in Escherichia coli isolates from human patients in China","volume":"59","author":"Gu","year":"2015","journal-title":"Antimicrob Agents Chemother"},{"key":"2020051504584294900_dkaa044-B8","doi-asserted-by":"crossref","first-page":"1136","DOI":"10.1111\/j.1365-2958.2006.05172.x","article-title":"Sex and virulence in Escherichia coli: an evolutionary perspective","volume":"60","author":"Wirth","year":"2006","journal-title":"Mol Microbiol"},{"key":"2020051504584294900_dkaa044-B9","year":"2016"},{"key":"2020051504584294900_dkaa044-B10","year":"2013"},{"key":"2020051504584294900_dkaa044-B11","doi-asserted-by":"crossref","first-page":"219","DOI":"10.1016\/j.mimet.2005.03.018","article-title":"Identification of plasmids by PCR-based replicon typing","volume":"63","author":"Carattoli","year":"2005","journal-title":"J Microbiol Meth"},{"key":"2020051504584294900_dkaa044-B12","doi-asserted-by":"crossref","first-page":"3355","DOI":"10.1128\/JB.177.11.3355-3357.1995","article-title":"I-CeuI reveals conservation of the genome of independent strains of Salmonella typhimurium","volume":"177","author":"Liu","year":"1995","journal-title":"J Bacteriol"},{"key":"2020051504584294900_dkaa044-B13","doi-asserted-by":"crossref","first-page":"334","DOI":"10.1093\/jac\/dky441","article-title":"Clonal expansion and horizontal transmission of epidemic F2:A1:B1 plasmids involved in co-spread of rmtB with qepA and blaCTX-M-27 in extensively drug-resistant Salmonella enterica serovar Indiana isolates","volume":"74","author":"Fang","year":"2019","journal-title":"J Antimicrob Chemother"},{"key":"2020051504584294900_dkaa044-B14","doi-asserted-by":"crossref","first-page":"5026","DOI":"10.1128\/AAC.00775-15","article-title":"Dissemination of IncI2 plasmids that harbor the blaCTX-M element among clinical Salmonella isolates","volume":"59","author":"Wong","year":"2015","journal-title":"Antimicrob Agents Chemother"},{"key":"2020051504584294900_dkaa044-B15","doi-asserted-by":"crossref","first-page":"1808","DOI":"10.1128\/AAC.00451-08","article-title":"Chromosomal integration of the extended-spectrum-lactamase gene blaCTX-M-15 in Salmonella enterica serotype Concord isolates from internationally adopted children","volume":"53","author":"Fabre","year":"2009","journal-title":"Antimicrob Agents Chemother"},{"key":"2020051504584294900_dkaa044-B16","doi-asserted-by":"crossref","first-page":"53","DOI":"10.1016\/j.jgar.2018.11.006","article-title":"Characterisation of chromosomally-located blaCTX-M and its surrounding sequence in CTX-M-type extended-spectrum \u03b2-lactamase-producing Escherichia coli isolates","volume":"17","author":"Hamamoto","year":"2019","journal-title":"J Glob Antimicrob Res"},{"key":"2020051504584294900_dkaa044-B17","doi-asserted-by":"crossref","first-page":"4895","DOI":"10.1128\/AAC.48.12.4895-4897.2004","article-title":"Chromosome-encoded CTX-M-3 from Kluyvera ascorbata: a possible origin of plasmid-borne CTX-M-1-derived cefotaximases","volume":"48","author":"Rodr\u00edguez","year":"2004","journal-title":"Antimicrob Agents Chemother"},{"key":"2020051504584294900_dkaa044-B18","doi-asserted-by":"crossref","first-page":"1093","DOI":"10.1128\/AAC.00258-11","article-title":"Chromosomal integration and location on IncT plasmids of the blaCTX-M-2 gene in Proteus mirabilis clinical isolates","volume":"56","author":"Harada","year":"2012","journal-title":"Antimicrob Agents Chemother"},{"key":"2020051504584294900_dkaa044-B19","doi-asserted-by":"crossref","first-page":"e01740","DOI":"10.1128\/AEM.01740-19","article-title":"Wastewater as a probable environmental reservoir of extended-spectrum \u03b2-lactamase genes: detection of chimeric \u03b2-lactamases CTX-M-64 and CTX-M-123","volume":"85","author":"Tanaka","year":"2019","journal-title":"Appl Environ Microbiol"},{"key":"2020051504584294900_dkaa044-B20","doi-asserted-by":"crossref","first-page":"4464","DOI":"10.1128\/AAC.00501-15","article-title":"bla\n            CTX-M-1\/9\/1 hybrid genes may have been generated from blaCTX-M-15 on an IncI2 plasmid","volume":"59","author":"Liu","year":"2015","journal-title":"Antimicrob Agents Chemother"}],"container-title":["Journal of Antimicrobial Chemotherapy"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/academic.oup.com\/jac\/article-pdf\/75\/6\/1424\/33213889\/dkaa044.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"http:\/\/academic.oup.com\/jac\/article-pdf\/75\/6\/1424\/33213889\/dkaa044.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2020,5,16]],"date-time":"2020-05-16T20:30:27Z","timestamp":1589661027000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/jac\/article\/75\/6\/1424\/5766079"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,2,28]]},"references-count":20,"journal-issue":{"issue":"6","published-online":{"date-parts":[[2020,2,28]]},"published-print":{"date-parts":[[2020,6,1]]}},"URL":"https:\/\/doi.org\/10.1093\/jac\/dkaa044","relation":{},"ISSN":["0305-7453","1460-2091"],"issn-type":[{"value":"0305-7453","type":"print"},{"value":"1460-2091","type":"electronic"}],"subject":[],"published-other":{"date-parts":[[2020,6]]},"published":{"date-parts":[[2020,2,28]]}}}