{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,16]],"date-time":"2026-07-16T01:11:46Z","timestamp":1784164306874,"version":"3.55.0"},"reference-count":32,"publisher":"Oxford University Press (OUP)","issue":"6","license":[{"start":{"date-parts":[[2023,4,11]],"date-time":"2023-04-11T00:00:00Z","timestamp":1681171200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100003593","name":"National Council for Science and Technological Development","doi-asserted-by":"publisher","award":["312066\/2019"],"award-info":[{"award-number":["312066\/2019"]}],"id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2023,6,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:sec>\n                  <jats:title>Objectives<\/jats:title>\n                  <jats:p>To characterize a novel acquired MBL, BIM-1, in a Pseudomonas #2 (subgroup P. guariconensis) strain isolated from the Aur\u00e1 river located in the Brazilian Amazon hydrographic basin.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Methods<\/jats:title>\n                  <jats:p>WGS using an Illumina\u00ae MiSeq System was used to characterize the genome of Pseudomonas sp. IEC33019 strain. Southern blotting\/hybridization assays were performed to confirm the location of the MBL-encoding gene, blaBIM-1 (Bel\u00e9m Imipenemase). Antimicrobial susceptibility testing, cloning, and biochemical and phenotypic characterization were performed to determine BIM-1 kinetics.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Results<\/jats:title>\n                  <jats:p>The IEC33019 strain showed high resistance rates to \u03b2-lactams, ciprofloxacin and aminoglycosides, being susceptible only to polymyxins and susceptible, increased exposure to aztreonam. WGS analysis revealed a novel acquired MBL-encoding gene, blaBIM-1, found as a gene cassette inserted into a class 1 integron (In1326) that also carried qnrVC1 and aadA11e. In1326 was located in a complex transposon, Tn7122, carried by a 52.7 kb conjugative plasmid (pIEC33019) with a toxin\/antitoxin system (vapB\/vapC). BIM-1 belongs to the molecular subgroup B1 and shares 70.2% and 64.9% similarity with SIM-1 and IMP-1, respectively. Kinetics analysis of BIM-1 showed hydrolytic activity against all \u03b2-lactams tested.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Conclusions<\/jats:title>\n                  <jats:p>BIM-1 is a novel acquired MBL encoded by a gene carried by mobile genetic elements, which can be transferred to other Gram-negative bacilli (GNB). Because the IEC33019 strain was recovered from a river impacted by a populous metropolitan region with poor basic sanitation and served by limited potable freshwater, it would be important to establish the role of the BIM-1-producing GNB as nosocomial pathogens and\/or as colonizers of the riverside population in this geographical region.<\/jats:p>\n               <\/jats:sec>","DOI":"10.1093\/jac\/dkad077","type":"journal-article","created":{"date-parts":[[2023,4,11]],"date-time":"2023-04-11T08:49:21Z","timestamp":1681202961000},"page":"1359-1366","source":"Crossref","is-referenced-by-count":11,"title":["Genetic and biochemical characterization of BIM-1, a novel acquired subgroup B1 MBL found in a <i>Pseudomonas<\/i> sp. strain from the Brazilian Amazon region"],"prefix":"10.1093","volume":"78","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5496-3713","authenticated-orcid":false,"given":"Cintya O","family":"Souza","sequence":"first","affiliation":[{"name":"Se\u00e7\u00e3o de Bacteriologia e Micologia, Instituto Evandro Chagas (IEC) , Ananindeua, PA , Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rodrigo","family":"Cay\u00f4","sequence":"additional","affiliation":[{"name":"Universidade Federal de S\u00e3o Paulo (UNIFESP), Laborat\u00f3rio ALERTA, Disciplina de Infectologia, Departamento de Medicina, Escola Paulista de Medicina (EPM) , S\u00e3o Paulo, SP , Brazil"},{"name":"Universidade Federal de S\u00e3o Paulo (UNIFESP), Laborat\u00f3rio de Bacteriologia e Imunologia (LIB), Setor de Biologia Molecular, Microbiologia e Imunologia, Departamento de Ci\u00eancias Biol\u00f3gicas (DCB), Instituto de Ci\u00eancias Ambientais, Qu\u00edmicas e Farmac\u00eauticas (ICAQF) , Diadema, SP , Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Karla Val\u00e9ria B","family":"Lima","sequence":"additional","affiliation":[{"name":"Se\u00e7\u00e3o de Bacteriologia e Micologia, Instituto Evandro Chagas (IEC) , Ananindeua, PA , Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Danielle M","family":"Brasiliense","sequence":"additional","affiliation":[{"name":"Se\u00e7\u00e3o de Bacteriologia e Micologia, Instituto Evandro Chagas (IEC) , Ananindeua, PA , Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ana Paula","family":"Streling","sequence":"additional","affiliation":[{"name":"Universidade Federal de S\u00e3o Paulo (UNIFESP), Laborat\u00f3rio ALERTA, Disciplina de Infectologia, Departamento de Medicina, Escola Paulista de Medicina (EPM) , S\u00e3o Paulo, SP , Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andr\u00e9 V","family":"Siqueira","sequence":"additional","affiliation":[{"name":"Universidade Federal de S\u00e3o Paulo (UNIFESP), Laborat\u00f3rio ALERTA, Disciplina de Infectologia, Departamento de Medicina, Escola Paulista de Medicina (EPM) , S\u00e3o Paulo, SP , Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2855-3147","authenticated-orcid":false,"given":"Felipe","family":"Alberto-Lei","sequence":"additional","affiliation":[{"name":"Universidade Federal de S\u00e3o Paulo (UNIFESP), Laborat\u00f3rio ALERTA, Disciplina de Infectologia, Departamento de Medicina, Escola Paulista de Medicina (EPM) , S\u00e3o Paulo, SP , Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8685-3199","authenticated-orcid":false,"given":"Josiane T","family":"Leal","sequence":"additional","affiliation":[{"name":"Universidade Federal de S\u00e3o Paulo (UNIFESP), Laborat\u00f3rio ALERTA, Disciplina de Infectologia, Departamento de Medicina, Escola Paulista de Medicina (EPM) , S\u00e3o Paulo, SP , Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2361-5732","authenticated-orcid":false,"given":"Carolina S","family":"Nodari","sequence":"additional","affiliation":[{"name":"Universidade Federal de S\u00e3o Paulo (UNIFESP), Laborat\u00f3rio ALERTA, Disciplina de Infectologia, Departamento de Medicina, Escola Paulista de Medicina (EPM) , S\u00e3o Paulo, SP , Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paula Juliana","family":"P\u00e9rez-Chaparro","sequence":"additional","affiliation":[{"name":"Department of Clinical Analysis, School of Pharmacy (FCF), Universidade de S\u00e3o Paulo (USP) , S\u00e3o Paulo, SP , Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Luana N G C","family":"Lima","sequence":"additional","affiliation":[{"name":"Se\u00e7\u00e3o de Bacteriologia e Micologia, Instituto Evandro Chagas (IEC) , Ananindeua, PA , Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marcelo O","family":"Lima","sequence":"additional","affiliation":[{"name":"Se\u00e7\u00e3o de Meio Ambiente, Instituto Evandro Chagas (IEC) , 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