{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,14]],"date-time":"2026-02-14T02:09:50Z","timestamp":1771034990152,"version":"3.50.1"},"reference-count":32,"publisher":"Oxford University Press (OUP)","issue":"11","license":[{"start":{"date-parts":[[2024,9,6]],"date-time":"2024-09-06T00:00:00Z","timestamp":1725580800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"funder":[{"name":"Nyckelfonden at O\u0308rebro University Hospital","award":["OLL-502241"],"award-info":[{"award-number":["OLL-502241"]}]},{"name":"Region V\u00e4stmanland"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2024,11,4]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:sec>\n                  <jats:title>Background<\/jats:title>\n                  <jats:p>There are limited treatment options for prosthetic joint infections (PJI) due to multidrug-resistant Staphylococcus epidermidis (MDRSE). Fosfomycin (FOF) has gained attention as a potential therapy, but there is a paucity of information on the phenotypic and genotypic susceptibility amongst S. epidermidis, including MDRSE.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Objectives<\/jats:title>\n                  <jats:p>To investigate phenotypical and genotypical susceptibility to FOF in S. epidermidis isolates prospectively collected from PJIs in Sweden.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Methods<\/jats:title>\n                  <jats:p>MIC determination was performed using in-house agar dilution (AD) and a commercial AD panel. Genes and gene variants associated with FOF resistance were analysed.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Results<\/jats:title>\n                  <jats:p>Multidrug resistance was common [74\/89 (83%) isolates were MDRSE].<\/jats:p>\n                  <jats:p>FOF inhibited all isolates except one, which had an MIC &amp;gt; 256\u2005mg\/L. The commercial AD panel demonstrated good overall performance but tended to overestimate the MIC, resulting in 84% essential agreement with the gold standard. Genomic analysis with publically available tools for whole-genome sequencing (WGS) data suggested genotypic FOF resistance in all isolates, but in-depth analysis revealed that fosB, associated with FOF resistance, was only present in the phenotypically resistant isolate. No other genes or gene variants associated with FOF resistance were detected.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Conclusions<\/jats:title>\n                  <jats:p>Phenotypic resistance to FOF and presence of fosB were rare in this collection, indicating FOF\u2019s potential as a treatment option for S. epidermidis. The commercial AD panel demonstrated high reproducibility, but EA with the reference method was less than optimal. Findings of genotypic FOF resistance using common tools for WGS data should be critically evaluated and appropriately verified with relevant fosB references for S. epidermidis.<\/jats:p>\n               <\/jats:sec>","DOI":"10.1093\/jac\/dkae312","type":"journal-article","created":{"date-parts":[[2024,9,7]],"date-time":"2024-09-07T01:11:19Z","timestamp":1725671479000},"page":"2948-2953","source":"Crossref","is-referenced-by-count":4,"title":["Probing fosfomycin\u2019s potential: a study on susceptibility testing and resistance in <i>Staphylococcus epidermidis<\/i> from prosthetic joint infections"],"prefix":"10.1093","volume":"79","author":[{"given":"Rebecka","family":"Widerstr\u00f6m","sequence":"first","affiliation":[{"name":"Department of Laboratory Medicine, Region V\u00e4stmanland , V\u00e4ster\u00e5s ,","place":["Sweden"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0001-7074-3219","authenticated-orcid":false,"given":"Mia","family":"Aarris","sequence":"additional","affiliation":[{"name":"Department of Bioinformatics, Statens Serum Institut , Copenhagen ,","place":["Denmark"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Susanne","family":"Jacobsson","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine, Faculty of Medicine and Health, \u00d6rebro University , \u00d6rebro ,","place":["Sweden"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marc","family":"Stegger","sequence":"additional","affiliation":[{"name":"Department of Bioinformatics, Statens Serum Institut , Copenhagen ,","place":["Denmark"]},{"name":"School of Medical Sciences, Faculty of Medicine and Health, \u00d6rebro University , \u00d6rebro ,","place":["Sweden"]},{"name":"Antimicrobial Resistance and Infectious Diseases Laboratory, Harry Butler Institute, Murdoch University , Perth ,","place":["Australia"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5939-2932","authenticated-orcid":false,"given":"Bo","family":"S\u00f6derquist","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine, Faculty of Medicine and Health, \u00d6rebro University , \u00d6rebro ,","place":["Sweden"]},{"name":"School of Medical Sciences, Faculty of Medicine and Health, \u00d6rebro University , \u00d6rebro ,","place":["Sweden"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Emeli","family":"M\u00e5nsson","sequence":"additional","affiliation":[{"name":"Region V\u00e4stmanland \u2013 Uppsala University, Centre for Clinical Research, V\u00e4stmanland Hospital V\u00e4steras , V\u00e4ster\u00e5s ,","place":["Sweden"]}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[2024,9,6]]},"reference":[{"key":"2024110323523434400_dkae312-B1","doi-asserted-by":"publisher","first-page":"321","DOI":"10.1128\/CMR.00068-15","article-title":"Fosfomycin","volume":"29","author":"Falagas","year":"2016","journal-title":"Clin Microbiol Rev"},{"key":"2024110323523434400_dkae312-B2","doi-asserted-by":"publisher","first-page":"e104","DOI":"10.1016\/S2666-5247(23)00339-7","article-title":"Global trends in the increasing prevalence of the fosfomycin resistance gene in Staphylococcus aureus","volume":"5","author":"Monte","year":"2024","journal-title":"Lancet Microbe"},{"key":"2024110323523434400_dkae312-B3","doi-asserted-by":"publisher","first-page":"66","DOI":"10.1186\/1471-2334-14-66","article-title":"In vitro susceptibility of Escherichia coli strains isolated from urine samples obtained in mainland China to fosfomycin trometamol and other antibiotics: a 9-year surveillance study (2004-2012)","volume":"14","author":"Lai","year":"2014","journal-title":"BMC Infect Dis"},{"key":"2024110323523434400_dkae312-B4","doi-asserted-by":"publisher","first-page":"1893","DOI":"10.1128\/JCM.03484-13","article-title":"Fosfomycin susceptibility in carbapenem-resistant Enterobacteriaceae from Germany","volume":"52","author":"Kaase","year":"2014","journal-title":"J Clin Microbiol"},{"key":"2024110323523434400_dkae312-B5","doi-asserted-by":"publisher","first-page":"505","DOI":"10.1038\/ja.2012.62","article-title":"In vitro susceptibility of Staphylococcus aureus and Staphylococcus epidermidis isolated from prosthetic joint infections","volume":"65","author":"Molina-Manso","year":"2012","journal-title":"J Antibiot (Tokyo)"},{"key":"2024110323523434400_dkae312-B6","doi-asserted-by":"publisher","first-page":"269","DOI":"10.1016\/j.ijantimicag.2016.02.001","article-title":"Susceptibility of contemporary isolates to fosfomycin: a systematic review of the literature","volume":"47","author":"Vardakas","year":"2016","journal-title":"Int J Antimicrob Agents"},{"key":"2024110323523434400_dkae312-B7","doi-asserted-by":"publisher","first-page":"521","DOI":"10.1016\/j.ijantimicag.2013.02.018","article-title":"In vitro susceptibility to antibiotics of staphylococci in biofilms isolated from orthopaedic infections","volume":"41","author":"Molina-Manso","year":"2013","journal-title":"Int J Antimicrob Agents"},{"key":"2024110323523434400_dkae312-B8","doi-asserted-by":"publisher","first-page":"6413982","DOI":"10.1155\/2016\/6413982","article-title":"Efficacy of linezolid and fosfomycin in catheter-related biofilm infection caused by methicillin-resistant Staphylococcus aureus","volume":"2016","author":"Chai","year":"2016","journal-title":"Biomed Res Int"},{"issue":"Suppl 1","key":"2024110323523434400_dkae312-B9","first-page":"30","article-title":"The role of fosfomycin in osteoarticular infection","volume":"32","author":"Morata","year":"2019","journal-title":"Rev