{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,30]],"date-time":"2026-04-30T16:47:18Z","timestamp":1777567638490,"version":"3.51.4"},"reference-count":20,"publisher":"Oxford University Press (OUP)","issue":"8","license":[{"start":{"date-parts":[[2024,6,29]],"date-time":"2024-06-29T00:00:00Z","timestamp":1719619200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"funder":[{"DOI":"10.13039\/501100006342","name":"Vaincre la Mucoviscidose","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100006342","id-type":"DOI","asserted-by":"publisher"}]},{"name":"l\u2019Association Gr\u00e9gory Lemarchal","award":["RF20200502683"],"award-info":[{"award-number":["RF20200502683"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2024,8,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:sec>\n                  <jats:title>Objectives<\/jats:title>\n                  <jats:p>Mycobacterium abscessus has emerged as an opportunistic pathogen responsible for lung infections, especially in cystic fibrosis patients. In spite of the production of the broad-spectrum \u03b2-lactamase BlaMab, the carbapenem imipenem is recommended in the initial phase of the treatment of pulmonary infections. Here, we determine whether the addition of vaborbactam, a second-generation \u03b2-lactamase inhibitor belonging to the boronate family, improves the activity of \u03b2-lactams against M. abscessus.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Methods<\/jats:title>\n                  <jats:p>The activity of \u03b2-lactams, alone or in combination with vaborbactam, was evaluated against M. abscessus CIP104536 by determining MICs, time-killing and intramacrophage activity. Kinetic parameters for the inhibition of BlaMab by vaborbactam were determined by spectrophotometry.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Results<\/jats:title>\n                  <jats:p>The combination of vaborbactam (8 mg\/L) with \u03b2-lactams decreased more than 8 times the MIC of amoxicillin (from &amp;gt;1024 to 128 mg\/L) and 2 times the MICs of meropenem (from 16 to 8 mg\/L) and imipenem (from 4 to 2 mg\/L). The reduction of the MICs was less than that obtained with avibactam at 4 mg\/L for amoxicillin (from &amp;gt;1024 to 16 mg\/L, more than 64 times less) and for meropenem (from 16 to 4 mg\/L, 4 times less). In vitro and intracellularly, M. abscessus was not killed by the meropenem\/vaborbactam combination, in spite of significant in vitro inhibition of BlaMab by vaborbactam.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Conclusions<\/jats:title>\n                  <jats:p>Inhibition of BlaMab by vaborbactam decreases the MIC of \u03b2-lactams, including that of meropenem. As meropenem\/vaborbactam is clinically available, this combination offers an alternative therapeutic option that should be evaluated for the treatment of pulmonary infections due to M. abscessus.<\/jats:p>\n               <\/jats:sec>","DOI":"10.1093\/jac\/dkae181","type":"journal-article","created":{"date-parts":[[2024,6,29]],"date-time":"2024-06-29T11:44:46Z","timestamp":1719661486000},"page":"1914-1918","source":"Crossref","is-referenced-by-count":2,"title":["<i>In vitro<\/i> and intracellular activity of vaborbactam combined with \u03b2-lactams against <i>Mycobacterium abscessus<\/i>"],"prefix":"10.1093","volume":"79","author":[{"given":"L\u00e9a","family":"Sanchez","sequence":"first","affiliation":[{"name":"Centre de Recherche des Cordeliers, INSERM, Sorbonne Universit\u00e9, Universit\u00e9 Paris Cit\u00e9 , Paris F-75006 , France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maria","family":"Bitar","sequence":"additional","affiliation":[{"name":"Centre de Recherche des Cordeliers, INSERM, Sorbonne Universit\u00e9, Universit\u00e9 Paris Cit\u00e9 , Paris F-75006 , France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Quentin","family":"Herail","sequence":"additional","affiliation":[{"name":"Centre de Recherche des Cordeliers, INSERM, Sorbonne Universit\u00e9, Universit\u00e9 Paris Cit\u00e9 , Paris F-75006 , France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Delphine","family":"Dorch\u00eane","sequence":"additional","affiliation":[{"name":"Centre de Recherche des Cordeliers, INSERM, Sorbonne Universit\u00e9, Universit\u00e9 Paris Cit\u00e9 , Paris F-75006 , France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jean-Emmanuel","family":"Hugonnet","sequence":"additional","affiliation":[{"name":"Centre de Recherche des Cordeliers, INSERM, Sorbonne Universit\u00e9, Universit\u00e9 Paris Cit\u00e9 , Paris F-75006 , France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1007-636X","authenticated-orcid":false,"given":"Michel","family":"Arthur","sequence":"additional","affiliation":[{"name":"Centre de Recherche des Cordeliers, INSERM, Sorbonne Universit\u00e9, Universit\u00e9 Paris Cit\u00e9 , Paris F-75006 , France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jean-Luc","family":"Mainardi","sequence":"additional","affiliation":[{"name":"Centre de Recherche des Cordeliers, INSERM, Sorbonne