{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,25]],"date-time":"2026-08-25T00:12:54Z","timestamp":1787616774480,"version":"build-2736575974"},"reference-count":23,"publisher":"Oxford University Press (OUP)","issue":"4","license":[{"start":{"date-parts":[[2024,2,9]],"date-time":"2024-02-09T00:00:00Z","timestamp":1707436800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"funder":[{"DOI":"10.13039\/501100001411","name":"Indian Council of Medical Research","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001411","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001332","name":"CSIR","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001332","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2024,4,2]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:sec>\n                  <jats:title>Background<\/jats:title>\n                  <jats:p>The clinical relevance of Acinetobacter pittii is increasing, but reports of this organism causing neonatal sepsis are rare.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Objectives<\/jats:title>\n                  <jats:p>To understand the mechanisms of resistance and virulence of A. pittii isolated from neonatal blood belonging to a novel sequence type.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Materials and methods<\/jats:title>\n                  <jats:p>Antibiotic susceptibility, MLST, WGS, phylogenomic comparison with a global collection of carbapenemase-harbouring A. pittii were done. To study the pathogenic potential of novel A. pittii, in vitro and in vivo assays were carried out.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Results and discussion<\/jats:title>\n                  <jats:p>Two novel multidrug-resistant A. pittii from neonatal blood belonging to a novel sequence type 1451 (ST1451) were isolated. WGS revealed that the isolates were almost similar (147 SNP distant) and harbouring two carbapenem resistance genes blaNDM-1 with upstream ISAba125 and downstream bleMBL along with blaOXA-58 with upstream ISAba3. Other resistance genes included blaADC-25, blaOXA-533, aph(3\u2033)-Ib, aph(3\u2032)-VIa, aph(6)-Id, aac(3)-IId, mph(E), msr(E), sul2 and tet(39), different efflux pump genes and amino acid substitutions within GyrA (Ser81Leu) and ParC (Ser84Leu; Glu88Ala) were detected among the isolates. The study genomes were closely related to four strains belonging to ST119. The isolates showed biofilm production, serum resistance, growth under iron limiting condition, surface-associated motility and adherence to host cell. Isolates induced cytokine production in the host cell and showed mice mortality.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Discussion and conclusions<\/jats:title>\n                  <jats:p>This study is the first report of the presence of blaNDM-1 in A. pittii from India along with another carbapenemase blaOXA-58. Emergence of highly virulent, multidrug-resistant A. pittii with attributes similar to A. baumannii calls for surveillance to identify the novel strains and their pathogenic and resistance potential.<\/jats:p>\n               <\/jats:sec>","DOI":"10.1093\/jac\/dkae024","type":"journal-article","created":{"date-parts":[[2024,2,9]],"date-time":"2024-02-09T14:24:16Z","timestamp":1707488656000},"page":"779-783","source":"Crossref","is-referenced-by-count":5,"title":["Novel sequence type of carbapenem-resistant <i>Acinetobacter pittii<\/i> ST1451 with enhanced virulence isolated from septicaemic neonates in India"],"prefix":"10.1093","volume":"79","author":[{"given":"Subhasree","family":"Roy","sequence":"first","affiliation":[{"name":"Division of Bacteriology, ICMR-National Institute of Cholera and Enteric Diseases , Kolkata , India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daichi","family":"Morita","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Graduate School of Biomedical and Health Sciences, Hiroshima University , Hiroshima , Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sushmita","family":"Bhattacharya","sequence":"additional","affiliation":[{"name":"Division of Biochemistry, ICMR-National Institute of Cholera and Enteric Diseases , Kolkata , India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shanta","family":"Dutta","sequence":"additional","affiliation":[{"name":"Division of Bacteriology, ICMR-National Institute of Cholera and Enteric Diseases , Kolkata , India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7811-7140","authenticated-orcid":false,"given":"Sulagna","family":"Basu","sequence":"additional","affiliation":[{"name":"Division of Bacteriology, ICMR-National Institute of Cholera and Enteric Diseases , Kolkata , 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