Esp Quimioter"},{"key":"2024110323523434400_dkae312-B10","doi-asserted-by":"publisher","first-page":"207","DOI":"10.7150\/jbji.27811","article-title":"Daptomycin plus fosfomycin as salvage therapy in a difficult-to-treat total femoral replacement infection","volume":"3","author":"Luengo","year":"2018","journal-title":"J Bone Jt Infect"},{"key":"2024110323523434400_dkae312-B11","doi-asserted-by":"publisher","first-page":"182","DOI":"10.3109\/00365548.2011.616221","article-title":"Fosfomycin: efficacy against infections caused by multidrug-resistant bacteria","volume":"44","author":"Dinh","year":"2012","journal-title":"Scand J Infect Dis"},{"key":"2024110323523434400_dkae312-B12","doi-asserted-by":"publisher","first-page":"22","DOI":"10.1016\/j.ijantimicag.2018.09.013","article-title":"Resistance to fosfomycin: mechanisms, frequency and clinical consequences","volume":"53","author":"Falagas","year":"2019","journal-title":"Int J Antimicrob Agents"},{"key":"2024110323523434400_dkae312-B13","doi-asserted-by":"publisher","first-page":"217","DOI":"10.3390\/antibiotics2020217","article-title":"Molecular mechanisms and clinical impact of acquired and intrinsic fosfomycin resistance","volume":"2","author":"Castaneda-Garcia","year":"2013","journal-title":"Antibiotics (Basel)"},{"key":"2024110323523434400_dkae312-B14","doi-asserted-by":"publisher","first-page":"135","DOI":"10.1186\/s13756-020-00790-x","article-title":"Prevalence of fosfomycin resistance and gene mutations in clinical isolates of methicillin-resistant Staphylococcus aureus","volume":"9","author":"Lee","year":"2020","journal-title":"Antimicrob Resist Infect Control"},{"key":"2024110323523434400_dkae312-B15","doi-asserted-by":"publisher","first-page":"000504","DOI":"10.1099\/mgen.0.000504","article-title":"Comparative genomics of Staphylococcus epidermidis from prosthetic-joint infections and nares highlights genetic traits associated with antimicrobial resistance, not virulence","volume":"7","author":"Mansson","year":"2021","journal-title":"Microb Genom"},{"key":"2024110323523434400_dkae312-B16","doi-asserted-by":"publisher","first-page":"000748","DOI":"10.1099\/mgen.0.000748","article-title":"ResFinder\u2014an open online resource for identification of antimicrobial resistance genes in next-generation sequencing data and prediction of phenotypes from genotypes","volume":"8","author":"Florensa","year":"2022","journal-title":"Microb Genom"},{"key":"2024110323523434400_dkae312-B17","doi-asserted-by":"publisher","first-page":"391","DOI":"10.1016\/j.jgar.2020.01.003","article-title":"Performance of disk diffusion and broth microdilution for fosfomycin susceptibility testing of multidrug-resistant clinical isolates of Enterobacterales and Pseudomonas aeruginosa","volume":"21","author":"Mojica","year":"2020","journal-title":"J Glob Antimicrob Resist"},{"key":"2024110323523434400_dkae312-B18","doi-asserted-by":"publisher","first-page":"2380","DOI":"10.1093\/jac\/dky214","article-title":"Susceptibility of ESBL Escherichia coli and Klebsiella pneumoniae to fosfomycin in The Netherlands and comparison of several testing methods including etest, MIC test strip, Vitek2, Phoenix and disc diffusion","volume":"73","author":"van den Bijllaardt","year":"2018","journal-title":"J Antimicrob Chemother"},{"key":"2024110323523434400_dkae312-B19","doi-asserted-by":"publisher","first-page":"700","DOI":"10.3390\/pathogens13010012","article-title":"Reliability of E-tests and the phoenix automated method in assessing susceptibility to IV fosfomycin-comparative studies relative to the reference method","volume":"12","author":"Kowalska-Krochmal","year":"2023","journal-title":"Pathogens"},{"key":"2024110323523434400_dkae312-B20","doi-asserted-by":"publisher","first-page":"334","DOI":"10.1016\/j.jgar.2020.08.025","article-title":"Gold standard susceptibility testing of fosfomycin in Staphylococcus aureus and Enterobacterales using a new agar dilution panel(R)","volume":"23","author":"Campanile","year":"2020","journal-title":"J Glob Antimicrob