{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,11]],"date-time":"2026-10-11T03:19:46Z","timestamp":1791688786436,"version":"4.3.4"},"reference-count":34,"publisher":"Oxford University Press (OUP)","issue":"3","license":[{"start":{"date-parts":[[2024,2,2]],"date-time":"2024-02-02T00:00:00Z","timestamp":1706832000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Proyectos I + D + i Agentes del Sistema Andaluz del Conocimiento"},{"DOI":"10.13039\/501100011011","name":"Junta de Andaluc\u00eda","doi-asserted-by":"publisher","award":["P20-00985"],"award-info":[{"award-number":["P20-00985"]}],"id":[{"id":"10.13039\/501100011011","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100011011","name":"Junta de Andaluc\u00eda","doi-asserted-by":"publisher","award":["RH-0136-2020"],"award-info":[{"award-number":["RH-0136-2020"]}],"id":[{"id":"10.13039\/501100011011","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Modalidad contrato postdoctoral especialista"},{"name":"Consejer\u00eda de Salud y Familias"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2024,3,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:sec>\n                  <jats:title>Background<\/jats:title>\n                  <jats:p>BaeS\/BaeR is a two-component system of Escherichia coli that controls the expression of porins and efflux pumps. Its role in beta-lactam resistance is limited.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Objectives<\/jats:title>\n                  <jats:p>To study the role of baeS\/baeR two-component system in temocillin resistance in E. coli.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Methods<\/jats:title>\n                  <jats:p>E. coli strain BW25113 and single-gene deletion mutants related to two-component systems were collected from the KEIO collection. Double-gen deletion mutants were generated. Temocillin-resistant mutant frequencies were determined at 32\u2005mg\/L. E. coli BW25113 mutants were selected by selective pressure from serial passages. Biological costs were analysed by growth curves. Genomes of the generated mutants were sequenced. The expression level of the mdtA, mdtB, mdtC, acrD and tolC in the \u0394baeS mutant was determined by RT\u2013PCR (with\/without temocillin exposure).<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Results<\/jats:title>\n                  <jats:p>The frequency of temocillin mutants ranged from 2.12 \u00d7 10\u22128 to 4.51 \u00d7 10\u22128 in single-porin mutants. No mutants were recovered from E. coli BW25113 (&amp;gt;10\u22129). Selection of temocillin-resistant variants by serial passage yielded mutants up to 128\u2005mg\/L. Mutations were found in the baeS gene. Temocillin MICs ranged from 4 to 32\u2005mg\/L (highest MICs for \u0394baeS and \u0394ompR). The efflux pumps mdtA, mdtB, mdtC and acrD pumps were overexpressed 3\u201310-fold in the presence of temocillin in \u0394baeS compared to control.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Conclusions<\/jats:title>\n                  <jats:p>Mutations in the sensor histidine kinase, baeS, may be involved in temocillin resistance through the expression of the efflux pumps mdtABC and acrD. In addition, the low mutation rate may be a good predictor of temocillin activity.<\/jats:p>\n               <\/jats:sec>","DOI":"10.1093\/jac\/dkae021","type":"journal-article","created":{"date-parts":[[2024,2,2]],"date-time":"2024-02-02T14:41:16Z","timestamp":1706884876000},"page":"641-647","source":"Crossref","is-referenced-by-count":14,"title":["Implications of two-component systems <i>EnvZ<\/i>\/<i>OmpR<\/i> and <i>BaeS<\/i>\/<i>BaeR<\/i> in <i>in vitro<\/i> temocillin resistance in <i>Escherichia coli<\/i>"],"prefix":"10.1093","volume":"79","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3775-5330","authenticated-orcid":false,"given":"Patricia","family":"P\u00e9rez-Palacios","sequence":"first","affiliation":[{"name":"Unidad Cl\u00ednica de Enfermedades Infecciosas y Microbiolog\u00eda, Hospital Universitario Virgen Macarena , Seville , Spain"},{"name":"Instituto de Biomedicina de Sevilla IBIS, Hospital Universitario Virgen Macarena\/CSIC\/Universidad de Sevilla , Sevilla , 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