{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,11,4]],"date-time":"2026-11-04T09:38:27Z","timestamp":1793785107976,"version":"4.6.1"},"reference-count":92,"publisher":"Oxford University Press (OUP)","issue":"12","license":[{"start":{"date-parts":[[2021,8,30]],"date-time":"2021-08-30T00:00:00Z","timestamp":1630281600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2021,11,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Staphylococcus aureus \u2013 a major aetiological agent of bone and joint infection (BJI) \u2013 is associated with a high risk of relapse and chronicity, in part due to its ability to invade and persist in non-professional phagocytic bone cells such as osteoblasts. This intracellular reservoir protects S. aureus from the action of the immune system and most antibiotics. To date, the choice of antimicrobial strategies for BJI treatment mostly relies on standard susceptibility testing, bone penetration of antibiotics and their \u2018antibiofilm\u2019 activity. Despite the role of intracellular persistent S. aureus in the development of chronic infection, the ability of antibiotics to target the S. aureus intraosteoblastic reservoir is not considered in therapeutic choices but might represent a key determinant of treatment outcome. This review provides an overview of the intracellular pharmacokinetics of antistaphylococcal drugs used in the treatment of BJI and of their ability to target intraosteoblastic S. aureus. Thirteen studies focusing on the intraosteoblastic activity of antibiotics against S. aureus were reviewed, all relying on in vitro models of osteoblast infection. Despite varying incubation times, multiplicities of infection, bacterial strains, and the types of infected cell lines, rifamycins and fluoroquinolones remain the two most potent antimicrobial classes for intraosteoblastic S. aureus eradication, consistent with clinical data showing a superiority of this combination therapy in S. aureus orthopaedic device-related infections.<\/jats:p>","DOI":"10.1093\/jac\/dkab301","type":"journal-article","created":{"date-parts":[[2021,8,16]],"date-time":"2021-08-16T11:12:05Z","timestamp":1629112325000},"page":"3091-3102","source":"Crossref","is-referenced-by-count":26,"title":["<i>In vitro<\/i>antibiotic activity against intraosteoblastic<i>Staphylococcus aureus<\/i>: a narrative review of the literature"],"prefix":"10.1093","volume":"76","author":[{"given":"Florian C","family":"Marro","sequence":"first","affiliation":[{"name":"CIRI\u2014Centre International de Recherche en Infectiologie, Inserm, U1111, Universit\u00e9 Claude Bernard Lyon 1, CNRS, UMR5308, Ecole Normale Sup\u00e9rieure de Lyon, Univ Lyon, F-69007 Lyon, France"},{"name":"Evotec ID Lyon, In Vitro Biology, Infectious Diseases and Antibacterials Unit, Gerland, 69007 Lyon, France"},{"name":"Universit\u00e9 Claude Bernard Lyon 1, Lyon, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"L\u00e9lia","family":"Abad","sequence":"additional","affiliation":[{"name":"CIRI\u2014Centre International de Recherche en Infectiologie, Inserm, U1111, Universit\u00e9 Claude Bernard Lyon 1, CNRS, UMR5308, Ecole Normale Sup\u00e9rieure de Lyon, Univ Lyon, F-69007 Lyon, France"},{"name":"Universit\u00e9 Claude Bernard Lyon 1, Lyon, France"},{"name":"Laboratoire de bact\u00e9riologie, Institut des Agents Infectieux, French National Reference Center for Staphylococci, Hospices Civils de Lyon, Lyon, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ariel J","family":"Blocker","sequence":"additional","affiliation":[{"name":"Evotec ID Lyon, In Vitro Biology, Infectious Diseases and Antibacterials Unit, Gerland, 69007 Lyon, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fr\u00e9d\u00e9ric","family":"Laurent","sequence":"additional","affiliation":[{"name":"CIRI\u2014Centre International de Recherche en Infectiologie, Inserm, U1111, Universit\u00e9 Claude Bernard Lyon 1, CNRS, UMR5308, Ecole Normale Sup\u00e9rieure de Lyon, Univ Lyon, F-69007 Lyon, France"},{"name":"Universit\u00e9 Claude Bernard Lyon 1, Lyon, France"},{"name":"Laboratoire de bact\u00e9riologie, Institut des Agents Infectieux, French National Reference Center for Staphylococci, Hospices Civils de Lyon, Lyon, France"},{"name":"Centre de R\u00e9f\u00e9rence pour la prise en charge des Infections ost\u00e9o-articulaires complexes (CRIOAc) Lyon, Hospices Civils de Lyon, Lyon, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J\u00e9r\u00f4me","family":"Josse","sequence":"additional","affiliation":[{"name":"CIRI\u2014Centre International de Recherche en Infectiologie, Inserm, U1111, Universit\u00e9 Claude Bernard Lyon 1, CNRS, UMR5308, Ecole Normale Sup\u00e9rieure de Lyon, Univ Lyon, F-69007 Lyon, France"},{"name":"Universit\u00e9 Claude Bernard Lyon 1, Lyon, France"},{"name":"Centre de R\u00e9f\u00e9rence pour la prise en charge des Infections ost\u00e9o-articulaires complexes (CRIOAc) Lyon, Hospices Civils de Lyon, Lyon, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Florent","family":"Valour","sequence":"additional","affiliation":[{"name":"CIRI\u2014Centre International de Recherche en Infectiologie, Inserm, U1111, Universit\u00e9 Claude Bernard Lyon 1, CNRS, UMR5308, Ecole Normale Sup\u00e9rieure de Lyon, Univ Lyon, F-69007 Lyon, France"},{"name":"Universit\u00e9 Claude Bernard Lyon 1, Lyon, France"},{"name":"Centre de R\u00e9f\u00e9rence pour la prise en charge des Infections ost\u00e9o-articulaires complexes (CRIOAc) Lyon, Hospices Civils de Lyon, Lyon, 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