{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,1]],"date-time":"2026-03-01T12:21:49Z","timestamp":1772367709998,"version":"3.50.1"},"reference-count":18,"publisher":"Oxford University Press (OUP)","issue":"12","license":[{"start":{"date-parts":[[2022,10,22]],"date-time":"2022-10-22T00:00:00Z","timestamp":1666396800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"funder":[{"DOI":"10.13039\/501100003593","name":"Conselho Nacional de Desenvolvimento Cient\u00edfico e Tecnol\u00f3gico","doi-asserted-by":"crossref","id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100004263","name":"Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa do Estado do Rio Grande do Sul","doi-asserted-by":"crossref","id":[{"id":"10.13039\/501100004263","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100002322","name":"Coordena\u00e7\u00e3o de Aperfei\u00e7oamento de Pessoal de N\u00edvel Superior","doi-asserted-by":"crossref","id":[{"id":"10.13039\/501100002322","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2022,11,28]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:sec>\n                  <jats:title>Objectives<\/jats:title>\n                  <jats:p>The HIV-1 genetic diversity and the presence of transmitted drug resistance mutations (TDRMs) against integrase strand transfer inhibitors (INSTIs) were assessed sequencing samples of antiretroviral (ARV)-naive HIV-1-infected individuals from South Brazil.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Methods<\/jats:title>\n                  <jats:p>Viral RNA from 42 ART-naive individuals was submitted to complete HIV-1 integrase gene amplification by RT\u2013PCR and sequencing.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Results<\/jats:title>\n                  <jats:p>Viral strains carrying TDRMs against INSTIs were not detected in the present study. However, the polymorphisms L74M and L74I were each observed in 4.8% of the individuals. These accessory mutations have been reported as putative causes of TDRMs in ART with raltegravir, but only when associated with additional major mutations. When submitted to HIV-1 subtyping, 50% were classified as subtype C, 21% as recombinant BC, 19% as subtype B, 4.8% as subtype F1 and 4.8% as recombinant CF1.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Conclusions<\/jats:title>\n                  <jats:p>All 42 ARV-naive individuals were apparently susceptible to INSTIs, included in the Brazilian therapeutic guideline since 2009. To the best of our knowledge, this is the first study to evaluate TDRMs against INSTIs in Brazil. The most prevalent HIV-1 subtypes were subtype C, followed by the recombinant BC and subtype B, which is in agreement with previous studies. However, the presence of subtype F1 and recombinant CF1 reported herein was not observed in previous studies.<\/jats:p>\n               <\/jats:sec>","DOI":"10.1093\/jac\/dkac355","type":"journal-article","created":{"date-parts":[[2022,10,22]],"date-time":"2022-10-22T17:08:07Z","timestamp":1666458487000},"page":"3510-3514","source":"Crossref","is-referenced-by-count":8,"title":["HIV-1 genetic diversity and transmitted drug resistance to integrase strand transfer inhibitors among recently diagnosed adults in Porto Alegre, South Brazil"],"prefix":"10.1093","volume":"77","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3547-4033","authenticated-orcid":false,"given":"Cynara Carvalho","family":"Nunes","sequence":"first","affiliation":[{"name":"Instituto de Ci\u00eancias da Sa\u00fade, Laborat\u00f3rio de Microbiologia Molecular, Universidade Feevale , Novo Hamburgo, RS , Brazil"},{"name":"Servi\u00e7o de Atendimento Especializado (SAE) Santa Marta, Secretaria Municipal de Sa\u00fade de 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Mattos","sequence":"additional","affiliation":[{"name":"Servi\u00e7o de Atendimento Especializado (SAE) Santa Marta, Secretaria Municipal de Sa\u00fade de Porto Alegre , Porto Alegre, RS , Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Daila Haemk","family":"Da Silva","sequence":"additional","affiliation":[{"name":"Servi\u00e7o de Atendimento Especializado (SAE) Santa Marta, Secretaria Municipal de Sa\u00fade de Porto Alegre , Porto Alegre, RS , Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Karen Oliveira","family":"Furlanetto","sequence":"additional","affiliation":[{"name":"Servi\u00e7o de Atendimento Especializado (SAE) Santa Marta, Secretaria Municipal de Sa\u00fade de Porto Alegre , Porto Alegre, RS , Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alana Witt","family":"Hansen","sequence":"additional","affiliation":[{"name":"Instituto de Ci\u00eancias da Sa\u00fade, Laborat\u00f3rio de Microbiologia 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