{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,8]],"date-time":"2026-09-08T06:36:01Z","timestamp":1788849361756,"version":"build-2803163510"},"reference-count":42,"publisher":"Oxford University Press (OUP)","issue":"8","license":[{"start":{"date-parts":[[2021,5,22]],"date-time":"2021-05-22T00:00:00Z","timestamp":1621641600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"funder":[{"DOI":"10.13039\/501100001713","name":"European and Developing Countries Clinical Trials Partnership","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001713","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000780","name":"European Union","doi-asserted-by":"publisher","award":["TMA2017CDF-1892-HypnoBio"],"award-info":[{"award-number":["TMA2017CDF-1892-HypnoBio"]}],"id":[{"id":"10.13039\/501100000780","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004167","name":"Medicines for Malaria Venture","doi-asserted-by":"publisher","award":["RD-18\u20130067"],"award-info":[{"award-number":["RD-18\u20130067"]}],"id":[{"id":"10.13039\/501100004167","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2021,7,15]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Objectives<\/jats:title>\n                    <jats:p>To evaluate Plasmodium malariae susceptibility to current and lead candidate antimalarial drugs.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>We conducted cross-sectional screening and detection of all Plasmodium species malaria cases, which were nested within a longitudinal prospective study, and an ex vivo assessment of efficacy of a panel of antimalarials against P. malariae and Plasmodium falciparum, both PCR-confirmed mono-infections. Reference compounds tested included chloroquine, lumefantrine, artemether and piperaquine, while candidate antimalarials included the imidazolopiperazine GNF179, a close analogue of KAF156, and the Plasmodium phosphatidylinositol-4-OH kinase (PI4K)-specific inhibitor KDU691.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>We report a high frequency (3%\u201315%) of P. malariae infections with a significant reduction in ex vivo susceptibility to chloroquine, lumefantrine and artemether, which are the current frontline drugs against P. malariae infections. Unlike these compounds, potent inhibition of P. malariae and P. falciparum was observed with piperaquine exposure. Furthermore, we evaluated advanced lead antimalarial compounds. In this regard, we identified strong inhibition of P. malariae using GNF179, a close analogue of KAF156 imidazolopiperazines, which is a novel class of antimalarial drug currently in clinical Phase IIb testing. Finally, in addition to GNF179, we demonstrated that the Plasmodium PI4K-specific inhibitor KDU691 is highly inhibitory against P. malariae and P. falciparum.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>Our data indicated that chloroquine, lumefantrine and artemether may not be suitable for the treatment of P. malariae infections and the potential of piperaquine, as well as new antimalarials imidazolopiperazines and PI4K-specific inhibitor, for P. malariae cure.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1093\/jac\/dkab133","type":"journal-article","created":{"date-parts":[[2021,4,9]],"date-time":"2021-04-09T07:25:17Z","timestamp":1617953117000},"page":"2079-2087","source":"Crossref","is-referenced-by-count":21,"title":["<i>Plasmodium malariae<\/i>\n                    and\n                    <i>Plasmodium falciparum<\/i>\n                    comparative susceptibility to antimalarial drugs in Mali"],"prefix":"10.1093","volume":"76","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9087-8439","authenticated-orcid":false,"given":"Laurent","family":"Dembele","sequence":"first","affiliation":[{"name":"Malaria Research and Training Centre (MRTC), Faculty of Pharmacy, Universit\u00e9 des Sciences, des Techniques et des Technologies de Bamako (USTTB); Point G, P.O. 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Box: 1805, Bamako, Mali"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fanta","family":"Sogore","sequence":"additional","affiliation":[{"name":"Malaria Research and Training Centre (MRTC), Faculty of Pharmacy, Universit\u00e9 des Sciences, des Techniques et des Technologies de Bamako (USTTB); Point G, P.O. Box: 1805, Bamako, Mali"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cheick Papa Oumar","family":"Sangare","sequence":"additional","affiliation":[{"name":"Malaria Research and Training Centre (MRTC), Faculty of Pharmacy, Universit\u00e9 des Sciences, des Techniques et des Technologies de Bamako (USTTB); Point G, P.O. Box: 1805, Bamako, Mali"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aboubecrin Sedhigh","family":"Haidara","sequence":"additional","affiliation":[{"name":"Malaria Research and Training Centre (MRTC), Faculty of Pharmacy, Universit\u00e9 des Sciences, des Techniques et des Technologies de Bamako (USTTB); Point G, P.O. Box: 1805, Bamako, Mali"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aliou","family":"Traore","sequence":"additional","affiliation":[{"name":"Malaria Research and Training Centre (MRTC), Faculty of Pharmacy, Universit\u00e9 des Sciences, des Techniques et des Technologies de Bamako (USTTB); Point G, P.O. Box: 1805, Bamako, Mali"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Seidina A S","family":"Diakit\u00e9","sequence":"additional","affiliation":[{"name":"Malaria Research and Training Centre (MRTC), Faculty of Pharmacy, Universit\u00e9 des Sciences, des Techniques et des Technologies de Bamako (USTTB); Point G, P.O. Box: 1805, Bamako, Mali"},{"name":"West African Centre for Cell Biology of Infectious Pathogens (WACCBIP), Volta Road, Legon, Accra, Ghana"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mahamadou","family":"Diakite","sequence":"additional","affiliation":[{"name":"Malaria Research and Training Centre (MRTC), Faculty of Pharmacy, Universit\u00e9 des Sciences, des Techniques et des Technologies de Bamako (USTTB); Point G, P.O. Box: 1805, Bamako, Mali"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Brice","family":"Campo","sequence":"additional","affiliation":[{"name":"Medicines for Malaria Venture (MMV) ICC Building Entrance G, 3rd floor Route de Pr\u00e9-Bois 20 Post Box 1826 CH-1215, Geneva 15, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gordon A","family":"Awandare","sequence":"additional","affiliation":[{"name":"West African Centre for Cell Biology of Infectious Pathogens (WACCBIP), Volta Road, Legon, Accra, Ghana"},{"name":"Department of Biochemistry, Cell and Molecular Biology, College of Basic and Applied Sciences, University of Ghana, Legon, Ghana"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Abdoulaye A","family":"Djimde","sequence":"additional","affiliation":[{"name":"Malaria Research and Training Centre (MRTC), Faculty of Pharmacy, Universit\u00e9 des Sciences, des Techniques et des Technologies de Bamako (USTTB); Point G, P.O. 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