{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,17]],"date-time":"2026-08-17T23:23:51Z","timestamp":1787009031757,"version":"build-2736575974"},"reference-count":33,"publisher":"Oxford University Press (OUP)","issue":"8","license":[{"start":{"date-parts":[[2023,6,9]],"date-time":"2023-06-09T00:00:00Z","timestamp":1686268800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"funder":[{"name":"Agria and SKK Research Fund"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2023,8,2]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Methicillin-resistant Staphylococcus pseudintermedius (MRSP) lineages harbouring staphylococcal cassette chromosome (SCC) mec types IV, V and \u03a8SCCmec57395 usually display low oxacillin MICs (0.5\u20132 mg\/L).<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Objectives<\/jats:title>\n                    <jats:p>To evaluate how oxacillin MICs correlate with PBP mutations and susceptibility to \u03b2-lactams approved for veterinary use.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>Associations between MICs and PBP mutations were investigated by broth microdilution, time\u2013kill and genome sequence analyses in 117 canine MRSP strains harbouring these SCCmec types. Clinical outcome was retrospectively evaluated in 11 MRSP-infected dogs treated with \u03b2-lactams.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>Low-level MRSP was defined by an oxacillin MIC &amp;lt;4 mg\/L. Regardless of strain genotype, all low-level MRSP isolates (n\u200a=\u200a89) were cefalexin susceptible, whereas no strains were amoxicillin\/clavulanate susceptible according to clinical breakpoints. Exposure to 2\u00d7 MIC of cefalexin resulted in complete killing within 8 h. High (\u22654 mg\/L) oxacillin MICs were associated with substitutions in native PBP2, PBP3, PBP4 and acquired PBP2a, one of which (V390M in PBP3) was statistically significant by multivariable modelling. Eight of 11 dogs responded to systemic therapy with first-generation cephalosporins (n\u200a=\u200a4) or amoxicillin\/clavulanate (n\u200a=\u200a4) alone or with concurrent topical treatment, including 6 of 7 dogs infected with low-level MRSP.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>Oxacillin MIC variability in MRSP is influenced by mutations in multiple PBPs and correlates with cefalexin susceptibility. The expert rule recommending that strains with oxacillin MIC \u22650.5 mg\/L are reported as resistant to all \u03b2-lactams should be reassessed based on these results, which are highly clinically relevant in light of the shortage of effective antimicrobials for systemic treatment of MRSP infections in veterinary medicine.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1093\/jac\/dkad182","type":"journal-article","created":{"date-parts":[[2023,5,31]],"date-time":"2023-05-31T23:22:58Z","timestamp":1685575378000},"page":"1909-1920","source":"Crossref","is-referenced-by-count":9,"title":["<i>In vitro<\/i>\n                    and\n                    <i>in vivo<\/i>\n                    susceptibility to cefalexin and amoxicillin\/clavulanate in canine low-level methicillin-resistant\n                    <i>Staphylococcus pseudintermedius<\/i>"],"prefix":"10.1093","volume":"78","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9814-1500","authenticated-orcid":false,"given":"Mattia","family":"Pirolo","sequence":"first","affiliation":[{"name":"Department of Veterinary and Animal Sciences, Faculty of Health and Medical Sciences, University of Copenhagen , Frederiksberg , Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mareliza","family":"Menezes","sequence":"additional","affiliation":[{"name":"Department of Veterinary and Animal Sciences, Faculty of Health and Medical Sciences, University of Copenhagen , Frederiksberg , Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peter","family":"Damborg","sequence":"additional","affiliation":[{"name":"Department of Veterinary and Animal Sciences, Faculty of Health and Medical Sciences, University of Copenhagen , Frederiksberg , Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alice","family":"Wegener","sequence":"additional","affiliation":[{"name":"Department of Biomolecular Health Sciences, Division of Infectious Diseases and Immunology, Faculty of Veterinary Medicine, Utrecht University , Utrecht , The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Birgitta","family":"Duim","sequence":"additional","affiliation":[{"name":"Department of Biomolecular Health Sciences, Division of Infectious Diseases and Immunology, Faculty of Veterinary Medicine, Utrecht University , Utrecht , The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1312-6115","authenticated-orcid":false,"given":"Els","family":"Broens","sequence":"additional","affiliation":[{"name":"Department of Biomolecular Health Sciences, Division of Infectious Diseases and Immunology, Faculty of Veterinary Medicine, Utrecht University , Utrecht , The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lisbeth Rem","family":"Jessen","sequence":"additional","affiliation":[{"name":"Department of Veterinary Clinical Sciences, Faculty of Health and Medical Sciences, University of Copenhagen , Frederiksberg , Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mette","family":"Schj\u00e6rff","sequence":"additional","affiliation":[{"name":"Department of Veterinary Clinical Sciences, Faculty of Health and Medical Sciences, University of Copenhagen , Frederiksberg , Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Luca","family":"Guardabassi","sequence":"additional","affiliation":[{"name":"Department of Veterinary and Animal Sciences, Faculty of Health and Medical Sciences, University of Copenhagen , Frederiksberg , 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