{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,18]],"date-time":"2026-09-18T22:45:23Z","timestamp":1789771523500,"version":"4.0.1"},"reference-count":22,"publisher":"Oxford University Press (OUP)","issue":"2","license":[{"start":{"date-parts":[[2022,12,13]],"date-time":"2022-12-13T00:00:00Z","timestamp":1670889600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"funder":[{"name":"French National Reference Centre for staphylococci"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2023,2,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:sec>\n                  <jats:title>Objectives<\/jats:title>\n                  <jats:p>To describe two linezolid-resistant MRSA strains carrying the cfr(B) gene detected in the French National Reference Centre for staphylococci.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Methods<\/jats:title>\n                  <jats:p>Two linezolid-resistant MRSA strains isolated from cystic fibrosis patients in two different French hospitals in 2017 and 2019 were examined to explore the mechanisms of linezolid resistance. Antimicrobial susceptibility was tested using broth microdilution and gradient strips. The genetic determinants of linezolid resistance were assessed by a multiplex PCR targeting cfr\/cfr(B), optrA and poxtA genes, by amplification and sequencing of individual 23S rRNA genes and by WGS using both Illumina and Nanopore technologies.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Results<\/jats:title>\n                  <jats:p>The two MRSA strains were resistant to linezolid but susceptible to tedizolid, and PCR-positive for cfr\/cfr(B). The WGS analysis indicated that they belonged to two different STs (ST8-MRSA-IV and ST5382-MRSA-IV) and that they both harboured the cfr(B) gene on the same 9.7 kb Tn6218-like chromosomal transposon, a finding only previously reported in Enterococcus sp. and Clostridioides difficile.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Conclusions<\/jats:title>\n                  <jats:p>To the best of our knowledge, this is the first description of the presence of cfr(B) in staphylococci, more specifically in linezolid-resistant MRSA strains. This finding illustrates the risk of horizontal intergenus transfer of oxazolidinone resistance genes in Staphylococcus aureus and highlights the need to monitor such emergence in this species.<\/jats:p>\n               <\/jats:sec>","DOI":"10.1093\/jac\/dkac411","type":"journal-article","created":{"date-parts":[[2022,12,13]],"date-time":"2022-12-13T02:26:41Z","timestamp":1670898401000},"page":"445-449","source":"Crossref","is-referenced-by-count":13,"title":["Linezolid resistance: detection of the <i>cfr<\/i>(B) gene in French clinical MRSA strains"],"prefix":"10.1093","volume":"78","author":[{"given":"Benjamin","family":"Youenou","sequence":"first","affiliation":[{"name":"Hospices Civils de Lyon, Centre National de R\u00e9f\u00e9rence des Staphylocoques, Institut des Agents Infectieux , Lyon F-69004 , France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Patricia","family":"Martins Simoes","sequence":"additional","affiliation":[{"name":"Hospices Civils de Lyon, Centre National de R\u00e9f\u00e9rence des Staphylocoques, Institut des Agents Infectieux , Lyon F-69004 , France"},{"name":"Equipe Pathog\u00e9nie des staphylocoques, CIRI, Centre International de Recherche en Infectiologie, Universit\u00e9 de Lyon, Inserm U1111, Universit\u00e9 Claude Bernard Lyon 1, CNRS UMR5308 , ENS de Lyon , France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anne","family":"Tristan","sequence":"additional","affiliation":[{"name":"Hospices Civils de Lyon, Centre National de R\u00e9f\u00e9rence des Staphylocoques, Institut des Agents Infectieux , Lyon F-69004 , France"},{"name":"Equipe Pathog\u00e9nie des staphylocoques, CIRI, Centre International de Recherche en Infectiologie, Universit\u00e9 de Lyon, Inserm U1111, Universit\u00e9 Claude Bernard Lyon 1, CNRS UMR5308 , ENS de Lyon , France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eric","family":"Farfour","sequence":"additional","affiliation":[{"name":"H\u00f4pital Foch, Service de Biologie clinique , Suresnes F-92150 , France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cl\u00e9mence","family":"Beauruelle","sequence":"additional","affiliation":[{"name":"University Brest, INSERM, EFS, UMR 1078, GGB , Brest F-29200 , France"},{"name":"Department of Bacteriology, Virology, Brest University Hospital, Hospital Hygiene, and Parasitology-Mycology , Brest F-29200 , France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Camille","family":"Kolenda","sequence":"additional","affiliation":[{"name":"Hospices Civils de Lyon, Centre National de R\u00e9f\u00e9rence des Staphylocoques, Institut des Agents Infectieux , Lyon F-69004 , France"},{"name":"Equipe Pathog\u00e9nie des staphylocoques, CIRI, Centre International de Recherche en Infectiologie, Universit\u00e9 de Lyon, Inserm U1111, Universit\u00e9 Claude Bernard Lyon 1, CNRS UMR5308 , ENS de Lyon , France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anne-Ga\u00eblle","family":"Ranc","sequence":"additional","affiliation":[{"name":"Hospices Civils de Lyon, Centre National de R\u00e9f\u00e9rence des Staphylocoques, Institut des Agents Infectieux , Lyon F-69004 , France"},{"name":"Equipe Pathog\u00e9nie des staphylocoques, CIRI, Centre International de Recherche en Infectiologie, Universit\u00e9 de Lyon, Inserm U1111, Universit\u00e9 Claude Bernard Lyon 1, CNRS UMR5308 , ENS de Lyon , France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9412-7106","authenticated-orcid":false,"given":"Fran\u00e7ois","family":"Vandenesch","sequence":"additional","affiliation":[{"name":"Hospices Civils de Lyon, Centre National de R\u00e9f\u00e9rence des Staphylocoques, Institut des Agents Infectieux , Lyon F-69004 , France"},{"name":"Equipe Pathog\u00e9nie des staphylocoques, CIRI, Centre International de Recherche en Infectiologie, 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