{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,30]],"date-time":"2026-08-30T01:39:45Z","timestamp":1788053985260,"version":"build-2784847793"},"reference-count":20,"publisher":"Oxford University Press (OUP)","issue":"2","license":[{"start":{"date-parts":[[2020,11,11]],"date-time":"2020-11-11T00:00:00Z","timestamp":1605052800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"funder":[{"name":"Major National R&D Projects of the National Health Department","award":["2018ZX10101003"],"award-info":[{"award-number":["2018ZX10101003"]}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["31770161"],"award-info":[{"award-number":["31770161"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003399","name":"Shanghai Science and Technology Committee","doi-asserted-by":"publisher","award":["17DZ2272900"],"award-info":[{"award-number":["17DZ2272900"]}],"id":[{"id":"10.13039\/501100003399","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003399","name":"Shanghai Science and Technology Committee","doi-asserted-by":"publisher","award":["14495800500"],"award-info":[{"award-number":["14495800500"]}],"id":[{"id":"10.13039\/501100003399","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Shanghai Municipal Commission of Health and Family Planning","award":["2017ZZ01024-001"],"award-info":[{"award-number":["2017ZZ01024-001"]}]},{"name":"Shanghai Sailing Program","award":["19YF1448000"],"award-info":[{"award-number":["19YF1448000"]}]},{"DOI":"10.13039\/501100019069","name":"Chinese Academy of Engineering","doi-asserted-by":"crossref","award":["2019-XY-33"],"award-info":[{"award-number":["2019-XY-33"]}],"id":[{"id":"10.13039\/501100019069","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2021,1,19]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:sec>\n                  <jats:title>Background<\/jats:title>\n                  <jats:p>Echinocandin resistance rarely occurs in clinical Candida parapsilosis isolates and the underlying mechanism is unknown.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Objectives<\/jats:title>\n                  <jats:p>To determine the prevalence of echinocandin resistance and the underlying mechanism for a large collection of C. parapsilosis blood isolates and to determine whether the echinocandin-resistant isolates were clonally related.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Methods<\/jats:title>\n                  <jats:p>C. parapsilosis blood isolates (n\u2009=\u2009213) were subjected to antifungal susceptibility testing (CLSI M27), for micafungin, anidulafungin, amphotericin B and, if appropriate, caspofungin. Hotspot (HS) 1 and HS2 of FKS1 were sequenced for all isolates (n\u2009=\u2009213) and microsatellite typing was performed for echinocandin-resistant isolates.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Results<\/jats:title>\n                  <jats:p>All isolates were susceptible to amphotericin B and two isolates were intermediate to anidulafungin (MIC\u2009=\u20094\u2009mg\/L), while micafungin resistance was noted in four isolates (MIC &amp;gt;8\u2009mg\/L); three of which were also fluconazole resistant and therefore were MDR. Interestingly, micafungin-resistant isolates, but not those intermediate to anidulafungin, carried novel mutation R658G in HS1 of Fks1p; three of which also harboured Y132F+K143R in Erg11. The first isolate (MICR1) was recovered in November 2017 from a patient admitted to paediatric gastroenterology who showed therapeutic failure under caspofungin treatment. MICR2\u2013MICR4 were collected during 2018\u201319 and were recovered from three echinocandin-naive paediatric-surgery patients; the isolates shared the same genotype.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Conclusions<\/jats:title>\n                  <jats:p>Herein, for the first time (to the best of our knowledge), we identified micafungin-resistant C. parapsilosis blood isolates harbouring a novel mutation in HS1 of FKS1, which was likely attributable to in vitro micafungin resistance and in vivo caspofungin therapeutic failure. The acquisition of micafungin-resistant C. parapsilosis isolates in echinocandin-naive patients likely implicates clonal expansion, as supported by the close genetic relatedness of MICR2\u2013MICR4.<\/jats:p>\n               <\/jats:sec>","DOI":"10.1093\/jac\/dkaa419","type":"journal-article","created":{"date-parts":[[2020,11,8]],"date-time":"2020-11-08T20:07:39Z","timestamp":1604866059000},"page":"418-422","source":"Crossref","is-referenced-by-count":50,"title":["Genetically related micafungin-resistant <i>Candida parapsilosis<\/i> blood isolates harbouring novel mutation R658G in hotspot 1 of Fks1p: a new challenge?"],"prefix":"10.1093","volume":"76","author":[{"given":"Amir","family":"Arastehfar","sequence":"first","affiliation":[{"name":"Center for Discovery and Innovation, Hackensack Meridian Health, Nutley, NJ 07110, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Farnaz","family":"Daneshnia","sequence":"additional","affiliation":[{"name":"Westerdijk Fungal Biodiversity Institute, Utrecht, The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S\u00fcleyha","family":"Hilmioglu-Polat","sequence":"additional","affiliation":[{"name":"Division of Mycology, University of Ege, Izmir, Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1174-4182","authenticated-orcid":false,"given":"Macit","family":"Ilkit","sequence":"additional","affiliation":[{"name":"Divison of Mycology, University of \u00c7ukurova, Adana, Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8913-2314","authenticated-orcid":false,"given":"Melike","family":"Yasar","sequence":"additional","affiliation":[{"name":"Division of Mycology, University of Ege, Izmir, Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Furkan","family":"Polat","sequence":"additional","affiliation":[{"name":"Division of Mycology, University of Ege, Izmir, Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dilek Ye\u015fim","family":"Metin","sequence":"additional","affiliation":[{"name":"Division of Mycology, University of Ege, Izmir, Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"\u00dclk\u00fcm Zafer","family":"Dokumcu","sequence":"additional","affiliation":[{"name":"Department of Paediatric Surgery, Faculty of Medicine, University of Ege, Izmir, Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Weihua","family":"Pan","sequence":"additional","affiliation":[{"name":"Shanghai Key Laboratory Molecular Medical Mycology, Shanghai, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ferry","family":"Hagen","sequence":"additional","affiliation":[{"name":"Westerdijk Fungal Biodiversity Institute, Utrecht, The Netherlands"},{"name":"University Medical Center Utrecht, Utrecht, The 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