{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,27]],"date-time":"2026-05-27T23:56:17Z","timestamp":1779926177481,"version":"3.53.1"},"reference-count":32,"publisher":"Oxford University Press (OUP)","issue":"2","license":[{"start":{"date-parts":[[2022,12,28]],"date-time":"2022-12-28T00:00:00Z","timestamp":1672185600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"funder":[{"DOI":"10.13039\/501100004608","name":"Natural Science Foundation of Jiangsu Province","doi-asserted-by":"crossref","award":["BK20191210"],"award-info":[{"award-number":["BK20191210"]}],"id":[{"id":"10.13039\/501100004608","id-type":"DOI","asserted-by":"crossref"}]},{"name":"Scientific Research Project in Jiangsu Province","award":["BRA2019248"],"award-info":[{"award-number":["BRA2019248"]}]},{"DOI":"10.13039\/100017962","name":"Jiangsu Commission of Health","doi-asserted-by":"crossref","award":["H2018073"],"award-info":[{"award-number":["H2018073"]}],"id":[{"id":"10.13039\/100017962","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2023,2,1]]},"abstract":"Abstract<\/jats:title>\n \n Background<\/jats:title>\n Klebsiella pneumoniae carbapenemase (KPC)-producing K. pneumoniae (KPC-KP) has spread worldwide and has become a major threat to public health. The restriction modification system provides an innate defence of bacteria against plasmids or transposons, while many different types of plasmid encoding the anti-restriction protein ArdA can specifically affect the restriction activity in bacteria.<\/jats:p>\n <\/jats:sec>\n \n Objectives<\/jats:title>\n To detect the codistribution of ArdA and blaKPC-2 plasmids in KPC-KP and explore the molecular mechanism of ArdA promoting KPC-KP spread.<\/jats:p>\n <\/jats:sec>\n \n Methods<\/jats:title>\n We collected 65 clinical CRKP isolates from Ningbo, China, and 68 cases of plasmid complete sequences in GenBank to determine the prevalence of ArdA gene on the K. pneumoniae blaKPC-2 plasmid. The anti-restriction function of ArdA in promoting horizontal gene transfer (HGT) was verified by transformation, conjugation and transduction methods, and the pull-down experiment was used to investigate the molecular mechanism of ArdA protein in vitro.<\/jats:p>\n <\/jats:sec>\n \n Results<\/jats:title>\n We found that ArdA was widely distributed in KPC-KP in 100% of cases, which was detected in 0% of drug susceptible K. pneumoniae, and the plasmids containing the ArdA gene in 90% of the 30 cases randomly retrieved from the database. We also verified that ArdA has a good anti-restriction function (P\u200a&lt;\u200a0.05) through two aspects of HGT (transformation, transduction), and explored the non-occurrence interaction of ArdA and the hsdM subunit protein of EcoKI enzyme from the perspective of protein molecules.<\/jats:p>\n <\/jats:sec>\n \n Conclusions<\/jats:title>\n These findings suggest that the coexistence advantage of ArdA with the blaKPC-2 plasmids may provide KPC-producing K. pneumoniae with a very efficient evasion of the restriction of type I systems, which not only favours ArdA-containing mobile genetic elements in the same species HGT between bacteria also facilitates HGT between other bacterial species.<\/jats:p>\n <\/jats:sec>","DOI":"10.1093\/jac\/dkac423","type":"journal-article","created":{"date-parts":[[2022,12,28]],"date-time":"2022-12-28T04:31:50Z","timestamp":1672201910000},"page":"521-530","source":"Crossref","is-referenced-by-count":3,"title":["Anti-restriction protein ArdA promotes clinical Klebsiella pneumoniae<\/i> carbapenemase (KPC)-producing K. pneumoniae<\/i> spread and its molecular mechanism"],"prefix":"10.1093","volume":"78","author":[{"given":"Huimin","family":"Chen","sequence":"first","affiliation":[{"name":"School of Medicine, Jiangsu University , Zhenjiang, Jiangsu , China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shuan","family":"Tao","sequence":"additional","affiliation":[{"name":"School of Medicine, Jiangsu University , Zhenjiang, Jiangsu , China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Na","family":"Li","sequence":"additional","affiliation":[{"name":"Laboratory Medical School, Bengbu Medical College , Bengbu, Anhui , China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qing","family":"Zhu","sequence":"additional","affiliation":[{"name":"School of Medicine, Xuzhou Medical University , Xuzhou, Jiangsu , 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