{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,13]],"date-time":"2026-10-13T15:36:26Z","timestamp":1791905786153,"version":"4.3.4"},"reference-count":25,"publisher":"Oxford University Press (OUP)","issue":"3","license":[{"start":{"date-parts":[[2020,12,1]],"date-time":"2020-12-01T00:00:00Z","timestamp":1606780800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"funder":[{"DOI":"10.13039\/501100004737","name":"National Health Research Institutes","doi-asserted-by":"publisher","award":["IV-108-PP-09"],"award-info":[{"award-number":["IV-108-PP-09"]}],"id":[{"id":"10.13039\/501100004737","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004737","name":"National Health Research Institutes","doi-asserted-by":"publisher","award":["IV-107-PP-09"],"award-info":[{"award-number":["IV-107-PP-09"]}],"id":[{"id":"10.13039\/501100004737","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004737","name":"National Health Research Institutes","doi-asserted-by":"publisher","award":["IV-107-SP-01"],"award-info":[{"award-number":["IV-107-SP-01"]}],"id":[{"id":"10.13039\/501100004737","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100007225","name":"Ministry of Science and Technology","doi-asserted-by":"publisher","award":["107-2320-B-400-010-MY3"],"award-info":[{"award-number":["107-2320-B-400-010-MY3"]}],"id":[{"id":"10.13039\/100007225","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100007225","name":"Ministry of Science and Technology","doi-asserted-by":"publisher","award":["109-2321-B-415-004"],"award-info":[{"award-number":["109-2321-B-415-004"]}],"id":[{"id":"10.13039\/100007225","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"NIH","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2021,2,11]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:sec>\n                  <jats:title>Objectives<\/jats:title>\n                  <jats:p>We aimed to determine susceptibilities of Elizabethkingia spp. to 25 commonly tested and 8 novel antibiotics, and to compare the performance of different susceptibility testing methods.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Methods<\/jats:title>\n                  <jats:p>Clinical isolates of Elizabethkingia spp., Chryseobacterium spp. and Flavobacterium spp. collected during 2002\u201318 (n\u200a=\u200a210) in a nationwide surveillance programme in Taiwan were speciated by 16S rRNA sequencing. MICs were determined by broth microdilution. The broth microdilution results of 18 common antibiotics were compared with those obtained by the VITEK 2 automated system.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Results<\/jats:title>\n                  <jats:p>Among the Elizabethkingia spp. identified (n\u200a=\u200a108), Elizabethkingia anophelis was the most prevalent (n\u200a=\u200a90), followed by Elizabethkingia meningoseptica (n\u200a=\u200a7) and Elizabethkingia miricola cluster [E. miricola (n\u200a=\u200a6), Elizabethkingia bruuniana (n\u200a=\u200a3) and Elizabethkingia ursingii (n\u200a=\u200a2)]. Most isolates were recovered from respiratory or blood specimens from hospitalized, elderly patients. PFGE showed two major and several minor E. anophelis clones. All isolates were resistant to nearly all the tested \u03b2-lactams. Doxycycline, minocycline and trimethoprim\/sulfamethoxazole inhibited &amp;gt;90% of Elizabethkingia spp. Rifampin inhibited E. meningoseptica (100%) and E. anophelis (81.1%). Fluoroquinolones and tigecycline were active against E. meningoseptica and E. miricola cluster isolates. Novel antibiotics, including imipenem\/relebactam, meropenem\/vaborbactam, ceftazidime\/avibactam, cefepime\/zidebactam, delafloxacin, eravacycline and omadacycline were ineffective but lascufloxacin inhibited half of Elizabethkingia spp. The very major discrepancy rates of VITEK 2 were &amp;gt;1.5% for ciprofloxacin, moxifloxacin and vancomycin. Major discrepancy rates were &amp;gt;3% for amikacin, tigecycline, piperacillin\/tazobactam and trimethoprim\/sulfamethoxazole.