{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,4]],"date-time":"2026-09-04T23:34:01Z","timestamp":1788564841707,"version":"build-2803163510"},"reference-count":21,"publisher":"Oxford University Press (OUP)","issue":"3","license":[{"start":{"date-parts":[[2023,1,6]],"date-time":"2023-01-06T00:00:00Z","timestamp":1672963200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"funder":[{"DOI":"10.13039\/501100004718","name":"Toulouse University","doi-asserted-by":"crossref","id":[{"id":"10.13039\/501100004718","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2023,3,2]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>A new mutation in the Plasmodium falciparum dihydropteroate synthetase gene (pfdhps), I431V, has been identified in several countries of Central and West Africa. This mutation is mostly found in association with four other SNPs on pfdhps (S436A, A437G, A581G and A613S), forming a quintuple mutant (vagKgs) and almost always associated with the Plasmodium falciparum dihydrofolate reductase gene (pfdhfr) CirnI (C50R, N51I, S108N) triple mutant. To date, nothing is known about the impact of this new pfdhps genotype on sulfadoxine-pyrimethamine (SP) resistance.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Objectives<\/jats:title>\n                    <jats:p>We sought to assess the prevalence of this pfdhps vagKgs quintuple mutant in two groups of pregnant women with malaria, one that took intermittent preventive treatment with sulfadoxine-pyrimethamine (IPTp-SP) and one that did not.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>The pfdhfr and pfdhps genes from Plasmodium falciparum isolates collected in Yaound\u00e9 (Cameroon) from pregnant women with symptomatic malaria under IPTp-SP or not, were sequenced.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>Of 159 patients evaluated, 70 had already taken SP during pregnancy and 89 had never taken SP. Only the vagKgs allele was significantly overrepresented in the SP+ group (21.4% versus 3.4%; P\u200a&amp;lt;\u200a0.001), whereas the ISgKAA mutant, widely distributed in this area and known to be less susceptible to SP, tended to be less abundant in this group (48.6% versus 64.0%; P\u200a=\u200a0.0503).<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>We found a strong overrepresentation of the CirnI\/vagKgs haplotype in the IPTp-SP pregnant group, suggesting a high level of resistance of this mutant to SP. This could compromise not only the effectiveness of IPTp-SP but also the seasonal malaria chemoprevention of young children, now widely implemented.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1093\/jac\/dkac432","type":"journal-article","created":{"date-parts":[[2022,12,16]],"date-time":"2022-12-16T18:07:20Z","timestamp":1671214040000},"page":"665-668","source":"Crossref","is-referenced-by-count":15,"title":["Effect of sulfadoxine-pyrimethamine chemoprophylaxis in pregnant women on selection of the new\n                    <i>P. falciparum dhps<\/i>\n                    quintuple mutant carrying the I431V mutation"],"prefix":"10.1093","volume":"78","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5522-9003","authenticated-orcid":false,"given":"Olivia","family":"Cohen","sequence":"first","affiliation":[{"name":"Service de Parasitologie-Mycologie, CHU Toulouse , Toulouse , France"},{"name":"Toulouse Institute for Infectious and Inflammatory Diseases (Infinity), CNRS UMR5051, INSERM UMR1291, UPS , Toulouse , France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Emilie","family":"Guemas","sequence":"additional","affiliation":[{"name":"Service de Parasitologie-Mycologie, CHU Toulouse , Toulouse , France"},{"name":"Toulouse Institute for Infectious and Inflammatory Diseases (Infinity), CNRS UMR5051, INSERM UMR1291, UPS , Toulouse , France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sandie","family":"Menard","sequence":"additional","affiliation":[{"name":"Toulouse Institute for Infectious and Inflammatory Diseases (Infinity), CNRS UMR5051, INSERM UMR1291, UPS , Toulouse , France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Martial","family":"Tsague Kenfack","sequence":"additional","affiliation":[{"name":"Centre d\u2019Animation Sociale et Sanitaire (CASS) of Nkolndongo , Yaounde , Cameroon"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carine","family":"Talom Ngassa","sequence":"additional","affiliation":[{"name":"Centre d\u2019Animation Sociale et Sanitaire (CASS) of Nkolndongo , Yaounde , Cameroon"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xavier","family":"Iriart","sequence":"additional","affiliation":[{"name":"Service de Parasitologie-Mycologie, CHU Toulouse , Toulouse , France"},{"name":"Toulouse Institute for Infectious and Inflammatory Diseases (Infinity), CNRS UMR5051, INSERM UMR1291, UPS , Toulouse , France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marlise","family":"Bidzogo Lebobo","sequence":"additional","affiliation":[{"name":"Centre d\u2019Animation Sociale et Sanitaire (CASS) of Nkolndongo , Yaounde , Cameroon"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Celestin","family":"Ondobo Ekae","sequence":"additional","affiliation":[{"name":"Centre d\u2019Animation Sociale et Sanitaire (CASS) of Nkolndongo , Yaounde , Cameroon"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carole","family":"Eboumbou","sequence":"additional","affiliation":[{"name":"Facult\u00e9 de M\u00e9decine et des Sciences Pharmaceutiques, Universit\u00e9 de Douala , Douala , Cameroon"},{"name":"Malaria Research Unit, Centre Pasteur du Cameroun , Yaound\u00e9 , Cameroon"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Calvin","family":"Tiyou Kenmeni","sequence":"additional","affiliation":[{"name":"Centre d\u2019Animation Sociale et Sanitaire (CASS) of Nkolndongo , Yaounde , Cameroon"},{"name":"University Hospital of Yaound\u00e9 , Yaound\u00e9 , Cameroon"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6626-7766","authenticated-orcid":false,"given":"Antoine","family":"Berry","sequence":"additional","affiliation":[{"name":"Service de Parasitologie-Mycologie, CHU Toulouse , Toulouse , France"},{"name":"Toulouse Institute for Infectious and Inflammatory Diseases (Infinity), CNRS UMR5051, INSERM UMR1291, UPS , Toulouse , France"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2023,1,6]]},"reference":[{"key":"2023030208085615900_","doi-asserted-by":"crossref","first-page":"107","DOI":"10.1016\/S1473-3099(18)30066-5","article-title":"Burden, pathology, and costs of malaria in pregnancy: new developments for an old problem","volume":"18","author":"Rogerson","year":"2018","journal-title":"Lancet Infect Dis"},{"key":"2023030208085615900_","author":"World Health Organization","year":"2013"},{"key":"2023030208085615900_","doi-asserted-by":"crossref","first-page":"9114","DOI":"10.1073\/pnas.85.23.9114","article-title":"Evidence that a point mutation in dihydrofolate reductase-thymidylate synthase confers resistance to pyrimethamine in falciparum malaria","volume":"85","author":"Peterson","year":"1988","journal-title":"Proc Natl Acad Sci U S A"},{"key":"2023030208085615900_","doi-asserted-by":"crossref","first-page":"13944","DOI":"10.1073\/pnas.94.25.13944","article-title":"Mutations in dihydropteroate synthase are responsible for sulfone and sulfonamide resistance in Plasmodium falciparum","volume":"94","author":"Triglia","year":"1997","journal-title":"Proc Natl Acad Sci U S A"},{"issue":"Suppl 1","key":"2023030208085615900_","doi-asserted-by":"crossref","first-page":"11","DOI":"10.1016\/j.trstmh.2008.11.002","article-title":"The evolution of drug-resistant malaria","volume":"103","author":"Plowe","year":"2009","journal-title":"Trans R Soc Trop Med Hyg"},{"key":"2023030208085615900_","doi-asserted-by":"crossref","first-page":"3405","DOI":"10.1128\/AAC.00024-09","article-title":"Novel pfdhps haplotypes among imported cases of Plasmodium falciparum malaria in the United Kingdom","volume":"53","author":"Sutherland","year":"2009","journal-title":"Antimicrob Agents Chemother"},{"key":"2023030208085615900_","doi-asserted-by":"crossref","first-page":"2566","DOI":"10.1093\/jac\/dkv160","article-title":"Prevalence of Plasmodium falciparum parasites resistant to sulfadoxine\/pyrimethamine in pregnant women in Yaound\u00e9, Cameroon: emergence of highly resistant pfdhfr\/pfdhps alleles","volume":"70","author":"Chauvin","year":"2015","journal-title":"J Antimicrob Chemother"},{"key":"2023030208085615900_","article-title":"Molecular determinants of sulfadoxine-pyrimethamine resistance in Plasmodium falciparum isolates from Central Africa between 2016 and 2021: wide geographic spread of highly mutated Pfdhfr and Pfdhps alleles","volume":"10","author":"Wang","year":"2022","journal-title":"Microbiol Spectr"},{"key":"2023030208085615900_","doi-asserted-by":"crossref","first-page":"505","DOI":"10.1016\/j.pt.2013.08.002","article-title":"Mapping \u201cpartially resistant\u201d, \u201cfully resistant\u201d, and \u201csuper resistant\u201d malaria","volume":"29","author":"Naidoo","year":"2013","journal-title":"Trends