{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T13:07:32Z","timestamp":1790687252143,"version":"4.1.0"},"reference-count":24,"publisher":"Oxford University Press (OUP)","issue":"1","license":[{"start":{"date-parts":[[2020,10,9]],"date-time":"2020-10-09T00:00:00Z","timestamp":1602201600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"funder":[{"name":"Assistance Publique-H\u00f4pitaux de Paris"},{"name":"University Paris-Saclay, the Laboratory of Excellence in Research on Medication and Innovative Therapeutics"},{"DOI":"10.13039\/501100001665","name":"French National Research Agency","doi-asserted-by":"publisher","award":["ANR-10-LABX-33"],"award-info":[{"award-number":["ANR-10-LABX-33"]}],"id":[{"id":"10.13039\/501100001665","id-type":"DOI","asserted-by":"publisher"}]},{"name":"EIT health for the AMR-Detectool"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2021,1,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:sec>\n                  <jats:title>Background<\/jats:title>\n                  <jats:p>VRE are nosocomial pathogens with an increasing incidence in recent decades. Rapid detection is crucial to reduce their spread and prevent infections and outbreaks.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Objectives<\/jats:title>\n                  <jats:p>To evaluate a lateral flow immunoassay (LFIA) (called NG-Test VanA) for the rapid and reliable detection of VanA-producing VRE (VanA-VRE) from colonies and broth.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Methods<\/jats:title>\n                  <jats:p>NG-Test VanA was validated on 135 well-characterized enterococcal isolates grown on Mueller\u2013Hinton (MH) agar (including 40 VanA-VRE). Different agar plates and culture broths widely used in routine laboratories for culture of enterococci were tested.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Results<\/jats:title>\n                  <jats:p>All 40 VanA-VRE clinical isolates were correctly detected in less than 15\u2009min irrespective of the species expressing the VanA ligase and the medium used for bacterial growth. No cross-reaction was observed with any other clinically relevant ligases (VanB, C1, C2, D, E, G, L, M and N). Overall, the sensitivity and specificity of the assay were 100% for VanA-VRE grown on MH agar plates. NG-Test VanA accurately detects VanA-VRE irrespective of the culture medium (agar and broth). Band intensity was increased when using bacteria grown on vancomycin-containing culture media or on MH close to the vancomycin disc as a consequence of VanA induction. The limit of detection of the assay was 6.3\u205f\u00d7\u205f106 cfu per test with bacteria grown on MH plates and 4.9\u205f\u00d7\u205f105 cfu per test with bacteria grown on ChromID\u00ae VRE plates.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Conclusions<\/jats:title>\n                  <jats:p>NG-Test VanA is efficient, rapid and easy to implement in the routine workflow of a clinical microbiology laboratory for the confirmation of VanA-VRE.<\/jats:p>\n               <\/jats:sec>","DOI":"10.1093\/jac\/dkaa413","type":"journal-article","created":{"date-parts":[[2020,9,11]],"date-time":"2020-09-11T19:10:50Z","timestamp":1599851450000},"page":"146-151","source":"Crossref","is-referenced-by-count":16,"title":["Development and validation of a lateral flow immunoassay for rapid detection of VanA-producing enterococci"],"prefix":"10.1093","volume":"76","author":[{"given":"Saoussen","family":"Oueslati","sequence":"first","affiliation":[{"name":"Team ReSIST, INSERM U1184, School of Medicine, Universit\u00e9 Paris-Saclay, LabEx LERMIT, Le Kremlin-Bic\u00eatre, France"},{"name":"Bacteriology-Hygiene Unit, Assistance Publique\/H\u00f4pitaux de Paris, Bic\u00eatre Hospital, Le Kremlin-Bic\u00eatre, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Herv\u00e9","family":"Volland","sequence":"additional","affiliation":[{"name":"Universit\u00e9 Paris-Saclay, CEA, INRAE, D\u00e9partement M\u00e9dicaments et Technologies pour la Sant\u00e9, 91191 Gif-sur-Yvette, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4026-9912","authenticated-orcid":false,"given":"Vincent","family":"Cattoir","sequence":"additional","affiliation":[{"name":"Department of Clinical Microbiology and French National Reference Centre for Antibiotic Resistance: Glycopeptide-Resistant Enterococci, University Hospital, Rennes, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sandrine","family":"Bernabeu","sequence":"additional","affiliation":[{"name":"Team ReSIST, INSERM U1184, School of Medicine, Universit\u00e9 Paris-Saclay, LabEx LERMIT, Le Kremlin-Bic\u00eatre, France"},{"name":"Bacteriology-Hygiene Unit, Assistance Publique\/H\u00f4pitaux de Paris, Bic\u00eatre Hospital, Le Kremlin-Bic\u00eatre, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Delphine","family":"Girlich","sequence":"additional","affiliation":[{"name":"Team ReSIST, INSERM U1184, School of Medicine, Universit\u00e9 Paris-Saclay, LabEx LERMIT, Le Kremlin-Bic\u00eatre, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ducan","family":"Dulac","sequence":"additional","affiliation":[{"name":"Universit\u00e9 Paris-Saclay, CEA, INRAE, D\u00e9partement M\u00e9dicaments et Technologies pour la Sant\u00e9, 91191 Gif-sur-Yvette, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marc","family":"Plaisance","sequence":"additional","affiliation":[{"name":"Universit\u00e9 Paris-Saclay, CEA, INRAE, D\u00e9partement M\u00e9dicaments et Technologies pour la Sant\u00e9, 91191 Gif-sur-Yvette, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maxime","family":"Laroche","sequence":"additional","affiliation":[{"name":"Research and Development Department, NG Biotech, Guipry, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Laurent","family":"Dortet","sequence":"additional","affiliation":[{"name":"Team ReSIST, INSERM U1184, School of Medicine, Universit\u00e9 Paris-Saclay, LabEx LERMIT, Le Kremlin-Bic\u00eatre, France"},{"name":"Bacteriology-Hygiene Unit, Assistance Publique\/H\u00f4pitaux de Paris, Bic\u00eatre Hospital, Le Kremlin-Bic\u00eatre, France"},{"name":"French National Reference Centre for Antibiotic Resistance: 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