{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T08:30:30Z","timestamp":1790670630578,"version":"4.1.0"},"reference-count":19,"publisher":"Oxford University Press (OUP)","issue":"Supplement_3","license":[{"start":{"date-parts":[[2021,9,23]],"date-time":"2021-09-23T00:00:00Z","timestamp":1632355200000},"content-version":"vor","delay-in-days":22,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"funder":[{"name":"QIAGEN and Mobidiag"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2021,9,23]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Implementing multiplex PCR or syndromic panel-based testing platforms to detect microbial species that cause acute diarrhoea may guide patient management more effectively and efficiently.<\/jats:p><\/jats:sec><jats:sec><jats:title>Objectives<\/jats:title><jats:p>To assess and compare the performance of two syndromic panel-based testing systems, QIAstat-Dx\u00ae Gastrointestinal Panel V2 (QGI) and the Novodiag\u00ae Bacterial GE+ V2-0 (NGE).<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>The QGI and NGE panels include 16 and 14 bacterial gastrointestinal pathogens, respectively. The performance of the panels was tested retrospectively using 141 positive clinical stool specimens, External Quality Assessment (EQA) panels and spiked faecal specimens.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>For Campylobacter jejuni and coli (n\u200a=\u200a20), Salmonella (n\u200a=\u200a24), Shigella (n\u200a=\u200a13), Yersinia enterocolitica (non-1A biotypes) (n\u200a=\u200a8), Clostridioides difficile (n\u200a=\u200a24) and Vibrio parahaemolyticus (n\u200a=\u200a2), QGI correctly verified 19\/20, 20\/24, 13\/13, 8\/8, 23\/24 and 2\/2, whereas NGE correctly verified 20\/20, 17\/24, 13\/13, 8\/8, 14\/24 and 1\/2. Among diarrhoeagenic Escherichia coli (n\u200a=\u200a29), QGI reported one Shiga toxin-producing E. coli (STEC) stx1a O26:H11 as STEC serotype O157:H7 and NGE failed on one enteropathogenic E. coli, one enteroaggregative E. coli and one STEC (stx2e). Y. enterocolitica biotype 1A (non-pathogenic) (n\u200a=\u200a6) were all positive in QGI, but negative in NGE.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Both QGI and NGE testing panels can improve laboratory workflow and patient management by providing user-friendly platforms that can rapidly detect a number of targets with one specimen. QGI was significantly more sensitive in identifying C. difficile. Both methods had suboptimal detection of Salmonella and this needs to be examined further. The short hands-on time and turnaround time are of value for on-demand testing and use in a high-throughput setting.<\/jats:p><\/jats:sec>","DOI":"10.1093\/jac\/dkab242","type":"journal-article","created":{"date-parts":[[2021,7,30]],"date-time":"2021-07-30T03:23:14Z","timestamp":1627615394000},"page":"iii50-iii57","source":"Crossref","is-referenced-by-count":12,"title":["Verification of analytical bacterial spectrum of QIAstat-Dx\u00ae GI V2 and Novodiag\u00ae Bacterial GE+ V2-0 diagnostic panels"],"prefix":"10.1093","volume":"76","author":[{"given":"J\u00f8rgen","family":"Engberg","sequence":"first","affiliation":[{"name":"Department of Clinical Microbiology, Zealand University Hospital, Koege, Ingemannsvej 46, DK-4200 Slagelse, Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Laus Krems","family":"Vejrum","sequence":"additional","affiliation":[{"name":"Department of Clinical Microbiology, Zealand University Hospital, Koege, Ingemannsvej 46, DK-4200 Slagelse, Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tina Vasehus","family":"Madsen","sequence":"additional","affiliation":[{"name":"Department of Clinical Microbiology, Zealand University Hospital, Koege, Ingemannsvej 46, DK-4200 Slagelse, Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaohui