{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T07:19:39Z","timestamp":1790752779687,"version":"4.1.0"},"reference-count":28,"publisher":"Oxford University Press (OUP)","issue":"8","license":[{"start":{"date-parts":[[2022,6,9]],"date-time":"2022-06-09T00:00:00Z","timestamp":1654732800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2022,7,28]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:sec>\n                  <jats:title>Objectives<\/jats:title>\n                  <jats:p>Implementation of an antibiotic resistance detection tool in Legionella daily surveillance at the French National Reference Centre for Legionella.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Methods<\/jats:title>\n                  <jats:p>Systematic WGS of Legionella pneumophila isolates and bioinformatics detection of specific mutations linked to antibiotic resistance. Phenotypic validation of antibiotic resistance detected by WGS was performed by the broth microdilution method.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Results<\/jats:title>\n                  <jats:p>More than 3000 L. pneumophila strains were screened for antibiotic resistance. A macrolide resistance-associated A2052G mutation in the 23S rRNA gene was identified in the genome of eight isolates from a hotel water network. High-level macrolide resistance (i.e. MICs of 1024\u20132048\u2005mg\/L for azithromycin and erythromycin) with no cross-resistance to other antimicrobials was phenotypically confirmed by antimicrobial susceptibility testing for the eight isolates.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Conclusions<\/jats:title>\n                  <jats:p>Systematic WGS of L. pneumophila is a powerful tool for first-line high-throughput screening of antibiotic resistance before phenotypic validation.<\/jats:p>\n               <\/jats:sec>","DOI":"10.1093\/jac\/dkac173","type":"journal-article","created":{"date-parts":[[2022,6,9]],"date-time":"2022-06-09T10:20:21Z","timestamp":1654770021000},"page":"2167-2170","source":"Crossref","is-referenced-by-count":15,"title":["Detection of highly macrolide-resistant <i>Legionella pneumophila<\/i> strains from a hotel water network using systematic whole-genome sequencing"],"prefix":"10.1093","volume":"77","author":[{"given":"Christophe","family":"Ginevra","sequence":"first","affiliation":[{"name":"Hospices Civils de Lyon, Groupement Hospitalier Nord, Institut des Agents Infectieux, Centre National de R\u00e9f\u00e9rence des L\u00e9gionelles , Lyon , France"},{"name":"Centre International de Recherche en Infectiologie (CIRI), Team Legionella Pathogenesis, Univ Lyon, Inserm, U1111, Universite\u0301 Claude Bernard Lyon 1, CNRS, UMR5308, ENS de Lyon , F-69007, Lyon , France"},{"name":"Hospices Civils de Lyon, Groupement Hospitalier Nord, Institut des Agents Infectieux, Plateforme GenEPII , Lyon , France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Laetitia","family":"Beraud","sequence":"additional","affiliation":[{"name":"Hospices Civils de Lyon, Groupement Hospitalier Nord, Institut des Agents Infectieux, Centre National de R\u00e9f\u00e9rence des L\u00e9gionelles , Lyon , France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Isabelle","family":"Pionnier","sequence":"additional","affiliation":[{"name":"Agence R\u00e9gionale de Sant\u00e9 Auvergne-Rh\u00f4ne-Alpes , Lyon , France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kassandra","family":"Sallabery","sequence":"additional","affiliation":[{"name":"Agence R\u00e9gionale de Sant\u00e9 Auvergne-Rh\u00f4ne-Alpes , Lyon , France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Houcine","family":"Bentayeb","sequence":"additional","affiliation":[{"name":"Centre Hospitalier Saint-Quentin, Service de Pneumologie , Saint-Quentin , France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bruno","family":"Simon","sequence":"additional","affiliation":[{"name":"Hospices Civils de Lyon, Groupement Hospitalier Nord, Institut des Agents Infectieux, Plateforme GenEPII , Lyon , 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Groupement Hospitalier Nord, Institut des Agents Infectieux, Centre National de R\u00e9f\u00e9rence des L\u00e9gionelles , Lyon , France"},{"name":"Centre International de Recherche en Infectiologie (CIRI), Team Legionella Pathogenesis, Univ Lyon, Inserm, U1111, Universite\u0301 Claude Bernard Lyon 1, CNRS, UMR5308, ENS de Lyon , F-69007, Lyon , France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"V\u00e9ronique","family":"Decroix","sequence":"additional","affiliation":[{"name":"Centre Hospitalier Saint-Quentin, Laboratoire de Biologie M\u00e9dicale , Saint-Quentin , France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christine","family":"Campese","sequence":"additional","affiliation":[{"name":"Sant\u00e9 Publique France, Unit\u00e9 des infections respiratoires et vaccinations , Saint-Maurice , France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sophie","family":"Jarraud","sequence":"additional","affiliation":[{"name":"Hospices Civils de Lyon, Groupement Hospitalier Nord, Institut des Agents Infectieux, Centre National de R\u00e9f\u00e9rence des L\u00e9gionelles , Lyon , France"},{"name":"Centre International de Recherche en Infectiologie (CIRI), Team Legionella Pathogenesis, Univ Lyon, Inserm, U1111, Universite\u0301 Claude Bernard Lyon 1, CNRS, UMR5308, ENS de Lyon , F-69007, Lyon , France"},{"name":"Hospices Civils de Lyon, Groupement Hospitalier Nord, Institut des Agents Infectieux, Plateforme GenEPII , Lyon , France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ghislaine","family":"Descours","sequence":"additional","affiliation":[{"name":"Hospices Civils de Lyon, Groupement Hospitalier Nord, Institut des Agents Infectieux, Centre National de R\u00e9f\u00e9rence des L\u00e9gionelles , Lyon , France"},{"name":"Centre International de Recherche en Infectiologie (CIRI), Team Legionella Pathogenesis, Univ Lyon, Inserm, U1111, Universite\u0301 Claude Bernard Lyon 1, CNRS, UMR5308, ENS de 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