{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,6]],"date-time":"2026-06-06T04:17:35Z","timestamp":1780719455989,"version":"3.54.1"},"reference-count":19,"publisher":"Oxford University Press (OUP)","issue":"2","license":[{"start":{"date-parts":[[2023,12,28]],"date-time":"2023-12-28T00:00:00Z","timestamp":1703721600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2024,2,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:sec>\n                  <jats:title>Objectives<\/jats:title>\n                  <jats:p>The aims of this study were: (i) to assess the ability of the meropenem screening breakpoint as part of the screening rapid antimicrobial susceptibility testing (sRAST) of EUCAST for the detection of OXA-48 carbapenemase-producing Klebsiella pneumoniae directly from positive blood cultures (BCs); and (ii) to evaluate the inclusion of ertapenem and temocillin discs into the sRAST to enhance the detection of OXA-48-producing isolates.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Methods<\/jats:title>\n                  <jats:p>BC bottles were spiked with a total of 117 K. pneumoniae isolates, including 77 previously characterized OXA-48 producers and 40 non-OXA-48 producers. Disc diffusion assays were directly performed from positive BCs with meropenem (10 \u00b5g), ertapenem (10 \u00b5g) and temocillin (30 \u00b5g) discs, and inhibition zones were manually measured after 4, 6 and 8 h of incubation. The screening cut-off values of sRAST were applied to evaluate their capability in detecting OXA-48-producing isolates. Receiver operating characteristic curves were constructed to illustrate the performance efficacy of the disc diffusion assays to detect OXA-48 producers.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Results<\/jats:title>\n                  <jats:p>The meropenem cut-off values of sRAST only detected 90.91% of the OXA-48-producing isolates after 6 and 8 h of incubation. With the proposed cut-off points for ertapenem [&amp;lt;19 mm (4\/6 h) and &amp;lt;20 mm (8 h)] and temocillin [&amp;lt;10 mm (4 h) and &amp;lt;11 mm (6\/8 h)], all OXA-48-positive isolates were detected without any false-positive results at any reading time.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Conclusions<\/jats:title>\n                  <jats:p>In healthcare settings with a high prevalence of OXA-48 producers, the inclusion of ertapenem and temocillin discs in the sRAST procedure may improve the detection of OXA-48-producing K. pneumoniae isolates directly from positive BCs, providing reliable results after only a 4 h incubation period.<\/jats:p>\n               <\/jats:sec>","DOI":"10.1093\/jac\/dkad406","type":"journal-article","created":{"date-parts":[[2023,12,28]],"date-time":"2023-12-28T12:43:13Z","timestamp":1703767393000},"page":"462-466","source":"Crossref","is-referenced-by-count":3,"title":["Usefulness of inclusion of ertapenem and temocillin screening breakpoints in the EUCAST rapid antimicrobial susceptibility testing (RAST) for rapid detection of OXA-48-producing <i>Klebsiella pneumoniae<\/i> directly from positive blood cultures"],"prefix":"10.1093","volume":"79","author":[{"given":"Bel\u00e9n","family":"Fern\u00e1ndez-Caso","sequence":"first","affiliation":[{"name":"Servicio de Microbiolog\u00eda, Hospital Valle del Nal\u00f3n , Langreo , Spain"},{"name":"Servicio de Microbiolog\u00eda, Hospital Universitario de Cabue\u00f1es , Gij\u00f3n , Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pilar","family":"Lumbreras-Iglesias","sequence":"additional","affiliation":[{"name":"Grupo de Neoplasias Hematol\u00f3gicas, Instituto de Investigaci\u00f3n Sanitaria del Principado de Asturias (ISPA) , Oviedo , Spain"},{"name":"Grupo de Microbiolog\u00eda Traslacional, Instituto de Investigaci\u00f3n Sanitaria del Principado de Asturias (ISPA) , Oviedo , Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M Rosario","family":"Rodicio","sequence":"additional","affiliation":[{"name":"Grupo de Microbiolog\u00eda Traslacional, Instituto de Investigaci\u00f3n Sanitaria del Principado de Asturias (ISPA) , Oviedo , Spain"},{"name":"Departamento de Biolog\u00eda Funcional, \u00c1rea de Microbiolog\u00eda, Universidad de Oviedo , Oviedo , Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Javier","family":"Fern\u00e1ndez","sequence":"additional","affiliation":[{"name":"Grupo de Microbiolog\u00eda Traslacional, Instituto de Investigaci\u00f3n Sanitaria del Principado de Asturias (ISPA) , Oviedo , Spain"},{"name":"Servicio de Microbiolog\u00eda, Hospital Universitario Central de Asturias (HUCA) , Oviedo , Spain"},{"name":"Research & Innovation, Artificial Intelligence and Statistical Department, Pragmatech , Oviedo , 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