{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,25]],"date-time":"2026-06-25T11:08:52Z","timestamp":1782385732662,"version":"3.54.5"},"reference-count":55,"publisher":"Oxford University Press (OUP)","issue":"3","license":[{"start":{"date-parts":[[2021,12,11]],"date-time":"2021-12-11T00:00:00Z","timestamp":1639180800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"funder":[{"name":"Overseas Famous Teacher Project of Guangdong Provincial Department of Science and Technology","award":["2020A1414010136"],"award-info":[{"award-number":["2020A1414010136"]}]},{"name":"Medical Science and Technology Research Foundation of Guangdong Province","award":["A2019010"],"award-info":[{"award-number":["A2019010"]}]},{"name":"Medical Science and Technology Research Foundation of Guangdong Province","award":["A2021139"],"award-info":[{"award-number":["A2021139"]}]},{"name":"Guangdong Traditional Chinese Medicine Research Project","award":["20191230"],"award-info":[{"award-number":["20191230"]}]},{"name":"Guangdong Traditional Chinese Medicine Research Project","award":["20211277"],"award-info":[{"award-number":["20211277"]}]},{"name":"Guangdong Provincial Medical Research Fund","award":["B2020149"],"award-info":[{"award-number":["B2020149"]}]},{"name":"Scientific Research Initiative Project of Southern Medical University"},{"name":"Project of Youth Science and Technology Personnel Training","award":["PY2018N100"],"award-info":[{"award-number":["PY2018N100"]}]},{"name":"Guangdong Province","award":["2018KQNCX025"],"award-info":[{"award-number":["2018KQNCX025"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2022,2,23]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Gonorrhoea, caused by Neisseria gonorrhoeae, has spread worldwide. Strains resistant to most antibiotics, including ceftriaxone and azithromycin, have emerged to an alarming level. Rapid testing for N. gonorrhoeae and its antimicrobial resistance will therefore contribute to clinical decision making for early diagnosis and rational drug use.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>A Cas13a-based assay (specific high-sensitivity enzymatic reporter unlocking; SHERLOCK) was developed for N. gonorrhoeae detection (porA gene) and azithromycin resistance identification (A2059G, C2611T). Assays were evaluated for sensitivity with purified dsDNA and specificity with 17 non-gonococcal strains. Performance of SHERLOCK (porA) was compared with Roche Cobas 4800 using 43 urine samples. Identification of azithromycin resistance mutations (A2059G, C2611T) was evaluated using a total of 84 clinical isolates and 18 urine samples. Lateral flow was tested for this assay as a readout tool. Moreover, we directly assayed 27 urethral swabs from patients with urethritis to evaluate their status in terms of N. gonorrhoeae infection and azithromycin resistance.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>The SHERLOCK assay was successfully developed with a sensitivity of 10 copies\/reaction, except 100 copies\/reaction for A2059G, and no cross-reaction with other species. Comparison of the SHERLOCK assay with the Cobas 4800 revealed 100% concordance within 18 positive and 25 negative urine samples. Of the 84 isolates, 21 strains with azithromycin resistance mutations were distinguished and further verified by sequencing and MIC determination. In addition, 62.96% (17\/27) strains from swab samples were detected with no mutant strains confirmed by sequencing.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>The SHERLOCK assay for rapid N. gonorrhoeae detection combined with azithromycin resistance testing is a promising method for application in clinical practice.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1093\/jac\/dkab447","type":"journal-article","created":{"date-parts":[[2021,12,1]],"date-time":"2021-12-01T15:14:04Z","timestamp":1638371644000},"page":"656-664","source":"Crossref","is-referenced-by-count":20,"title":["Development and application of Cas13a-based diagnostic assay for\n                    <i>Neisseria gonorrhoeae<\/i>\n                    detection and azithromycin resistance identification"],"prefix":"10.1093","volume":"77","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5463-9852","authenticated-orcid":false,"given":"Hao","family":"Luo","sequence":"first","affiliation":[{"name":"Dermatology Hospital, Southern Medical University, Guangzhou 510091, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4062-3865","authenticated-orcid":false,"given":"Wentao","family":"Chen","sequence":"additional","affiliation":[{"name":"Dermatology Hospital, Southern Medical University, Guangzhou 510091, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhida","family":"Mai","sequence":"additional","affiliation":[{"name":"Dermatology Hospital, Southern Medical University, Guangzhou 510091, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jianjiang","family":"Yang","sequence":"additional","affiliation":[{"name":"Dermatology Hospital, Southern Medical University, Guangzhou 510091, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaomian","family":"Lin","sequence":"additional","affiliation":[{"name":"Dermatology Hospital, Southern Medical University, Guangzhou 510091, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lihong","family":"Zeng","sequence":"additional","affiliation":[{"name":"Dermatology Hospital, Southern Medical University, Guangzhou 510091, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuying","family":"Pan","sequence":"additional","affiliation":[{"name":"Dermatology Hospital, Southern Medical University, Guangzhou 510091, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qinghui","family":"Xie","sequence":"additional","affiliation":[{"name":"Guangdong Dermatology Clinical College, Anhui Medical University, Hefei 230022, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qingqing","family":"Xu","sequence":"additional","affiliation":[{"name":"Guangdong Dermatology Clinical College, Anhui Medical University, Hefei 230022, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaoxiao","family":"Li","sequence":"additional","affiliation":[{"name":"Hubei Key Laboratory of Tumor Microenvironment and Immunotherapy, Three Gorges University, Yichang 443002, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yiwen","family":"Liao","sequence":"additional","affiliation":[{"name":"Dermatology Hospital, Southern Medical University, Guangzhou 510091, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhanqin","family":"Feng","sequence":"additional","affiliation":[{"name":"Dermatology Hospital, Southern Medical University, Guangzhou 510091, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiangli","family":"Ou","sequence":"additional","affiliation":[{"name":"Dermatology Hospital, Southern Medical University, Guangzhou 510091, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaolin","family":"Qin","sequence":"additional","affiliation":[{"name":"Dermatology Hospital, Southern Medical University, Guangzhou 510091, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Heping","family":"Zheng","sequence":"additional","affiliation":[{"name":"Dermatology Hospital, Southern Medical University, Guangzhou 510091, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2021,12,11]]},"reference":[{"key":"2022022312584465600_dkab447-B1","doi-asserted-by":"crossref","first-page":"79","DOI":"10.1038\/s41572-019-0128-6","article-title":"Gonorrhoea","volume":"5","author":"Unemo","year":"2019","journal-title":"Nat Rev Dis 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