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Conclusions<\/jats:title>\n                  <jats:p>MDR, absence of standard interpretation criteria and poor intermethod concordance necessitate working guidelines to facilitate future research of emerging Elizabethkingia spp.<\/jats:p>\n               <\/jats:sec>","DOI":"10.1093\/jac\/dkaa499","type":"journal-article","created":{"date-parts":[[2020,11,11]],"date-time":"2020-11-11T20:11:29Z","timestamp":1605125489000},"page":"653-658","source":"Crossref","is-referenced-by-count":35,"title":["Susceptibility of <i>Elizabethkingia<\/i> spp. to commonly tested and novel antibiotics and concordance between broth microdilution and automated testing methods"],"prefix":"10.1093","volume":"76","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6940-6450","authenticated-orcid":false,"given":"Shu-Chen","family":"Kuo","sequence":"first","affiliation":[{"name":"National Institute of Infectious Diseases and Vaccinology, National Health Research Institutes, Miaoli, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mei-Chen","family":"Tan","sequence":"additional","affiliation":[{"name":"National Institute of Infectious Diseases and Vaccinology, National Health Research Institutes, Miaoli, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wei-Cheng","family":"Huang","sequence":"additional","affiliation":[{"name":"National Institute of Infectious Diseases and Vaccinology, National Health Research Institutes, Miaoli, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Han-Chieh","family":"Wu","sequence":"additional","affiliation":[{"name":"National Institute of Infectious Diseases and Vaccinology, National Health Research Institutes, Miaoli, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Feng-Jui","family":"Chen","sequence":"additional","affiliation":[{"name":"National Institute of Infectious Diseases and Vaccinology, National Health Research Institutes, Miaoli, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yu-Chieh","family":"Liao","sequence":"additional","affiliation":[{"name":"Institute of Population Health Sciences, National Health Research Institutes, Miaoli, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hui-Ying","family":"Wang","sequence":"additional","affiliation":[{"name":"National Institute of Infectious Diseases and Vaccinology, National Health Research Institutes, Miaoli, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yih-Ru","family":"Shiau","sequence":"additional","affiliation":[{"name":"National Institute of Infectious Diseases and Vaccinology, National Health Research Institutes, Miaoli, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tsai-Ling","family":"Lauderdale","sequence":"additional","affiliation":[{"name":"National Institute of Infectious Diseases and Vaccinology, National Health Research Institutes, Miaoli, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2020,12,1]]},"reference":[{"key":"2021111806065209300_dkaa499-B1","doi-asserted-by":"crossref","first-page":"55","DOI":"10.1007\/s10482-017-0926-3","article-title":"Revisiting the taxonomy of the genus Elizabethkingia using whole-genome sequencing, optical mapping, and MALDI-TOF, along with proposal of three novel Elizabethkingia species: Elizabethkingia bruuniana sp. nov., Elizabethkingia ursingii sp. nov., and Elizabethkingia occulta sp. nov","volume":"111","author":"Nicholson","year":"2018","journal-title":"Antonie van Leeuwenhoek"},{"key":"2021111806065209300_dkaa499-B2","doi-asserted-by":"crossref","first-page":"1287","DOI":"10.1099\/ijs.0.63541-0","article-title":"Transfer of Chryseobacterium meningosepticum and Chryseobacterium miricola to Elizabethkingia gen. nov. as Elizabethkingia meningoseptica comb. nov. and Elizabethkingia miricola comb. nov","volume":"55","author":"Kim","year":"2005","journal-title":"Int J Syst Evol Microbiol"},{"key":"2021111806065209300_dkaa499-B3","doi-asserted-by":"crossref","first-page":"1271","DOI":"10.1007\/s10096-011-1223-0","article-title":"Clinical features, antimicrobial susceptibilities, and outcomes of Elizabethkingia