Chen","family":"Nielsen","sequence":"additional","affiliation":[{"name":"Department of Clinical Microbiology, Zealand University Hospital, Koege, Ingemannsvej 46, DK-4200 Slagelse, Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2021,9,23]]},"reference":[{"key":"2021092318111720500_dkab242-B1","doi-asserted-by":"crossref","first-page":"12","DOI":"10.1097\/MCG.0b013e31826df662","article-title":"Acute diarrhea in adults and children: a global perspective","volume":"47","author":"Farthing","year":"2013","journal-title":"J Clin Gastroenterol"},{"key":"2021092318111720500_dkab242-B2","doi-asserted-by":"crossref","first-page":"1545","DOI":"10.1016\/S0140-6736(16)31678-6","article-title":"Global, regional, and national incidence, prevalence, and years lived with disability for 310 diseases and injuries, 1990-2015: a systematic analysis for the Global Burden of Disease Study 2015","volume":"388","year":"2016","journal-title":"Lancet"},{"key":"2021092318111720500_dkab242-B3","doi-asserted-by":"crossref","first-page":"461","DOI":"10.1016\/j.cll.2015.02.006","article-title":"Multiplex polymerase chain reaction tests for detection of pathogens associated with gastroenteritis","volume":"35","author":"Zhang","year":"2015","journal-title":"Clin Lab Med"},{"key":"2021092318111720500_dkab242-B4","doi-asserted-by":"crossref","first-page":"e00024-17","DOI":"10.1128\/CMR.00024-17","article-title":"Syndromic panel-based testing in clinical microbiology","volume":"31","author":"Ramanan","year":"2017","journal-title":"Clin Microbiol Rev"},{"key":"2021092318111720500_dkab242-B5","doi-asserted-by":"crossref","first-page":"O460","DOI":"10.1111\/1469-0691.12476","article-title":"Multiplex molecular testing for management of infectious gastroenteritis in a hospital setting: a comparative diagnostic and clinical utility study","volume":"20","author":"Halligan","year":"2014","journal-title":"Clin Microbiol Infect"},{"key":"2021092318111720500_dkab242-B6","doi-asserted-by":"crossref","first-page":"154","DOI":"10.1016\/j.diagmicrobio.2015.01.007","article-title":"Multiplex gastrointestinal pathogen panels: implications for infection control","volume":"82","author":"Rand","year":"2015","journal-title":"Diagn Microbiol Infect Dis"},{"key":"2021092318111720500_dkab242-B7","doi-asserted-by":"crossref","first-page":"504","DOI":"10.1016\/j.jinf.2014.11.009","article-title":"A cost benefit analysis of the Luminex xTAG Gastrointestinal Pathogen Panel for detection of infectious gastroenteritis in hospitalised patients","volume":"70","author":"Goldenberg","year":"2015","journal-title":"J Infect"},{"key":"2021092318111720500_dkab242-B8","doi-asserted-by":"crossref","first-page":"3667","DOI":"10.1128\/JCM.01637-14","article-title":"Comparative evaluation of two commercial multiplex panels for detection of gastrointestinal pathogens by use of clinical stool specimens","volume":"52","author":"Khare","year":"2014","journal-title":"J Clin Microbiol"},{"key":"2021092318111720500_dkab242-B9","doi-asserted-by":"crossref","first-page":"e0215068","DOI":"10.1371\/journal.pone.0215068","article-title":"Comparative performance evaluation of four commercial multiplex real-time PCR assays for the detection of the diarrhoea-causing protozoa Cryptosporidium hominis\/parvum, Giardia duodenalis and Entamoeba histolytica","volume":"14","author":"Paulos","year":"2019","journal-title":"PLoS One"},{"key":"2021092318111720500_dkab242-B10","doi-asserted-by":"crossref","first-page":"2312","DOI":"10.1128\/JCM.37.7.2312-2316.1999","article-title":"Evaluation of Statens Serum Institut enteric medium for detection of enteric pathogens","volume":"37","author":"Blom","year":"1999","journal-title":"J Clin