{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,10]],"date-time":"2026-09-10T22:40:52Z","timestamp":1789080052989,"version":"build-2803163510"},"reference-count":36,"publisher":"Oxford University Press (OUP)","issue":"4","license":[{"start":{"date-parts":[[2023,3,8]],"date-time":"2023-03-08T00:00:00Z","timestamp":1678233600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"crossref","award":["31902340"],"award-info":[{"award-number":["31902340"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"crossref"}]},{"name":"National Center of Technology Innovation for Pigs","award":["NCTIP-XD\/B11"],"award-info":[{"award-number":["NCTIP-XD\/B11"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2023,4,3]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Objectives<\/jats:title><jats:p>To elucidate the role of a novel carbapenem-hydrolysing class D \u03b2-lactamase (RAD-1) from Riemerella anatipestifer.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>We applied WGS and bioinformatic analysis to screen putative \u03b2-lactamase genes in R. anatipestifer SCVM0004. A putative class D \u03b2-lactamase gene was cloned into pET24a and transferred into Escherichia coli BL21 (DE3) for antibiotic susceptibility determination and protein purification. Meanwhile, the purified native protein was used to determine the enzymatic activities.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>A class D \u03b2-lactamase, RAD-1, was identified in the genome of R. anatipestifer SCVM0004. It was distinct from all characterized class D \u03b2-lactamases (\u226442% amino acid sequence identity). Searching in GenBank showed that blaRAD-1 was widely disseminated among R. anatipestifer. Genomic environment analysis indicated that the chromosomal structures of blaRAD-1-located regions were relatively conserved. Expression of RAD-1 in E. coli results in elevated MICs for various \u03b2-lactam antibiotics, including penicillins, extended-spectrum cephalosporins, a monobactam and carbapenems. Moreover, kinetic analysis of purified RAD-1 revealed: (i) high-level activity against penicillins; (ii) highest affinity for carbapenems; (iii) moderate hydrolysis of extended-spectrum cephalosporins and a monobactam; and (iv) no activity for oxacillin and cefoxitin.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>This study identified a novel chromosomally located class D carbapenemase RAD-1 (Bush\u2013Jacoby functional group 2def) in R. anatipestifer SCVM0004. Moreover, bioinformatic analysis confirmed that the RAD-1 was widely prevalent and conserved in R. anatipestifer.<\/jats:p><\/jats:sec>","DOI":"10.1093\/jac\/dkad058","type":"journal-article","created":{"date-parts":[[2023,3,8]],"date-time":"2023-03-08T12:27:55Z","timestamp":1678278475000},"page":"1117-1124","source":"Crossref","is-referenced-by-count":11,"title":["Identification of a novel carbapenem-hydrolysing class D \u03b2-lactamase RAD-1 in<i>Riemerella anatipestifer<\/i>"],"prefix":"10.1093","volume":"78","author":[{"given":"Pei","family":"Li","sequence":"first","affiliation":[{"name":"College of Veterinary Medicine, Southwest University , Beibei, Chongqing , China"},{"name":"National Center of Technology Innovation for Pigs , Rongchang, Chongqing , China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhishuang","family":"Yang","sequence":"additional","affiliation":[{"name":"Research Center of Avian Diseases, College of Veterinary Medicine, Sichuan Agricultural 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