meningoseptica (Chryseobacterium meningosepticum) bacteremia at a medical center in Taiwan, 1999-2006","volume":"30","author":"Hsu","year":"2011","journal-title":"Eur J Clin Microbiol Infect 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anophelis","volume":"73","author":"Lin","year":"2018","journal-title":"J Antimicrob Chemother"},{"key":"2021111806065209300_dkaa499-B10","doi-asserted-by":"crossref","first-page":"1503","DOI":"10.1093\/jac\/dkz045","article-title":"Fluoroquinolone resistance in carbapenem-resistant Elizabethkingia anophelis: phenotypic and genotypic characteristics of clinical isolates with topoisomerase mutations and comparative genomic analysis","volume":"74","author":"Jian","year":"2019","journal-title":"J Antimicrob Chemother"},{"key":"2021111806065209300_dkaa499-B11","doi-asserted-by":"crossref","first-page":"340","DOI":"10.1016\/j.cmi.2018.04.015","article-title":"Multicentre study evaluating matrix-assisted laser desorption ionization-time of flight mass spectrometry for identification of clinically isolated Elizabethkingia species and analysis of antimicrobial susceptibility","volume":"25","author":"Cheng","year":"2019","journal-title":"Clin Microbiol Infect"},{"key":"2021111806065209300_dkaa499-B12","doi-asserted-by":"crossref","first-page":"15483.","DOI":"10.1038\/ncomms15483","article-title":"Evolutionary dynamics and genomic features of the Elizabethkingia anophelis 2015 to 2016 Wisconsin outbreak strain","volume":"8","author":"Perrin","year":"2017","journal-title":"Nat Commun"},{"key":"2021111806065209300_dkaa499-B13","doi-asserted-by":"crossref","first-page":"855","DOI":"10.1016\/S0140-6736(13)61858-9","article-title":"First case of E. anophelis outbreak in an intensive-care unit","volume":"382","author":"Teo","year":"2013","journal-title":"Lancet"},{"key":"2021111806065209300_dkaa499-B14","doi-asserted-by":"crossref","first-page":"ofx251.","DOI":"10.1093\/ofid\/ofx251","article-title":"Elizabethkingia anophelis: clinical experience of an academic health system in southeastern Wisconsin","volume":"4","author":"Figueroa Castro","year":"2017","journal-title":"Open Forum Infect 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Elizabethkingia species and differentiation between Elizabethkingia anophelis and E. meningoseptica","volume":"57","author":"Kelly","year":"2019","journal-title":"J Clin Microbiol"},{"key":"2021111806065209300_dkaa499-B21","doi-asserted-by":"crossref","first-page":"3097","DOI":"10.1007\/s11274-012-1119-x","article-title":"Occurrence of antimicrobial resistance genes sul and dfrA12 in hospital environmental isolates of Elizabethkingia meningoseptica","volume":"28","author":"Jiang","year":"2012","journal-title":"World J Microbiol Biotechnol"},{"key":"2021111806065209300_dkaa499-B22","volume-title":"Methods for Dilution Antimicrobial Susceptibility Tests for Bacteria That Grow Aerobically\u2014Eleventh Edition: M07","year":"2018"},{"key":"2021111806065209300_dkaa499-B23","doi-asserted-by":"crossref","first-page":"109","DOI":"10.1016\/j.diagmicrobio.2017.10.014","article-title":"rpoB gene sequencing highlights the prevalence of an E. miricola cluster over other Elizabethkingia species among UK cystic fibrosis patients","volume":"90","author":"Kenna","year":"2018","journal-title":"Diagn Microbiol Infect Dis"},{"key":"2021111806065209300_dkaa499-B24","doi-asserted-by":"crossref","first-page":"295.","DOI":"10.3390\/microorganisms7090295","article-title":"Elizabethkingia infections in humans: from genomics to clinics","volume":"7","author":"Lin","year":"2019","journal-title":"Microorganisms"},{"key":"2021111806065209300_dkaa499-B25","doi-asserted-by":"crossref","first-page":"507","DOI":"10.1016\/j.ijantimicag.2017.06.021","article-title":"Role of vancomycin in the treatment of bacteraemia and meningitis caused by Elizabethkingia meningoseptica","volume":"50","author":"Jean","year":"2017","journal-title":"Int J Antimicrob Agents"}],"container-title":["Journal of Antimicrobial 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