Universit\u00e9, Universit\u00e9 Paris Cit\u00e9 , Paris F-75006 , France"},{"name":"Assistance Publique-H\u00f4pitaux de Paris, Centre-Universit\u00e9 de Paris, Universit\u00e9 Paris Cit\u00e9, Service de Microbiologie, H\u00f4pital Europ\u00e9en Georges Pompidou , Paris F-75015 , France"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[2024,6,29]]},"reference":[{"key":"2024073123513827800_dkae181-B1","doi-asserted-by":"crossref","first-page":"53","DOI":"10.1016\/j.jcf.2014.05.008","article-title":"Increasing nontuberculous mycobacteria infection in cystic fibrosis","volume":"14","author":"Bar-On","year":"2015","journal-title":"J Cyst Fibros"},{"key":"2024073123513827800_dkae181-B2","doi-asserted-by":"crossref","first-page":"691","DOI":"10.1093\/jac\/dkt410","article-title":"Characterization of broad-spectrum Mycobacterium abscessus class A \u03b2-lactamase","volume":"69","author":"Soroka","year":"2014","journal-title":"J Antimicrob Chemother"},{"key":"2024073123513827800_dkae181-B3","doi-asserted-by":"crossref","first-page":"1051","DOI":"10.1093\/jac\/dku510","article-title":"\u03b2-Lactamase inhibition by avibactam in Mycobacterium abscessus","volume":"70","author":"Dub\u00e9e","year":"2015","journal-title":"J Antimicrob Chemother"},{"key":"2024073123513827800_dkae181-B4","doi-asserted-by":"crossref","first-page":"i1","DOI":"10.1136\/thoraxjnl-2015-207360","article-title":"US Cystic Fibrosis Foundation and European Cystic Fibrosis Society consensus recommendations for the management of non-tuberculous mycobacteria in individuals with cystic fibrosis","volume":"71","author":"Floto","year":"2016","journal-title":"Thorax"},{"key":"2024073123513827800_dkae181-B5","first-page":"1081","article-title":"Inhibition of \u03b2-lactamases of mycobacteria by avibactam and clavulanate","volume":"72","author":"Soroka","year":"2017","journal-title":"J Antimicrob Chemother"},{"key":"2024073123513827800_dkae181-B6","first-page":"379","article-title":"Impact of relebactam-mediated inhibition of Mycobacterium abscessus BlaMab \u03b2-lactamase on the in vitro and intracellular efficacy of imipenem","volume":"75","author":"Le 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potencies against drug-resistant Mycobacterium abscessus","volume":"12","author":"Kaushik","year":"2017","journal-title":"Future Microbiol"},{"key":"2024073123513827800_dkae181-B10","doi-asserted-by":"crossref","first-page":"e01915-18","DOI":"10.1128\/AAC.01915-18","article-title":"In vitro and intracellular activity of imipenem combined with tedizolid, rifabutin, and avibactam against Mycobacterium abscessus","volume":"63","author":"Le Run","year":"2019","journal-title":"Antimicrob Agents Chemother"},{"key":"2024073123513827800_dkae181-B11","doi-asserted-by":"crossref","first-page":"e02623-18","DOI":"10.1128\/AAC.02623-18","article-title":"In vitro activity of the new \u03b2-lactamase inhibitors relebactam and vaborbactam in combination with \u03b2-lactams against Mycobacterium abscessus complex clinical isolates","volume":"63","author":"Kaushik","year":"2019","journal-title":"Antimicrob Agents Chemother"},{"key":"2024073123513827800_dkae181-B12","doi-asserted-by":"crossref","first-page":"e00733-19","DOI":"10.1128\/AAC.00733-19","article-title":"New beta-lactamase inhibitors nacubactam and zidebactam improve the in vitro activity of beta-lactam antibiotics against Mycobacterium abscessus complex clinical isolates","volume":"63","author":"Kaushik","year":"2019","journal-title":"Antimicrob Agents Chemother"},{"key":"2024073123513827800_dkae181-B13","doi-asserted-by":"crossref","first-page":"e0352921","DOI":"10.1128\/mbio.03529-21","article-title":"Inhibiting Mycobacterium abscessus cell wall synthesis: using a novel diazabicyclooctane \u03b2-lactamase inhibitor to augment \u03b2-lactam action","volume":"13","author":"Dousa","year":"2022","journal-title":"mBio"},{"key":"2024073123513827800_dkae181-B14","doi-asserted-by":"crossref","first-page":"e0052823","DOI":"10.1128\/aac.00528-23","article-title":"The in vitro effect of new combinations of carbapenem-\u03b2-lactamase inhibitors for Mycobacterium abscessus","volume":"67","author":"Fr\u00f6berg","year":"2023","journal-title":"Antimicrob Agents Chemother"},{"key":"2024073123513827800_dkae181-B15","doi-asserted-by":"crossref","first-page":"1556","DOI":"10.1093\/jac\/dkw022","article-title":"Bactericidal and intracellular activity of \u03b2-lactams against Mycobacterium abscessus","volume":"71","author":"Lefevbre","year":"2016","journal-title":"J Antimicrob Chemother"},{"key":"2024073123513827800_dkae181-B16","doi-asserted-by":"crossref","first-page":"e01443-17","DOI":"10.1128\/AAC.01443-17","article-title":"Vaborbactam: spectrum of beta-lactamase inhibition and impact of resistance mechanisms on activity in Enterobacteriaceae","volume":"61","author":"Lomovskaya","year":"2017","journal-title":"Antimicrob Agents Chemother"},{"key":"2024073123513827800_dkae181-B17","article-title":"Soci\u00e9t\u00e9 Fran\u00e7aise de Microbiologie Ed. p.24. 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