{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,16]],"date-time":"2026-07-16T00:44:12Z","timestamp":1784162652811,"version":"3.55.0"},"reference-count":31,"publisher":"Oxford University Press (OUP)","issue":"3","license":[{"start":{"date-parts":[[2024,1,10]],"date-time":"2024-01-10T00:00:00Z","timestamp":1704844800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2024,3,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:sec>\n                  <jats:title>Objectives<\/jats:title>\n                  <jats:p>Haemophilus parainfluenzae is an opportunistic pathogen causing respiratory tract infection and sexually transmitted diseases. The emergence of multidrug resistance in this species is particularly worrisome, especially since the recent description of CTX-M-15 ESBL-producing isolates in Spain. The aim of this study was to characterize a CTX-M-15-producing H. parainfluenzae clinical isolate, HP01, obtained from a urethral swab.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Methods<\/jats:title>\n                  <jats:p>MICs were determined with gradient strips for this isolate. Hydrolysis assays were performed with the \u03b2 LACTA test. Genomic DNA from HP01 was subjected to Illumina and Oxford Nanopore sequencing to investigate the genetic environment of blaCTX-M-15. Phylogenetic analysis was performed with available H. parainfluenzae genomes from the NCBI database, including CTX-M-15 producers.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Results<\/jats:title>\n                  <jats:p>HP01, an XDR isolate, was resistant to penicillin, third-generation cephalosporins, fluoroquinolones, macrolides, cyclines and co-trimoxazole and susceptible only to carbapenems and rifampicin. HP01 carried blaTEM-1, blaCTX-M-15, tet(M), catS and mef(E)\/mel and harboured amino acid substitutions in PBP3, PBP5, GyrA, ParC and FolA implicated in resistance. Genomic analysis revealed that blaCTX-M-15 was carried by a Tn3-like transposon inserted into a novel integrative and conjugative element (ICE), ICEHpaSLS, present on the chromosome and belonging to the ICEHin1056 family described in Haemophilus influenzae. The tet(M)-MEGA element was also detected on the chromosome. No plasmid was found. The phylogenetic analysis showed that four H. parainfluenzae producing CTX-M-15 clustered in the same clade.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Conclusions<\/jats:title>\n                  <jats:p>Here we report the description of an XDR H. parainfluenzae producing blaCTX-M-15 isolated from a urethral swab. The blaCTX-M-15 gene was inserted into an ICE structure similar to those recently described in CTX-M-15 producers in Spain. The emergence of XDR H. parainfluenzae producing blaCTX-M-15 is a matter of great concern. Careful surveillance is required to prevent its spread.<\/jats:p>\n               <\/jats:sec>","DOI":"10.1093\/jac\/dkad408","type":"journal-article","created":{"date-parts":[[2024,1,10]],"date-time":"2024-01-10T12:46:35Z","timestamp":1704890795000},"page":"539-545","source":"Crossref","is-referenced-by-count":9,"title":["Detection of CTX-M-15 ESBL in XDR <i>Haemophilus parainfluenzae<\/i> from a urethral swab"],"prefix":"10.1093","volume":"79","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-0958-4802","authenticated-orcid":false,"given":"Fran\u00e7ois","family":"Cam\u00e9l\u00e9na","sequence":"first","affiliation":[{"name":"Universit\u00e9 de Paris Cit\u00e9, INSERM, IAME , Paris F-75018 , France"},{"name":"Department of Bacteriology, Saint-Louis-Lariboisi\u00e8re Hospital Group, Assistance Publique\u2014H\u00f4pitaux de Paris , Paris F-75010 , France"},{"name":"French National Reference Centre for Bacterial STIs, Associated Laboratory for Gonococci , Paris F-75010 , France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Manel","family":"Merim\u00e8che","sequence":"additional","affiliation":[{"name":"Universit\u00e9 de Paris Cit\u00e9, INSERM, IAME , Paris F-75018 , France"},{"name":"Department of Bacteriology, Saint-Louis-Lariboisi\u00e8re Hospital Group, Assistance Publique\u2014H\u00f4pitaux de Paris , Paris F-75010 , France"},{"name":"French National Reference Centre for Bacterial STIs, Associated Laboratory for Gonococci , Paris F-75010 , France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mathilde","family":"Liberge","sequence":"additional","affiliation":[{"name":"Universit\u00e9 de Paris Cit\u00e9, INSERM, IAME , Paris F-75018 , France"},{"name":"Department of Bacteriology, Saint-Louis-Lariboisi\u00e8re Hospital Group, Assistance Publique\u2014H\u00f4pitaux de Paris , Paris F-75010 , France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Clara","family":"Maubaret","sequence":"additional","affiliation":[{"name":"Universit\u00e9 de Paris Cit\u00e9, INSERM, IAME , Paris F-75018 , France"},{"name":"Department of Bacteriology, Saint-Louis-Lariboisi\u00e8re Hospital Group, Assistance Publique\u2014H\u00f4pitaux de Paris , Paris F-75010 , France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jean-Luc","family":"Donay","sequence":"additional","affiliation":[{"name":"Department of Bacteriology, Saint-Louis-Lariboisi\u00e8re Hospital Group, Assistance Publique\u2014H\u00f4pitaux de Paris , Paris F-75010 , France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Muhamed-Kheir","family":"Taha","sequence":"additional","affiliation":[{"name":"Invasive Bacterial Infections Unit and National Reference Centre for Haemophilus influenzae, Institut Pasteur , Paris F-75015 , 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