{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,9]],"date-time":"2026-10-09T20:41:03Z","timestamp":1791578463363,"version":"4.3.4"},"reference-count":27,"publisher":"Oxford University Press (OUP)","issue":"7","license":[{"start":{"date-parts":[[2020,4,17]],"date-time":"2020-04-17T00:00:00Z","timestamp":1587081600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"funder":[{"DOI":"10.13039\/501100003243","name":"Ministry of Health of the Czech Republic","doi-asserted-by":"publisher","award":["NV 17-31269A"],"award-info":[{"award-number":["NV 17-31269A"]}],"id":[{"id":"10.13039\/501100003243","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2020,7,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:sec>\n                  <jats:title>Objectives<\/jats:title>\n                  <jats:p>Terbinafine resistance is increasingly reported in Trichophyton, rendering susceptibility testing particularly important in non-responding cases. We performed a multicentre evaluation of six EUCAST-based methods.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Methods<\/jats:title>\n                  <jats:p>Ten laboratories susceptibility tested terbinafine, itraconazole, voriconazole and amorolfine against a blinded panel of 38 terbinafine WT and target gene mutant isolates. E.Def 9.3.1 modifications included: medium with\/without addition of chloramphenicol and cycloheximide (CC), incubation at 25\u00b0C to 28\u00b0C for 5\u20137\u2009days and three MIC endpoints [visually and spectrophotometrically (90%\/50% inhibition)], generating 7829 MICs. Quality control (QC) strains were Aspergillus flavus ATCC 204304 and CNM-CM1813. Eyeball, ECOFFinder (where ECOFF stands for epidemiological cut-off) and derivatization WT upper limits (WT-ULs), very major errors (VMEs; mutants with MICs \u2264WT-ULs) and major errors (MEs; WT isolates with MICs &amp;gt;WT-ULs) were determined.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Results<\/jats:title>\n                  <jats:p>MICs fell within the QC ranges for ATCC 204304\/CNM-CM1813 for 100%\/96% (voriconazole) and 84%\/84% (itraconazole), respectively. Terbinafine MICs fell within 0.25\u20131\u2009mg\/L for 96%\/92%, suggesting high reproducibility. Across the six methods, the number of terbinafine MEs varied from 2 to 4 (2.6%\u20135.2%) for Trichophyton rubrum and from 0 to 2 (0%\u20132.0%) for Trichophyton interdigitale. Modes for WT and mutant populations were at least seven 2-fold dilutions apart in all cases. Excluding one I121M\/V237I T. rubrum mutant and two mixed WT\/mutant T. interdigitale specimens, the numbers of VMEs were as follows: T. rubrum: CC visual, 1\/67 (1.5%); CC spectrophotometric 90% inhibition, 3\/59 (5.1%); and CC spectrophotometric 50% inhibition, 1\/67 (1.5%); and T. interdigitale: none. Voriconazole and amorolfine MICs were quite uniform, but trailing growth complicated determination of itraconazole visual and spectrophotometric 90% inhibition MIC.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Conclusions<\/jats:title>\n                  <jats:p>Although none of the laboratories was experienced in dermatophyte testing, error rates were low. We recommend the CC spectrophotometric 50% inhibition method and provide QC ranges and WT-ULs for WT\/non-WT classification.<\/jats:p>\n               <\/jats:sec>","DOI":"10.1093\/jac\/dkaa111","type":"journal-article","created":{"date-parts":[[2020,3,6]],"date-time":"2020-03-06T12:24:43Z","timestamp":1583497483000},"page":"1807-1819","source":"Crossref","is-referenced-by-count":67,"title":["Multicentre validation of a EUCAST method for the antifungal susceptibility testing of microconidia-forming dermatophytes"],"prefix":"10.1093","volume":"75","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4747-0144","authenticated-orcid":false,"given":"Maiken Cavling","family":"Arendrup","sequence":"first","affiliation":[{"name":"Unit for Mycology, Statens Serum Institut, Copenhagen, Denmark"},{"name":"Department of Clinical Microbiology, Rigshospitalet, Copenhagen, Denmark"},{"name":"Department of Clinical Medicine, Copenhagen University, Copenhagen, Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Karin Meinike","family":"J\u00f8rgensen","sequence":"first","affiliation":[{"name":"Unit for Mycology, Statens Serum Institut, Copenhagen, Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7901-8355","authenticated-orcid":false,"given":"Jesus","family":"Guinea","sequence":"first","affiliation":[{"name":"Clinical Microbiology and Infectious Diseases, Hospital General Universitario Gregorio Mara\u00f1\u00f3n, Madrid, Spain and Instituto de Investigaci\u00f3n Sanitaria Gregorio Mara\u00f1\u00f3n, Madrid, Spain"},{"name":"CIBER Enfermedades Respiratorias-CIBERES (CB06\/06\/0058), Madrid, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Katrien","family":"Lagrou","sequence":"first","affiliation":[{"name":"Department of Microbiology, Immunology and Transplantation, KU Leuven, Leuven, Belgium"},{"name":"Department of Laboratory Medicine and National Reference Centre for Mycosis, University Hospitals Leuven, Leuven, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Erja","family":"Chryssanthou","sequence":"first","affiliation":[{"name":"Department of Clinical Microbiology, Karolinska University Hospital, Stockholm, Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marie-Pierre","family":"Hayette","sequence":"first","affiliation":[{"name":"Department of Clinical Microbiology, Centre for Interdisciplinary Research on Medicines, University of Li\u00e8ge, Li\u00e8ge, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Francesco","family":"Barchiesi","sequence":"first","affiliation":[{"name":"Dipartimento di Scienze Biomediche e Sanit\u00e0 Pubblica, Universit\u00e0 Politecnica delle Marche, Ancona, Italy"},{"name":"Malattie Infettive, Ospedali Riuniti Marche Nord, Pesaro, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cornelia","family":"Lass-Fl\u00f6rl","sequence":"first","affiliation":[{"name":"Institute of Hygiene and Medical Microbiology, Medical University of Innsbruck, Innsbruck, Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Petr","family":"Hamal","sequence":"first","affiliation":[{"name":"Department of Microbiology, University Hospital, Olomouc, Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eric","family":"Dannaoui","sequence":"first","affiliation":[{"name":"Parasitology-Mycology Unit, Microbiology Department, Georges Pompidou European Hospital, University of Paris, Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anuradha","family":"Chowdhary","sequence":"first","affiliation":[{"name":"Department of Medical Mycology, Vallabhbhai Patel Chest Institute, University of Delhi, Delhi, 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