{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,9]],"date-time":"2026-02-09T15:22:42Z","timestamp":1770650562758,"version":"3.49.0"},"reference-count":18,"publisher":"Oxford University Press (OUP)","issue":"9","license":[{"start":{"date-parts":[[2022,6,24]],"date-time":"2022-06-24T00:00:00Z","timestamp":1656028800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"funder":[{"name":"Council of Scientific Research"},{"DOI":"10.13039\/501100001332","name":"CSIR","doi-asserted-by":"crossref","id":[{"id":"10.13039\/501100001332","id-type":"DOI","asserted-by":"crossref"}]},{"name":"Department of Biotechnology","award":["BT\/PR32004\/MED\/29\/1435\/2019"],"award-info":[{"award-number":["BT\/PR32004\/MED\/29\/1435\/2019"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2022,8,25]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:sec>\n                  <jats:title>Background<\/jats:title>\n                  <jats:p>Antimicrobial resistance is a growing menace, claiming millions of lives all over the world. In this context, drug repurposing is one approach gaining interest as a suitable alternative to conventional drug discovery and development.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Methods<\/jats:title>\n                  <jats:p>Whole-cell assays were used to screen FDA-approved drugs to identify novel antimicrobial agents active against bacterial pathogens. Following identification of nitazoxanide, its various characteristics, such as antimicrobial activity against MDR isolates, time\u2013kill kinetics, ability to synergize with approved drugs, antibiofilm activity and ability to generate resistance in Staphylococcus aureus, were determined, followed by determination of its in vivo potential against MDR S. aureus.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Results<\/jats:title>\n                  <jats:p>Nitazoxanide demonstrated a potent in vitro antistaphylococcal profile, including equipotent activity against clinical drug-resistant S. aureus and Enterococcus spp. Nitazoxanide exhibited concentration-dependent killing, significantly eradicated preformed S. aureus biofilm and S. aureus did not generate resistance to it. Nitazoxanide strongly synergized with linezolid both in vitro and in vivo against linezolid-susceptible and -resistant S. aureus, displaying superior activity to untreated control and drug-alone treatment groups.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Conclusions<\/jats:title>\n                  <jats:p>Nitazoxanide can be utilized in combination with linezolid against infections caused by linezolid-resistant S. aureus as it exhibits strong synergism in vitro and in vivo.<\/jats:p>\n               <\/jats:sec>","DOI":"10.1093\/jac\/dkac201","type":"journal-article","created":{"date-parts":[[2022,6,24]],"date-time":"2022-06-24T12:41:33Z","timestamp":1656074493000},"page":"2456-2460","source":"Crossref","is-referenced-by-count":13,"title":["Nitazoxanide potentiates linezolid against linezolid-resistant <i>Staphylococcus aureus in vitro<\/i> and <i>in vivo<\/i>"],"prefix":"10.1093","volume":"77","author":[{"given":"Grace","family":"Kaul","sequence":"first","affiliation":[{"name":"Division of Microbiology, CSIR-Central Drug Research Institute , Sitapur Road, Sector 10, Janakipuram Extension, Lucknow-226031, Uttar Pradesh , India"},{"name":"Academy of Scientific and Innovative Research (AcSIR) , Ghaziabad-201002 , India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Abdul","family":"Akhir","sequence":"additional","affiliation":[{"name":"Division of Microbiology, CSIR-Central Drug Research Institute , Sitapur Road, Sector 10, Janakipuram Extension, Lucknow-226031, Uttar Pradesh , India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9137-5259","authenticated-orcid":false,"given":"Manjulika","family":"Shukla","sequence":"additional","affiliation":[{"name":"Division of Microbiology, CSIR-Central Drug Research Institute , Sitapur Road, Sector 10, Janakipuram Extension, Lucknow-226031, Uttar Pradesh , India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kundan S","family":"Rawat","sequence":"additional","affiliation":[{"name":"Academy of Scientific and Innovative Research (AcSIR) , Ghaziabad-201002 , India"},{"name":"Division of Medicinal and Process Chemistry, CSIR-Central Drug Research Institute , Sitapur Road, Sector 10, Janakipuram Extension, Lucknow-226031, Uttar Pradesh , India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chandra P","family":"Sharma","sequence":"additional","affiliation":[{"name":"Division of Medicinal and Process Chemistry, CSIR-Central Drug Research Institute , Sitapur Road, Sector 10, Janakipuram Extension, Lucknow-226031, Uttar Pradesh , India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Komal G","family":"Sangu","sequence":"additional","affiliation":[{"name":"Academy of Scientific and Innovative Research (AcSIR) , Ghaziabad-201002 , India"},{"name":"Department of Organic Synthesis and Process Chemistry, CSIR-Indian Institute of Chemical Technology , Tarnaka, Hyderabad 500007 , India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Haridas B","family":"Rode","sequence":"additional","affiliation":[{"name":"Academy of Scientific and Innovative Research (AcSIR) , Ghaziabad-201002 , India"},{"name":"Department of Organic Synthesis and Process Chemistry, CSIR-Indian Institute of Chemical Technology , Tarnaka, Hyderabad 500007 , India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Atul","family":"Goel","sequence":"additional","affiliation":[{"name":"Academy of Scientific and Innovative Research (AcSIR) , Ghaziabad-201002 , India"},{"name":"Division of Medicinal and Process Chemistry, CSIR-Central Drug Research Institute , Sitapur Road, Sector 10, Janakipuram Extension, Lucknow-226031, Uttar Pradesh , India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8823-6074","authenticated-orcid":false,"given":"Sidharth","family":"Chopra","sequence":"additional","affiliation":[{"name":"Division of Microbiology, CSIR-Central Drug Research Institute , Sitapur Road, Sector 10, Janakipuram Extension, Lucknow-226031, Uttar Pradesh , India"},{"name":"Academy of Scientific and Innovative Research (AcSIR) , Ghaziabad-201002 , India"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[2022,6,24]]},"reference":[{"key":"2022082518385844800_dkac201-B1","author":"WHO"},{"key":"2022082518385844800_dkac201-B2","author":"WHO"},{"key":"2022082518385844800_dkac201-B3","doi-asserted-by":"crossref","first-page":"430","DOI":"10.1093\/femsre\/fux007","article-title":"Antibiotic resistance in Staphylococcus aureus. 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