Resist"},{"key":"2024110323523434400_dkae312-B21","doi-asserted-by":"publisher","first-page":"e0250421","DOI":"10.1128\/spectrum.02504-21","article-title":"Fosfomycin susceptibility testing using commercial agar dilution test","volume":"10","author":"Croughs","year":"2022","journal-title":"Microbiol Spectr"},{"key":"2024110323523434400_dkae312-B22","doi-asserted-by":"publisher","first-page":"788.e5","DOI":"10.1016\/j.cmi.2020.11.029","article-title":"Evaluation of the commercial AD fosfomycin test for susceptibility testing of multidrug-resistant Enterobacterales and Pseudomonas aeruginosa","volume":"27","author":"Parisio","year":"2020","journal-title":"Clin Microbiol Infect"},{"key":"2024110323523434400_dkae312-B23","doi-asserted-by":"publisher","first-page":"143","DOI":"10.1016\/j.diagmicrobio.2018.08.010","article-title":"Activity of fosfomycin when tested against US contemporary bacterial isolates","volume":"93","author":"Flamm","year":"2019","journal-title":"Diagn Microbiol Infect Dis"},{"key":"2024110323523434400_dkae312-B24","doi-asserted-by":"publisher","first-page":"e0002122","DOI":"10.1128\/jcm.00021-22","article-title":"Multicenter evaluation of the novel ETEST fosfomycin for antimicrobial susceptibility testing of Enterobacterales, Enterococcus faecalis, and Staphylococcus species","volume":"60","author":"Goer","year":"2022","journal-title":"J Clin Microbiol"},{"key":"2024110323523434400_dkae312-B25","volume-title":"Breakpoint Tables for Interpretation of MICs and Zone Diameters, Version 14.0","author":"EUCAST","year":"2024"},{"key":"2024110323523434400_dkae312-B26","author":"International Organisation for Standardisation"},{"key":"2024110323523434400_dkae312-B27","doi-asserted-by":"publisher","first-page":"e102","DOI":"10.1002\/cpbi.102","article-title":"Using SPAdes de novo assembler","volume":"70","author":"Prjibelski","year":"2020","journal-title":"Curr Protoc Bioinformatics"},{"key":"2024110323523434400_dkae312-B28","doi-asserted-by":"publisher","first-page":"267","DOI":"10.1016\/s0378-1097(05)80052-7","article-title":"Nucleotide sequence of the fosB gene conferring fosfomycin resistance in Staphylococcus epidermidis","volume":"56","author":"Zilhao","year":"1990","journal-title":"FEMS Microbiol Lett"},{"key":"2024110323523434400_dkae312-B29","doi-asserted-by":"publisher","first-page":"60","DOI":"10.1038\/s41467-022-35713-4","article-title":"An ISO-certified genomics workflow for identification and surveillance of antimicrobial resistance","volume":"14","author":"Sherry","year":"2023","journal-title":"Nat Commun"},{"key":"2024110323523434400_dkae312-B30","volume-title":"Antimicrobial Wild Type Distributions of Microorganisms","author":"EUCAST"},{"key":"2024110323523434400_dkae312-B31","article-title":"EUCAST general consultation on iv fosfomycin breakpoints: 12 July to 12 September","author":"EUCAST","year":"2023"},{"key":"2024110323523434400_dkae312-B32","doi-asserted-by":"publisher","first-page":"595","DOI":"10.1186\/1471-2105-11-595","article-title":"BIGSdb: Scalable analysis of bacterial genome variation at the population level","volume-title":"BMC Bioinformatics","author":"Jolly","year":"2010"}],"container-title":["Journal of Antimicrobial Chemotherapy"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/academic.oup.com\/jac\/article-pdf\/79\/11\/2948\/60272000\/dkae312.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/academic.oup.com\/jac\/article-pdf\/79\/11\/2948\/60272000\/dkae312.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,11,3]],"date-time":"2024-11-03T23:52:42Z","timestamp":1730677962000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/jac\/article\/79\/11\/2948\/7750374"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,9,6]]},"references-count":32,"journal-issue":{"issue":"11","published-online":{"date-parts":[[2024,9,6]]},"published-print":{"date-parts":[[2024,11,4]]}},"URL":"https:\/\/doi.org\/10.1093\/jac\/dkae312","relation":{},"ISSN":["0305-7453","1460-2091"],"issn-type":[{"value":"0305-7453","type":"print"},{"value":"1460-2091","type":"electronic"}],"subject":[],"published-other":{"date-parts":[[2024,11]]},"published":{"date-parts":[[2024,9,6]]}}}