Parasitol"},{"key":"2023030208085615900_","doi-asserted-by":"crossref","DOI":"10.3389\/fped.2022.966402","article-title":"Prevention of malaria in pregnancy: the threat of sulfadoxine-pyrimethamine resistance","volume":"10","author":"Sundararaman","year":"2022","journal-title":"Front Pediatr"},{"key":"2023030208085615900_","doi-asserted-by":"crossref","first-page":"1163","DOI":"10.1056\/NEJMoa2101746","article-title":"Evidence of artemisinin-resistant malaria in Africa","volume":"385","author":"Balikagala","year":"2021","journal-title":"N Engl J Med"},{"key":"2023030208085615900_","author":"Whatman"},{"key":"2023030208085615900_","doi-asserted-by":"crossref","first-page":"1066","DOI":"10.1016\/S0140-6736(01)06201-8","article-title":"Plasmodium falciparum cross-resistance between trimethoprim and pyrimethamine","volume":"358","author":"Iyer","year":"2001","journal-title":"Lancet"},{"key":"2023030208085615900_","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pntd.0007223","article-title":"The prevalence and antifolate drug resistance profiles of Plasmodium falciparum in study participants randomized to discontinue or continue cotrimoxazole prophylaxis","volume":"13","author":"Juma","year":"2019","journal-title":"PLoS Negl Trop Dis"},{"key":"2023030208085615900_","doi-asserted-by":"crossref","first-page":"531","DOI":"10.1093\/trstmh\/trab061","article-title":"Antimalarial drug resistance markers in human immunodeficiency virus (HIV)-positive and HIV-negative adults with asymptomatic malaria infections in Port Harcourt, Nigeria","volume":"115","author":"Chijioke-Nwauche","year":"2021","journal-title":"Trans R Soc Trop Med Hyg"},{"key":"2023030208085615900_","doi-asserted-by":"crossref","DOI":"10.1128\/aac.00188-22","article-title":"Genetic diversity of Plasmodium falciparum and distribution of antimalarial drug resistance mutations in symptomatic and asymptomatic infections","volume":"66","author":"Sarah-Matio","year":"2022","journal-title":"Antimicrob Agents Chemother"},{"key":"2023030208085615900_","article-title":"Within-host competition and drug resistance in the human malaria parasite Plasmodium falciparum","volume":"283","author":"Bushman","year":"2016","journal-title":"Proc Biol Sci"},{"key":"2023030208085615900_","doi-asserted-by":"crossref","first-page":"993","DOI":"10.1086\/315787","article-title":"Rapid selection of Plasmodium falciparum dihydrofolate reductase mutants by pyrimethamine prophylaxis","volume":"182","author":"Doumbo","year":"2000","journal-title":"J Infect Dis"},{"key":"2023030208085615900_","doi-asserted-by":"crossref","first-page":"775","DOI":"10.1093\/infdis\/jiz510","article-title":"Multiplicity of asymptomatic Plasmodium falciparum infections and risk of clinical malaria: a systematic review and pooled analysis of individual participant data","volume":"221","author":"Eldh","year":"2020","journal-title":"J Infect Dis"},{"key":"2023030208085615900_","doi-asserted-by":"crossref","DOI":"10.1128\/Spectrum.00852-21","article-title":"The rare, the best: spread of antimalarial-resistant Plasmodium falciparum parasites by Anopheles mosquito vectors","volume":"9","author":"Berry","year":"2021","journal-title":"Microbiol Spectr"},{"key":"2023030208085615900_","doi-asserted-by":"crossref","first-page":"1829","DOI":"10.1016\/S0140-6736(20)32227-3","article-title":"Effectiveness of seasonal malaria chemoprevention at scale in west and Central Africa: an observational study","volume":"396","author":"ACCESS-SMC Partnership","year":"2020","journal-title":"Lancet"}],"container-title":["Journal of Antimicrobial Chemotherapy"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/academic.oup.com\/jac\/article-pdf\/78\/3\/665\/49376278\/dkac432.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/academic.oup.com\/jac\/article-pdf\/78\/3\/665\/49376278\/dkac432.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,3,18]],"date-time":"2023-03-18T04:06:58Z","timestamp":1679112418000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/jac\/article\/78\/3\/665\/6972437"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,1,6]]},"references-count":21,"journal-issue":{"issue":"3","published-online":{"date-parts":[[2023,1,6]]},"published-print":{"date-parts":[[2023,3,2]]}},"URL":"https:\/\/doi.org\/10.1093\/jac\/dkac432","relation":{},"ISSN":["0305-7453","1460-2091"],"issn-type":[{"value":"0305-7453","type":"print"},{"value":"1460-2091","type":"electronic"}],"subject":[],"published-other":{"date-parts":[[2023,3,2]]},"published":{"date-parts":[[2023,1,6]]}}}