{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,20]],"date-time":"2026-07-20T22:04:39Z","timestamp":1784585079059,"version":"3.55.0"},"reference-count":35,"publisher":"Oxford University Press (OUP)","issue":"2","license":[{"start":{"date-parts":[[2022,12,28]],"date-time":"2022-12-28T00:00:00Z","timestamp":1672185600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2023,2,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:sec>\n                  <jats:title>Background<\/jats:title>\n                  <jats:p>Vancomycin and linezolid resistance among enterococci is an increasing problem due to a lack of alternative antibiotics. Early identification of vancomycin-resistant and linezolid-resistant strains can help prevent the spread of resistance to these antibiotics. Hence, early, rapid and accurate detection of vancomycin and linezolid resistance is critical.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Objectives<\/jats:title>\n                  <jats:p>The resazurin microplate method (RMM) was developed for detecting vancomycin and linezolid susceptibility among Enterococcus faecalis (E. faecalis) and Enterococcus faecium (E. faecium) clinical isolates, and its performance was further evaluated.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Methods<\/jats:title>\n                  <jats:p>A total of 209 non-duplicate clinical isolates and three strains from the faeces of domestic animals, including 142 E. faecalis (71 linezolid non-susceptible and 71 linezolid susceptible) and 70 E. faecium (23 vancomycin non-susceptible, 23 vancomycin susceptible, 12 linezolid non-susceptible and 12 linezolid susceptible), were tested using RMM.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Results<\/jats:title>\n                  <jats:p>The susceptibility of E. faecium to vancomycin was detected within 5 h, with high susceptibility (23\/23) and specificity (23\/23). The susceptibility of E. faecalis and E. faecium to linezolid was detected within 4 h, with specificities of 98.59% and 100% and susceptibilities of 94.37% and 58.33% for E. faecalis and E. faecium, respectively.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Conclusions<\/jats:title>\n                  <jats:p>RMM had a good positive predictive value for the detection of vancomycin-non-susceptible E. faecium and linezolid-non-susceptible E. faecalis. It thus has the potential to become an alternative method for the rapid screening of these resistant pathogens in clinical practice.<\/jats:p>\n               <\/jats:sec>","DOI":"10.1093\/jac\/dkac415","type":"journal-article","created":{"date-parts":[[2022,12,28]],"date-time":"2022-12-28T04:21:50Z","timestamp":1672201310000},"page":"466-477","source":"Crossref","is-referenced-by-count":4,"title":["Evaluation of resazurin microplate method for rapid detection of vancomycin and linezolid resistance in <i>Enterococcus faecalis and Enterococcus faecium<\/i> clinical isolates"],"prefix":"10.1093","volume":"78","author":[{"given":"Weiliang","family":"Zeng","sequence":"first","affiliation":[{"name":"Department of Clinical Laboratory, The First Affiliated Hospital of Wenzhou Medical University, Key Laboratory of Clinical Laboratory Diagnosis and Translational Research of Zhejiang Province , Wenzhou, Zhejiang Province , China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yijia","family":"Han","sequence":"additional","affiliation":[{"name":"Department of Medical Lab Science, School of Laboratory Medicine and Life Science, Wenzhou Medical University , Wenzhou, Zhejiang Province , China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiangkuo","family":"Zheng","sequence":"additional","affiliation":[{"name":"Department of Clinical Laboratory, The First Affiliated Hospital of Wenzhou Medical University, Key Laboratory of Clinical Laboratory Diagnosis and Translational Research of Zhejiang Province , Wenzhou, Zhejiang Province , China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhuocheng","family":"Yao","sequence":"additional","affiliation":[{"name":"Department of Medical Lab Science, School of Laboratory Medicine and Life Science, Wenzhou Medical University , Wenzhou, Zhejiang Province , China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chunquan","family":"Xu","sequence":"additional","affiliation":[{"name":"Department of Clinical Laboratory, The First Affiliated Hospital of Wenzhou Medical University, Key Laboratory of Clinical Laboratory Diagnosis and Translational Research of Zhejiang Province , Wenzhou, Zhejiang Province , China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaotuan","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Clinical Laboratory, The First Affiliated Hospital of Wenzhou Medical University, Key Laboratory of Clinical Laboratory Diagnosis and Translational Research of Zhejiang Province , Wenzhou, Zhejiang Province , China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Miran","family":"Tang","sequence":"additional","affiliation":[{"name":"Department of Clinical Laboratory, The First Affiliated Hospital of Wenzhou Medical University, Key Laboratory of Clinical Laboratory Diagnosis and Translational Research of Zhejiang Province , Wenzhou, Zhejiang Province , China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mo","family":"Shen","sequence":"additional","affiliation":[{"name":"Department of Clinical Laboratory, The First Affiliated Hospital of Wenzhou Medical University, Key Laboratory of Clinical Laboratory Diagnosis and Translational Research of Zhejiang Province , Wenzhou, Zhejiang Province , China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2171-4710","authenticated-orcid":false,"given":"Tieli","family":"Zhou","sequence":"additional","affiliation":[{"name":"Department of Clinical Laboratory, The First Affiliated Hospital of Wenzhou Medical University, Key Laboratory of Clinical Laboratory Diagnosis and Translational Research of Zhejiang Province , Wenzhou, Zhejiang Province , China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2022,12,28]]},"reference":[{"key":"2023020108211221800_dkac415-B1","doi-asserted-by":"crossref","first-page":"579806","DOI":"10.3389\/fmicb.2021.579806","article-title":"Drug repurposing: in vitro and in vivo antimicrobial and antibiofilm effects of bithionol against Enterococcus faecalis and Enterococcus faecium","volume":"12","author":"She","year":"2021","journal-title":"Front Microbiol"},{"key":"2023020108211221800_dkac415-B2","doi-asserted-by":"crossref","first-page":"3222","DOI":"10.1093\/eurheartj\/ehz620","article-title":"Clinical presentation, aetiology and outcome of infective endocarditis. 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