{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,5]],"date-time":"2026-06-05T17:33:28Z","timestamp":1780680808916,"version":"3.54.1"},"reference-count":55,"publisher":"Oxford University Press (OUP)","issue":"8","license":[{"start":{"date-parts":[[2023,6,30]],"date-time":"2023-06-30T00:00:00Z","timestamp":1688083200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"funder":[{"DOI":"10.13039\/501100008771","name":"Animal and Plant Quarantine Agency","doi-asserted-by":"publisher","award":["Z-1543081-2017-18-02"],"award-info":[{"award-number":["Z-1543081-2017-18-02"]}],"id":[{"id":"10.13039\/501100008771","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003624","name":"Ministry of Agriculture, Food, and Rural Affairs, Republic of Korea","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100003624","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2023,8,2]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:sec>\n                  <jats:title>Background<\/jats:title>\n                  <jats:p>Fluoroquinolones (FQs) are potent and broad-spectrum antibiotics commonly used to treat MDR bacterial infections, but bacterial resistance to FQs has emerged and spread rapidly around the world. The mechanisms for FQ resistance have been revealed, including one or more mutations in FQ target genes such as DNA gyrase (gyrA) and topoisomerase IV (parC). Because therapeutic treatments for FQ-resistant bacterial infections are limited, it is necessary to develop novel antibiotic alternatives to minimize or inhibit FQ-resistant bacteria.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Objectives<\/jats:title>\n                  <jats:p>To examine the bactericidal effect of antisense peptide-peptide nucleic acids (P-PNAs) that can block the expression of DNA gyrase or topoisomerase IV in FQ-resistant Escherichia coli (FRE).<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Methods<\/jats:title>\n                  <jats:p>A set of antisense P-PNA conjugates with a bacterial penetration peptide were designed to inhibit the expression of gyrA and parC and were evaluated for their antibacterial activities.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Results<\/jats:title>\n                  <jats:p>Antisense P-PNAs, ASP-gyrA1 and ASP-parC1, targeting the translational initiation sites of their respective target genes significantly inhibited the growth of the FRE isolates. In addition, ASP-gyrA3 and ASP-parC2, which bind to the FRE-specific coding sequence within the gyrA and parC structural genes, respectively, showed selective bactericidal effects against FRE isolates.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Conclusions<\/jats:title>\n                  <jats:p>Our results demonstrate the potential of targeted antisense P-PNAs as antibiotic alternatives against FQ-resistance bacteria.<\/jats:p>\n               <\/jats:sec>","DOI":"10.1093\/jac\/dkad203","type":"journal-article","created":{"date-parts":[[2023,6,30]],"date-time":"2023-06-30T19:58:23Z","timestamp":1688155103000},"page":"2052-2060","source":"Crossref","is-referenced-by-count":4,"title":["Development of antisense peptide-peptide nucleic acids against fluoroquinolone-resistant <i>Escherichia coli<\/i>"],"prefix":"10.1093","volume":"78","author":[{"given":"Se Kye","family":"Kim","sequence":"first","affiliation":[{"name":"College of Veterinary Medicine & Institute of Veterinary Science, Kangwon National University , Chuncheon, Gangwon 24341 , Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jun Bong","family":"Lee","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine & Institute of Veterinary Science, Kangwon National University , Chuncheon, Gangwon 24341 , Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hyung Tae","family":"Lee","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine & Institute of Veterinary Science, Kangwon National University , Chuncheon, Gangwon 24341 , Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dalmuri","family":"Han","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine & Institute of Veterinary Science, Kangwon National University , Chuncheon, Gangwon 24341 , Republic of 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