{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,27]],"date-time":"2026-09-27T00:55:22Z","timestamp":1790470522466,"version":"4.1.0"},"reference-count":29,"publisher":"Oxford University Press (OUP)","issue":"9","license":[{"start":{"date-parts":[[2020,5,22]],"date-time":"2020-05-22T00:00:00Z","timestamp":1590105600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"funder":[{"name":"Universit\u00e9 de Reims Champagne-Ardenne","award":["UMR-S 1250"],"award-info":[{"award-number":["UMR-S 1250"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2020,9,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:sec>\n                  <jats:title>Background<\/jats:title>\n                  <jats:p>Quinolone resistance (QR) is one component of the MDR emerging in Escherichia coli and is of particular concern given the widespread use of fluoroquinolones.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Objectives<\/jats:title>\n                  <jats:p>To characterize the QR phenotypes and genotypes in E. coli responsible for bloodstream infections and to propose molecular determinants that could be targeted to predict ciprofloxacin resistance.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Methods<\/jats:title>\n                  <jats:p>E. coli isolates from blood cultures in three French hospitals were studied for quinolone MICs and characterization of genotypic QR determinants (QRg).<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Results<\/jats:title>\n                  <jats:p>Among 507 isolates tested for MICs, 148 (29.2%) were resistant to quinolones based on EUCAST breakpoints and 143 (28.2%) harboured at least one QRg. QRg were mainly mutations in the QRDR (138 isolates, 27.2%), with 55.8% of these isolates carrying at least three QRDR mutations. gyrA mutations predominated (92.8%) followed by parC (61.6%), parE (32.6%) and gyrB (1.4%) mutations. Only 4.7% of the isolates harboured a plasmid-mediated quinolone resistance (PMQR) gene: aac(6\u2032)-Ib-cr (60.0%) or qnr (qnrS, qnrB) (32.0%). For the first time in France, we reported the qepA4 allele of the plasmid-encoded efflux pump QepA. Only five isolates carried PMQR without a QRDR mutation. The positive predictive value (PPV) for ciprofloxacin resistance was 100% for any QRg and 99.2% for gyrA mutations specifically.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Conclusions<\/jats:title>\n                  <jats:p>QR observed in E. coli isolates involved in bloodstream infections is still mainly due to QRDR mutations, especially at codons GyrA83\/87, which could be used as a molecular target to rapidly detect resistance.<\/jats:p>\n               <\/jats:sec>","DOI":"10.1093\/jac\/dkaa189","type":"journal-article","created":{"date-parts":[[2020,4,23]],"date-time":"2020-04-23T11:07:39Z","timestamp":1587640059000},"page":"2466-2470","source":"Crossref","is-referenced-by-count":13,"title":["Phenotypic and genotypic quinolone resistance in Escherichia coli underlining GyrA83\/87 mutations as a target to detect ciprofloxacin resistance"],"prefix":"10.1093","volume":"75","author":[{"given":"Ana\u00eblle","family":"Muggeo","sequence":"first","affiliation":[{"name":"Universit\u00e9 de Reims-Champagne-Ardenne, SFR CAP-Sant\u00e9, Inserm UMR-S 1250 P3Cell, 51097, Reims, France"},{"name":"Laboratoire de Bact\u00e9riologie-Virologie-Hygi\u00e8ne Hospitali\u00e8re-Parasitologie-Mycologie, CHU Reims, H\u00f4pital Robert Debr\u00e9, Reims, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Emmanuelle","family":"Cambau","sequence":"additional","affiliation":[{"name":"Universit\u00e9 de Paris, Inserm IAME UMR 1137, Paris, France and APHP Lariboisi\u00e8re-Fernand Widal, Laboratoire de Bact\u00e9riologie, Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marl\u00e8ne","family":"Amara","sequence":"additional","affiliation":[{"name":"Service de Biologie, Unit\u00e9 de Microbiologie, Centre Hospitalier de Versailles, Le Chesnay, 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France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hanaa","family":"Benmansour","sequence":"additional","affiliation":[{"name":"Universit\u00e9 de Paris, Inserm IAME UMR 1137, Paris, France and APHP Lariboisi\u00e8re-Fernand Widal, Laboratoire de Bact\u00e9riologie, Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christophe","family":"de Champs","sequence":"additional","affiliation":[{"name":"Universit\u00e9 de Reims-Champagne-Ardenne, SFR CAP-Sant\u00e9, Inserm UMR-S 1250 P3Cell, 51097, Reims, France"},{"name":"Laboratoire de Bact\u00e9riologie-Virologie-Hygi\u00e8ne Hospitali\u00e8re-Parasitologie-Mycologie, CHU Reims, H\u00f4pital Robert Debr\u00e9, Reims, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3795-0398","authenticated-orcid":false,"given":"Thomas","family":"Guillard","sequence":"additional","affiliation":[{"name":"Universit\u00e9 de Reims-Champagne-Ardenne, SFR 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