{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,29]],"date-time":"2026-03-29T13:44:26Z","timestamp":1774791866923,"version":"3.50.1"},"reference-count":27,"publisher":"Oxford University Press (OUP)","issue":"9","license":[{"start":{"date-parts":[[2022,6,16]],"date-time":"2022-06-16T00:00:00Z","timestamp":1655337600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"funder":[{"name":"Projects of Medical and Health Technology Development Program in Shandong Province","award":["2017WS175"],"award-info":[{"award-number":["2017WS175"]}]},{"name":"Natural Science Foundations of Shandong Province","award":["ZR2016HL44"],"award-info":[{"award-number":["ZR2016HL44"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2022,8,25]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:sec>\n                  <jats:title>Background<\/jats:title>\n                  <jats:p>Only one additional specific SNP in blaTEM-135 in penicillinase-producing Neisseria gonorrhoeae (PPNG) isolates is required for TEM-135 to evolve into an ESBL that is capable of degrading all extended-spectrum cephalosporins (ESCs).<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Methods<\/jats:title>\n                  <jats:p>Identification of the blaTEM-135 gene was achieved by performing a mismatch amplification mutation assay PCR. PPNG isolates were then genotyped using MLST and N. gonorrhoeae multiantigen sequence typing (NG-MAST) techniques. Finally, phylogenetic analyses based on MLST and NG-MAST were performed on all of the PPNG isolates, and genogroups were classified according to the phylogenetic relatedness on the basis of each MLST and NG-MAST gene typing and the blaTEM allele varieties.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Results<\/jats:title>\n                  <jats:p>Among the 197 PPNG isolates, one (0.5%) isolate possessed the blaTEM-220 allele, 90 (45.7%) possessed the blaTEM-135 allele, and 106 (53.8%) possessed the blaTEM-1 allele. Among the blaTEM-135-carrying PPNG isolates, MLST ST8109 (n\u200a=\u200a30) and NG-MAST ST12199 (n\u200a=\u200a5), ST12754 (n\u200a=\u200a5) and ST15073 (n\u200a=\u200a5) were the most prevalent in the Shandong province of China. The phylogenetic analyses revealed that MLST ST8109, ST8140 and ST14417, mainly carrying the blaTEM-135 allele, belonged to the same genogroup, G1, while NG-MAST ST12199 and ST15073, mainly carrying the blaTEM-135 allele, belonged to the same genogroup, Ga.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Conclusions<\/jats:title>\n                  <jats:p>This study has shown that the molecular epidemiology of PPNG isolates carrying the blaTEM-135 allele in Shandong has a dynamically changing status. Therefore, it is very pressing to continuously monitor the prevalence and mutation of the blaTEM-135 allele and the genetic epidemiology of PPNG isolates carrying the blaTEM-135 allele in this district.<\/jats:p>\n               <\/jats:sec>","DOI":"10.1093\/jac\/dkac192","type":"journal-article","created":{"date-parts":[[2022,6,16]],"date-time":"2022-06-16T04:50:41Z","timestamp":1655355041000},"page":"2406-2413","source":"Crossref","is-referenced-by-count":6,"title":["High prevalence of <i>bla<\/i>TEM-135 and genetic epidemiology of <i>bla<\/i>TEM-135-carrying <i>Neisseria gonorrhoeae<\/i> isolates in Shandong, China, 2017\u201319"],"prefix":"10.1093","volume":"77","author":[{"given":"Lihong","family":"Zhao","sequence":"first","affiliation":[{"name":"Department of Clinical Laboratory, Tai\u2019an City Central Hospital , Tai\u2019an 271000 , China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Aihua","family":"Liu","sequence":"additional","affiliation":[{"name":"Central Laboratory, Tai\u2019an City Central Hospital , Tai\u2019an 271000 , China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ruiying","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Gynaecology, Tai\u2019an City Central Hospital , Tai\u2019an 271000 , China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhijun","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Clinical Laboratory, Tai\u2019an City Central Hospital , Tai\u2019an 271000 , China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ying","family":"Jia","sequence":"additional","affiliation":[{"name":"Department of Clinical Laboratory, Tai\u2019an City Central Hospital , Tai\u2019an 271000 , 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