{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T04:10:43Z","timestamp":1790741443977,"version":"4.1.0"},"reference-count":44,"publisher":"Oxford University Press (OUP)","issue":"11","license":[{"start":{"date-parts":[[2024,9,17]],"date-time":"2024-09-17T00:00:00Z","timestamp":1726531200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"funder":[{"name":"Key Laboratory of Clinical Laboratory Diagnosis","award":["2022E10022"],"award-info":[{"award-number":["2022E10022"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2024,11,4]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:sec>\n                  <jats:title>Objectives<\/jats:title>\n                  <jats:p>To investigate the characteristics and clonal dynamics of tigecycline-resistant Acinetobacter baumannii (TRAB) isolates from a Chinese hospital from 2016 to 2021.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Methods<\/jats:title>\n                  <jats:p>A total of 64 TRAB isolates were screened and WGS was performed. Phylogenetic analysis and non-polymorphic mutation analysis were used to analyse their clonal dynamics and tigecycline resistance-related mutations. RT-PCR was used to analyse the expression of the resistance-nodulation cell-division (RND) efflux pump genes adeB and adeJ. Gene cloning was used to explore the effect of tet(39) variants on tigecycline resistance.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Results<\/jats:title>\n                  <jats:p>Most TRAB isolates were found to be MDR, with 95% (61\/64) of the isolates showing resistance to carbapenems. These TRAB isolates were classified into three primary genetic clusters based on core-genome SNPs. The KL2 cluster persisted throughout the study period, whereas the KL7 cluster emerged in 2019 and became the dominant clone. The KL7 cluster carried more antimicrobial resistance genes than the other two clusters. The predominant tigecycline resistance mechanism of the KL2 cluster and KL7 cluster was IS insertion in adeN (82.1%, 23\/28) and genetic alterations in adeS (76.2%, 16\/21), respectively. Eleven novel AdeS mutations were identified associated with elevated AdeB expression and tigecycline resistance. Moreover, we characterized a plasmid-borne tet(39) variant with an Ala-36-Thr substitution that synergizes with the RND efflux pump to confer high-level tigecycline resistance.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Conclusions<\/jats:title>\n                  <jats:p>This work provides important insights into the diverse mechanisms associated with tigecycline resistance in A. baumannii, highlighting a pressing need for further monitoring of ST2-KL7 A. baumannii in clinical settings.<\/jats:p>\n               <\/jats:sec>","DOI":"10.1093\/jac\/dkae314","type":"journal-article","created":{"date-parts":[[2024,9,17]],"date-time":"2024-09-17T22:00:51Z","timestamp":1726610451000},"page":"2965-2974","source":"Crossref","is-referenced-by-count":7,"title":["Genome analysis of tigecycline-resistant <i>Acinetobacter baumannii<\/i> reveals nosocomial lineage shifts and novel resistance mechanisms"],"prefix":"10.1093","volume":"79","author":[{"given":"Changrui","family":"Qian","sequence":"first","affiliation":[{"name":"Department of Clinical Laboratory, The First Affiliated Hospital of Wenzhou Medical University, Key Laboratory of Clinical Laboratory Diagnosis and Translational Research of Zhejiang Province , Wenzhou, Zhejiang Province ,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Panjie","family":"Hu","sequence":"additional","affiliation":[{"name":"School of Laboratory Medicine and Life Science, Wenzhou Medical University , Wenzhou, Zhejiang Province ,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wenhui","family":"Guo","sequence":"additional","affiliation":[{"name":"Department of Clinical Laboratory, The First Affiliated Hospital of Wenzhou Medical University, Key Laboratory of Clinical Laboratory Diagnosis and Translational Research of Zhejiang Province , Wenzhou, Zhejiang Province ,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yijia","family":"Han","sequence":"additional","affiliation":[{"name":"School of Laboratory Medicine and Life Science, Wenzhou Medical University , Wenzhou, Zhejiang Province ,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pingting","family":"Yu","sequence":"additional","affiliation":[{"name":"Department of Clinical Laboratory, The First Affiliated Hospital of Wenzhou Medical University, Key Laboratory of Clinical Laboratory Diagnosis and Translational Research of Zhejiang Province , Wenzhou, Zhejiang Province ,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yi","family":"Zhang","sequence":"additional","affiliation":[{"name":"School of Laboratory Medicine and Life Science, Wenzhou Medical University , Wenzhou, Zhejiang Province ,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhexiao","family":"Ma","sequence":"additional","affiliation":[{"name":"School of Laboratory Medicine and Life Science, Wenzhou Medical University , Wenzhou, Zhejiang Province ,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lijiang","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Clinical Laboratory, The First Affiliated Hospital of Wenzhou Medical University, Key Laboratory of Clinical Laboratory Diagnosis and Translational Research of Zhejiang Province , Wenzhou, Zhejiang Province ,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2171-4710","authenticated-orcid":false,"given":"Tieli","family":"Zhou","sequence":"additional","affiliation":[{"name":"Department of Clinical Laboratory, The First Affiliated Hospital of Wenzhou Medical University, Key Laboratory of Clinical 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