Microbiol"},{"key":"2021092318111720500_dkab242-B11","doi-asserted-by":"crossref","first-page":"286","DOI":"10.1128\/JCM.38.1.286-291.2000","article-title":"Prevalence of Campylobacter, Arcobacter, Helicobacter, and Sutterella spp. in human fecal samples as estimated by a reevaluation of isolation methods for Campylobacters","volume":"38","author":"Engberg","year":"2000","journal-title":"J Clin Microbiol"},{"key":"2021092318111720500_dkab242-B12","doi-asserted-by":"crossref","first-page":"727","DOI":"10.1007\/s10096-014-2284-7","article-title":"Diagnosis of Clostridium difficile: real-time PCR detection of toxin genes in faecal samples is more sensitive compared to toxigenic culture","volume":"34","author":"Jensen","year":"2015","journal-title":"Eur J Clin Microbiol Infect Dis"},{"key":"2021092318111720500_dkab242-B13","doi-asserted-by":"crossref","first-page":"235","DOI":"10.3390\/genes9050235","article-title":"The most important virulence markers of Yersinia enterocolitica and their role during infection","volume":"9","author":"Bancerz-Kisiel","year":"2018","journal-title":"Genes (Basel)"},{"key":"2021092318111720500_dkab242-B14","year":"2018"},{"key":"2021092318111720500_dkab242-B15","doi-asserted-by":"crossref","first-page":"e01737-19","DOI":"10.1128\/JCM.01737-19","article-title":"Performance of the QIAstat-Dx gastrointestinal panel for diagnosing infectious gastroenteritis","volume":"58","author":"Boers","year":"2020","journal-title":"J Clin Microbiol"},{"key":"2021092318111720500_dkab242-B16","first-page":"2049936120959561","article-title":"Evaluating appropriateness and diagnostic stewardship opportunities of multiplex polymerase chain reaction gastrointestinal testing within a hospital system","volume":"7","author":"O'Neal","year":"2020","journal-title":"Ther Adv Infect"},{"key":"2021092318111720500_dkab242-B17","doi-asserted-by":"crossref","first-page":"e00148-18","DOI":"10.1128\/JCM.00148-18","article-title":"Rapid molecular detection of gastrointestinal pathogens and its role in antimicrobial stewardship","volume":"56","author":"Keske","year":"2018","journal-title":"J Clin Microbiol"},{"key":"2021092318111720500_dkab242-B18","doi-asserted-by":"crossref","first-page":"941","DOI":"10.1128\/JCM.01489-10","article-title":"New methods for detection of campylobacters in stool samples in comparison to culture","volume":"49","author":"Bess\u00e8de","year":"2011","journal-title":"J Clin Microbiol"},{"key":"2021092318111720500_dkab242-B19","doi-asserted-by":"crossref","first-page":"2103","DOI":"10.1007\/s10096-019-03646-4","article-title":"Multicenter evaluation of the new QIAstat Gastrointestinal Panel for the rapid syndromic testing of acute gastroenteritis","volume":"38","author":"Hannet","year":"2019","journal-title":"Eur J Clin Microbiol Infect Dis"}],"container-title":["Journal of Antimicrobial Chemotherapy"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/academic.oup.com\/jac\/article-pdf\/76\/Supplement_3\/iii50\/40433696\/dkab242.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"http:\/\/academic.oup.com\/jac\/article-pdf\/76\/Supplement_3\/iii50\/40433696\/dkab242.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,1,5]],"date-time":"2023-01-05T22:27:00Z","timestamp":1672957620000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/jac\/article\/76\/Supplement_3\/iii50\/6374613"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,9,1]]},"references-count":19,"journal-issue":{"issue":"Supplement_3","published-online":{"date-parts":[[2021,9,23]]},"published-print":{"date-parts":[[2021,9,23]]}},"URL":"https:\/\/doi.org\/10.1093\/jac\/dkab242","relation":{},"ISSN":["0305-7453","1460-2091"],"issn-type":[{"value":"0305-7453","type":"print"},{"value":"1460-2091","type":"electronic"}],"subject":[],"published-other":{"date-parts":[[2021,9,1]]},"published":{"date-parts":[[2021,9,1]]}}}